Colistin Methanesulfonate (sodium salt)
(Synonyms: 粘杆菌素甲基磺酸钠,Colimycin M,Polymyxin E sodium methanesulfonate) 目录号 : GC12613
Colistin Methanesulfonate (sodium salt)是一种多肽类抗生素，对铜绿假单胞菌敏感菌株具有抑制作用，MIC范围为4-16mg/l。
Cas No.:8068-28-8
Sample solution is provided at 25 µL, 10mM.
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Colistin Methanesulfonate (sodium salt) is a polypeptide antibiotic that inhibits susceptible strains of Pseudomonas aeruginosa, with MICs of 4-16mg/l [1]. In aqueous solution, Colistin Methanesulfonate undergoes hydrolysis, forming a complex mixture of partially sulfomethylated derivatives as well as colistin, which interferes with the structure and function of the bacterial outer membrane and cytoplasmic membrane[2]. Colistin Methanesulfonate has been widely used to inhibit the growth of various multi-drug resistant bacteria both in vitro and in vivo[3].
In vitro, Colistin Methanesulfonate treatment at 0.3mM for 48 hours resulted in a significant decrease in the viability of SH-SY5Y cells, along with changes in cell morphology and the aggregation of cell debris[4]. Treatment with 256mg/l Colistin Methanesulfonate for 24 hours led to an increase in the number of mesosomes in M. tuberculosis H37Ra, accompanied by a large number of cell lysis and cell debris[5].
References:
[1] Li J, Turnidge J, Milne R, et al. In vitro pharmacodynamic properties of colistin and colistin methanesulfonate against Pseudomonas aeruginosa isolates from patients with cystic fibrosis[J]. Antimicrobial agents and chemotherapy, 2001, 45(3): 781-785.
[2] Bergen P J, Li J, Rayner C R, et al. Colistin methanesulfonate is an inactive prodrug of colistin against Pseudomonas aeruginosa[J]. Antimicrobial agents and chemotherapy, 2006, 50(6): 1953-1958.
[3] Karaiskos I, Gkoufa A, Polyzou E, et al. High-dose nebulized colistin methanesulfonate and the role in hospital-acquired pneumonia caused by gram-negative bacteria with difficult-to-treat resistance: a review[J]. Microorganisms, 2023, 11(6): 1459.
[4] Lu J, Zhu Y, Li M, et al. In Vitro Neurotoxicity Comparison: Colistimethate Induces Higher Toxicity Than Colistin via Formaldehyde Accumulation[J]. International Journal of Antimicrobial Agents, 2025: 107626.
[5] Van Breda S V, Buys A, Apostolides Z, et al. The antimicrobial effect of colistin methanesulfonate on Mycobacterium tuberculosis in vitro[J]. Tuberculosis, 2015, 95(4): 440-446.
Colistin Methanesulfonate (sodium salt)是一种多肽类抗生素，对铜绿假单胞菌敏感菌株具有抑制作用，MIC范围为4-16mg/l[1]。Colistin Methanesulfonate在水溶液中会发生水解，形成包括多粘菌素E在内的多种部分磺甲基化衍生物的复杂混合物，从而干扰细菌外膜和细胞质膜的结构与功能[2]。Colistin Methanesulfonate已广泛应用于体外和体内实验中，用于抑制多种多重耐药菌的生长[3]。
在体外，使用0.3mM的Colistin Methanesulfonate处理SH-SY5Y细胞48小时，可导致细胞活力显著下降，并伴随细胞形态改变和细胞碎片聚集[4]。用256mg/l的Colistin Methanesulfonate处理结核分枝杆菌H37Ra菌株24小时后，细菌中膜样结构数量增加，同时出现大量细胞裂解和细胞碎片[5]。
| Cell experiment [1]: | |
Cell lines | SH-SY5Y cells |
Preparation Method | SH-SY5Y cells were cultured in a humidified environment at 37°C with 5% CO2, using DMEM medium supplemented with 10% fetal bovine serum (FBS). SH-SY5Y cells were seeded in 96-well tissue culture plates (1.0×104 cells per well) and allowed to adhere for 24 hours. Then, the cells were placed in untreated conditions (control group) or treated individually with colchicine (0.3mM), Colistin Methanesulfonate (0.3mM), methanesulfonate (1.5mM), or formaldehyde (initial concentration of 1.5mM, then concentration ranging from 0.05 to 1.5mM) for 6, 24, and 48 hours, respectively, and cell viability was analyzed. |
Reaction Conditions | 0.3mM; 6, 24, and 48h |
Applications | Colistin Methanesulfonate treatment inhibited the cell viability of SH-SY5Y cells in a time-dependent manner. |
References: | |
| Cas No. | 8068-28-8 | SDF | |
| 别名 | 粘杆菌素甲基磺酸钠,Colimycin M,Polymyxin E sodium methanesulfonate | ||
| 化学名 | sodium (Z)-N-((6Z,9Z)-7,10-dihydroxy-8-(1-hydroxyethyl)-5-((Z)-oxido(((1Z,4E,7Z,10Z,13Z,16Z,19Z)-2,5,11,14-tetrahydroxy-3-(1-hydroxyethyl)-12,15-diisobutyl-8,17,20-trioxido-6,9,18-tris(2-((sulfomethyl)amino)ethyl)-1,4,7,10,13,16,19-heptaazacyclotricosa-1, | ||
| Canonical SMILES | CCC(CCCC/C([O-])=N/C(/C(O)=N/C(/C(O)=N/C(/C([O-])=N/C(/C([O-])=N/C(/C([O-])=N/C(/C(O)=N/C(/C(O)=N/C(/C([O-])=N/C(/C(O)=N\1)CCNCS(O)(=O)=O)CCNCS(O)(=O)=O)CC(C)C)CC(C)C)CCNCS(O)(=O)=O)CC/N=C(O)/C1C(O)C)CCNCS(O)(=O)=O)C(O)C)CCNCS(O)(=O)=O)C.[Na+].[Na+].[Na+] | ||
| 分子式 | C58H105N16O28S5 • 5Na | 分子量 | 1749.8 |
| 溶解度 | ≥ 53.9mg/mL in Water | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 571.5 μL | 2.8575 mL | 5.7149 mL |
| 5 mM | 114.3 μL | 571.5 μL | 1.143 mL |
| 10 mM | 57.1 μL | 285.7 μL | 571.5 μL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Potency: 451ug/mg
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet