β-CGRP, human
(Synonyms: Β-降钙素基因相关肽,人,Human β-CGRP; CGRP-II (Human)) 目录号 : GC33595
β-CGRP, human是一种神经肽激素，由降钙素基因相关肽家族产生，可与降钙素受体样受体CRLR和RAMP形成复合物（CRLR/RAMP1：IC50 = 1nM；CRLR/RAMP2：IC50 = 300nM）。
Cas No.:101462-82-2
Sample solution is provided at 25 µL, 10mM.
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β-CGRP, human is a neuropeptide hormone produced by the calcitonin gene-related peptide family, which forms a complex with calcitonin receptor-like receptors CRLR and RAMP (CRLR/RAMP1: IC50 =1nM; CRLR/RAMP2: IC50 = 300nM) [1]. β-CGRP, human activates the adenylate cyclase-cAMP-PKA signaling pathway by binding to the CGRP receptor complex (composed of CALCRL and RAMP1), inducing physiological effects such as vascular smooth muscle relaxation, neuronal excitation regulation, and inflammation mediation [2-3]. β-CGRP, human is mainly used in research on migraine, cardiovascular regulation, neuroinflammation, and pain [4].
In A549 cells, β-CGRP, human (0.1-10nM; 24h) markedly increases heart rate in rats, and reduces IL-1β-induced production of IL-8 and MCP-1 [5]. In endothelial cells, β-CGRP, human (1-30μM; 24h) induces concentration-dependent relaxation of pre-contracted human intracranial arterial ring segments [6].
References:
[1]. McLatchie L M, Fraser N J, Main M J, et al. RAMPs regulate the transport and ligand specificity of the calcitonin-receptor-like receptor[J]. Nature, 1998, 393(6683): 333-339.
[2]. Bonura A, Brunelli N, Marcosano M, et al. Calcitonin gene-related peptide systemic effects: embracing the complexity of its biological roles—a narrative review[J]. International Journal of Molecular Sciences, 2023, 24(18): 13979.
[3]. Tsukiji J, Sango K, Udaka N, et al. Long-term induction of β-CGRP mRNA in rat lungs by allergic inflammation[J]. Life sciences, 2004, 76(2): 163-177.
[4]. Shen Y T, Pittman T J, Buie P S, et al. Functional role of α-calcitonin gene-related peptide in the regulation of the cardiovascular system[J]. The Journal of Pharmacology and Experimental Therapeutics, 2001, 298(2): 551-558. [1].
[5]. Li W, Hou L, Hua Z, et al. Interleukin‐1β induces β‐calcitonin gene‐related peptide secretion in human type II alveolar epithelial cells[J]. The FASEB journal, 2004, 18(13): 1603-1605.
[6]. Jansen-Olesen I, Jørgensen L, Engel U, et al. In-depth characterization of CGRP receptors in human intracranial arteries[J]. European journal of pharmacology, 2003, 481(2-3): 207-216.
β-CGRP, human是一种神经肽激素，由降钙素基因相关肽家族产生，可与降钙素受体样受体CRLR和RAMP形成复合物（CRLR/RAMP1：IC50 = 1nM；CRLR/RAMP2：IC50 = 300nM） [1]。β-CGRP, human通过与CGRP受体复合物（由CALCRL和RAMP1组成）结合，激活腺苷酸环化酶-cAMP-PKA信号通路，从而诱导血管平滑肌舒张、神经元兴奋调节和炎症介导等生理效应 [2-3]。β-CGRP, human主要用于偏头痛、心血管调节、神经炎症和疼痛的研究 [4]。
在A549细胞中，β-CGRP, human（0.1-10nM；24h）可显著增加大鼠的心率，并降低IL-1β诱导的IL-8和MCP-1的产生 [5]。在内皮细胞中，β-CGRP, human（1-30μM；24h）可诱导预收缩的人颅内动脉环段浓度依赖性舒张 [6]。
| Cell experiment [1]: | |
Cell lines | A549 cells |
Preparation Method | A549 cells were stimulated with either fresh medium, β-CGRP, human of indicated concentrations or β-CGRP, human plus IL-1ß for 24h; supernatants were collected and assayed for IL-8 or MCP-1 by ELISA. |
Reaction Conditions | 0.1-10nM; 24h |
Applications | In A549 cells, β-CGRP, human markedly increases heart rate in rats, and reduces IL-1β-induced production of IL-8 and MCP-1. |
References: | |
| Cas No. | 101462-82-2 | SDF | |
| 别名 | Β-降钙素基因相关肽,人,Human β-CGRP; CGRP-II (Human) | ||
| Canonical SMILES | Ala-Cys-Asn-Thr-Ala-Thr-Cys-Val-Thr-His-Arg-Leu-Ala-Gly-Leu-Leu-Ser-Arg-Ser-Gly-Gly-Met-Val-Lys-Ser-Asn-Phe-Val-Pro-Thr-Asn-Val-Gly-Ser-Lys-Ala-Phe-NH2(Disulfide bridge: Cys2-Cys7) | ||
| 分子式 | C162H267N51O48S3 | 分子量 | 3793.41 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 263.6 μL | 1.3181 mL | 2.6362 mL |
| 5 mM | 52.7 μL | 263.6 μL | 527.2 μL |
| 10 mM | 26.4 μL | 131.8 μL | 263.6 μL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet