Rankinidine
(Synonyms: 兰金断肠草碱) 目录号 : GC61232
Rankinidine是一种从Gelsemiumrankinii的MeOH提取物中分离得到的羟吲哚生物碱。
Cas No.:106466-66-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Rankinidine is an oxindole alkaloid that is isolated from the MeOH extract of the stem of Gelsemium rankinii[1].
[1]. Schun Y, et, al. Rankinidine, a new indole alkaloid from Gelsemium rankinii. J Nat Prod. Sep-Oct 1986; 49(5): 806-8.
| Cas No. | 106466-66-4 | SDF | |
| 别名 | 兰金断肠草碱 | ||
| Canonical SMILES | O=C1[C@]2([C@H]3C[C@]4([H])[C@@](CO3)([H])[C@@H](NC/C4=C\C)C2)C5=CC=CC=C5N1OC | ||
| 分子式 | C20H24N2O3 | 分子量 | 340.42 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9375 mL | 14.6877 mL | 29.3755 mL |
| 5 mM | 0.5875 mL | 2.9375 mL | 5.8751 mL |
| 10 mM | 0.2938 mL | 1.4688 mL | 2.9375 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A high-resolution mass spectrometric approach to a qualitative and quantitative comparative metabolism of the humantenine-type alkaloid Rankinidine
Rapid Commun Mass Spectrom 2022 Jun 30;36(12):e9302.PMID:35344234DOI:10.1002/rcm.9302.
Rationale: Rankinidine belongs to the humantenine-type alkaloids isolated from Gelsemium. Currently, the mechanism behind the toxicity differences of Rankinidine has not been explained. In this study, our purpose was to elucidate the major in vitro metabolic pathways of Rankinidine and to compare the formation of metabolites of Rankinidine in human (HLMs), rat (RLMs), goat (GLMs) and pig (PLMs) liver microsomes. Methods: This is the first study to compare the in vitro metabolism of Rankinidine with high-performance liquid chromatography/quadrupole time-of-flight mass spectrometry (LC/QTOF). The MS/MS data and LC/MS peak area acquired in positive ion mode were used to analyze metabolite structures and compare metabolism. Results: We identified 11 metabolites (M1-M11) in total and found five main metabolic pathways, consisting of demethylation (M1), reduction (M2), oxidation at different positions (M3-M5), oxidation and reduction (M6-M10) and demethylation and oxidation (M11). The metabolism of Rankinidine has qualitative and quantitative species-specific differences in vitro. In PLMs and GLMs, the main metabolic pathway of Rankinidine was oxidation. Notably, among the four species, the oxidation ability of Rankinidine was highest in pigs and goats, and the demethylation and reduction abilities of Rankinidine were highest in humans and rats. Conclusions: The interspecific metabolic differences of Rankinidine in HLMs, PLMs, GLMs and RLMs were compared and studied for the first time using LC/QTOF. These findings will certainly support future studies of Rankinidine metabolism in vivo and will contribute to elucidating the cause of species-specific differences behind Gelsemium toxicity.
Rankinidine, a new indole alkaloid from Gelsemium rankinii
J Nat Prod 1986 Sep-Oct;49(5):806-8.PMID:3819733DOI:10.1021/np50047a007.
A new oxindole alkaloid, Rankinidine (1), has been isolated from the MeOH extract of the stem of Gelsemium rankinii. Its structure was elucidated by comparison with an analog, humantenirine (3), which also occurred in this plant.