Protease Inhibitor Cocktail V (EDTA-Free, 100X in DMSO)
目录号 : GK10019蛋白酶抑制剂 V（Protease Inhibitor Cocktail V (EDTA-Free, 100X in DMSO)）相当于Sigma P9599，用于植物细胞和组织提取物以提高蛋白质稳定性。
Sample solution is provided at 25 µL, 10mM.
- View current batch:
Thaw at room temperature, add at 1:100 (v/v) dilution to solution samples (such as plant cell lysates or tissue extracts) before assaying.
Applications: WB, Co-IP, pull-down, IF, IHC, kinase assay and etc.
Endogenous proteins are produced and degraded in a balanced state, so their cellular levels are stable under stable environmental conditions. Crude cell extracts contain a number of endogenous enzymes, such as phosphatases and proteases, which are capable of degrading proteins in the extracts. Protein production is greatly halted and degradation is increased when proteins are extracted from cells and tissues in vitro. The best way to increase the yield of intact proteins is to add inhibitors of those enzymes known to be present.
Protease inhibitor cocktail is used in plant cell lysates or tissue extracts to increase protein stability. The cocktail functions to inhibit proteases that would degrade either non-phosphorylated or phosphorylated protein substrates.
This protease inhibitor cocktail contains individual components, including AEBSF, 1,10-Phenanthroline, Bestatin, E-64, Leupeptin and Pepstatin A with a broad specificity for cysteine, serine, aspartic, metalloproteases, and aminopeptidases. This protease inhibitor cocktail has been optimized and tested for plant cells and tissue extracts. This protease inhibitor cocktail is supplied as a ready-to-use solution in DMSO.
|
Ingredient |
Summary | CAS Number | Targets |
Smiles |
| AEBSF.HCl | Serine protease inhibitor | 30827-99-7 | Proteases|Serine Protease | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| Bestatin | Aminopeptidase inhibitor | 58970-76-6 | Proteases|Aminopeptidase | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| E-64 | Cysteine protease inhibitor,irriversible | 66701-25-5 | Proteases|Cathepsin | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| Leupeptin, Microbial | Inhibitor of serine and cysteine proteases | 103476-89-7 | Proteases|Serine Protease | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N([H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C(C([H])([H])[H])([H])C([H])([H])[H] |
| Pepstatin A | Aspartic proteinases inhibitor | 26305-03-3 | Proteases|Other Proteases | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C(C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| o-Phenanthroline | metalloprotease inhibitor | 66-71-7 | Proteases|Other Proteases | C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3 |
蛋白酶抑制剂混合物用于植物细胞裂解物或组织提取物以增加蛋白质稳定性。该混合物的作用是抑制会降解非磷酸化或磷酸化蛋白质底物的蛋白酶。
这种蛋白酶抑制剂混合物包含单独的成分，包括 AEBSF、1,10-菲咯啉、Bestatin、E-64、亮肽素和 Pepstatin A，对半胱氨酸、丝氨酸、天冬氨酸、金属蛋白酶和氨肽酶具有广泛的特异性。这种蛋白酶抑制剂混合物已经针对植物细胞和组织提取物进行了优化和测试。这种蛋白酶抑制剂混合物以 DMSO 中的即用型溶液形式提供。
|
Ingredient |
Summary | CAS Number | Targets |
Smiles |
| AEBSF.HCl | 丝氨酸蛋白酶抑制剂 | 30827-99-7 | 蛋白酶|丝氨酸蛋白酶 | C1=CC(=CC=C1CCN)S(=O)(=O)F.Cl |
| 贝他汀 | 氨肽酶抑制剂 | 58970-76-6 | 蛋白酶|氨肽酶 | CC(C)CC(C(=O)O)NC(=O)C(C(CC1=CC=CC=C1)N)O |
| E-64 | 半胱氨酸蛋白酶抑制剂，不可逆 | 66701-25-5 | 蛋白酶|组织蛋白酶 | CC(C)CC(C(=O)NCCCCN=C(N)N)NC(=O)C1C(O1)C(=O)O |
| 亮肽素, 微生物 | 丝氨酸和半胱氨酸蛋白酶抑制剂 | 103476-89-7 | 蛋白酶|丝氨酸蛋白酶 | O=C(C(C([H])([H])C(C([H])([H])[H])([H])C([H])([ H])[H])([H])N([H])C(C([H])([H])[H])=O)N([H])C(C(N( [H])C(C([H])=O)([H])C([H])([H])C([H])([H])C([H])([ H])/N=C(N([H])[H])/N([H])[H])=O)([H])C([H])([H])C( C([H])([H])[H])([H])C([H])([H])[H] |
| 胃酶抑素A | 天冬氨酸蛋白酶抑制剂 | 26305-03-3 | 蛋白酶|其他蛋白酶 | CC(C)CC(C(CC(=O)O)O)NC(=O)C(C)NC(=O)CC(C(CC(C)C)NC(=O)C (C(C)C)NC(=O)C(C(C)C)NC(=O)CC(C)C)O |
| 邻菲咯啉 | 金属蛋白酶抑制剂 | 66-71-7 | 蛋白酶|其他蛋白酶 | C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3 |