PIPES
(Synonyms: 哌嗪-N,N'-二(2-乙磺酸),1,4-Piperazinediethanesulfonic acid) 目录号 : GC61192
PIPES(1,4-Piperazinediethanesulfonicacid)是PIPES缓冲液的重要组成部分，常用作生化试剂。
Cas No.:5625-37-6
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
PIPES (1,4-Piperazinediethanesulfonic acid) is an important component of PIPES buffer agent used in biochemistry[1].
To prepare the pH PIPES buffer, 173 g of 1,4-piperazinediethanesulfonic acid are dissolved into 1 L of deionized water. The pH of the PIPES buffer is adjusted to 6.8 by adding pellets of sodium hydroxide.PIPES buffer can be used to PIPES buffer, it can prevent the glutaraldehyde fixation induced lipid loss and artifacts[1].
[1]. Jason Moggridge, et al. Sensitive Detection of Immunoglobulin G Stability Using in Real-Time Isothermal Differential Scanning Fluorimetry: Determinants of Protein Stability for Antibody-Based Therapeutics. Technol Cancer Res Treat. 2017 Dec;16(6):997-1005.
| Cas No. | 5625-37-6 | SDF | |
| 别名 | 哌嗪-N,N'-二(2-乙磺酸),1,4-Piperazinediethanesulfonic acid | ||
| Canonical SMILES | O=S(CCN1CCN(CCS(=O)(O)=O)CC1)(O)=O | ||
| 分子式 | C8H18N2O6S2 | 分子量 | 302.37 |
| 溶解度 | Water: 5 mg/mL (16.54 mM; ultrasonic and adjust pH to 7 with NaOH) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.3072 mL | 16.536 mL | 33.0721 mL |
| 5 mM | 0.6614 mL | 3.3072 mL | 6.6144 mL |
| 10 mM | 0.3307 mL | 1.6536 mL | 3.3072 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Anisotropy in Polyurethane Pre-Insulated PIPES
Polymers (Basel) 2019 Dec 12;11(12):2074.PMID:31842365DOI:10.3390/polym11122074.
The polyurethane foam in district heating pre-insulated PIPES has a critical role to play both as thermal insulation and as load bearing element, as it serves as bond between the medium pipe and the casing. Hence, knowledge on how the foam behaves under multiaxial stresses is of great importance for the design as well as for aging predictions of the network. It is known that cell shape anisotropy in polymeric foams leads to anisotropy in its mechanical properties. In this study, we evaluate and quantify the microstructural anisotropy of PU foam from pre-insulated PIPES as well as its mechanical behaviour under compression in the three orthogonal directions. We cover rigid and flexible PU foam, batch and continuous manufacturing, and different pipe diameters. The results were compared with those predicted by available rectangular and Kelvin cell shape models. We have found that PU from pre-insulated PIPES is orthotropic and present stronger anisotropy than that typically found in PU slabs. The traditional bonded PIPES under consideration behaved in a similar way. However, when comparing the two flexible PIPES in this study, despite no significant differences in cell shape anisotropy were found, a significantly different behaviour for the E modulus ratio was observed. This shows that while the manufacturing process exerts the main influence on cell shape anisotropy, to explain the difference in stiffness behaviour other factors need to be taken into consideration, such as cell size and cell size variability.
An easy disinfection strategy for PIPES connecting hemodialysis equipment
Int J Artif Organs 2021 Jun;44(6):385-392.PMID:33222595DOI:10.1177/0391398820975034.
Introduction: A hemodialysis room has PIPES connecting the console and central fluid equipment. While these PIPES require disinfection, reports detailing disinfection strategies are unavailable. Therefore, we aimed to compare two easy disinfection strategies differing in sanitization frequency and sanitizer concentration. Methods: Reverse osmosis water (ROW) purifying equipment and six dialysis consoles were connected by 20 m of PIPES. Only ROW flowed through these PIPES, because the dialysate solution was constituted at each console. The PIPES were sanitized by two strategies: (1) strong and monthly (hypochlorite concentration: 100 ppm) or (2) weak and weekly (5 ppm). Both strategies were easy because the sodium hypochlorite was simply added to the ROW tank. To estimate sanitization efficacy, endotoxin counts at the ROW tank outlet, the end of the pipe, and the pipe after the endotoxin-cutting filter in each console were measured monthly for six continuous months. These counts were compared between the two sanitization strategies. Results: The endotoxin counts at the ROW tank outlet and the end of the pipe were 0.004-0.017 and 0.012-0.081 EU/mL, respectively, in the strong and monthly strategy, and 0.001-0.003 and 0.001-0.005 EU/mL, respectively, in the weak and weekly strategy. The endotoxin counts at the pipe after the endotoxin-cutting filter were less than 0.001 EU/mL during the study period in both strategies. Conclusion: A weekly disinfection strategy was more effective than a monthly one, despite the lower hypochlorite concentration. The present study suggests that frequency is the most important factor in the disinfection of PIPES in a dialysis room.
Evaluating the Residual Stress and Its Effect on the Quasi-Static Stress in Polyethylene PIPES
Polymers (Basel) 2022 Apr 3;14(7):1458.PMID:35406331DOI:10.3390/polym14071458.
Residual stress is generated during the production process. It can significantly affect the mechanical performance of pressurized polymer PIPES. In this paper, six polyethylene (PE) PIPES, including three high-density PEs (HDPE) and three medium-density PEs (MDPE) provided by different suppliers, were tested using a one-slit-ring method to measure the residual stress distribution along the hoop direction. Finite element (FE) simulation and mechanical testing were also employed in an iteration process to obtain the mechanical parameters of the six PE PIPES. For the same PE pipe code from different suppliers, the results show that the magnitude of the residual hoop stress can be very different, resulting in different mechanical behaviors. In addition, the results are proposed to explain the scenario that was reported previously, i.e., the different critical quasi-static stress (the time-independent stress) levels of the PE PIPES with the same pipe code. Since the quasi-static stress is expected to dominate the long-term behavior of the PE PIPES, it is of great importance to carefully consider the effect of the residual stress on the determination of the quasi-static stress.
A minimally designed soft crawling robot for robust locomotion in unstructured PIPES
Bioinspir Biomim 2022 Jul 8;17(5).PMID:35636388DOI:10.1088/1748-3190/ac7492.
Soft robots have attracted increasing attention due to their excellent versatility and broad applications. In this article, we present a minimally designed soft crawling robot (SCR) capable of robust locomotion in unstructured PIPES with various geometric/material properties and surface topology. In particular, the SCR can squeeze through narrow PIPES smaller than its cross section and propel robustly in spiked PIPES. The gait pattern and locomotion mechanism of this robot are experimentally investigated and analysed by the finite element analysis, revealing that the resultant forward frictional force is generated due to the asymmetric mechanical properties along the length direction of the robot. The proposed simple yet working SCR could inspire novel designs and applications of soft robots in unstructured narrow canals such as large intestines or industrial pipelines.
Application of Additively Manufactured Pentamode Metamaterials in Sodium/Inconel 718 Heat PIPES
Materials (Basel) 2021 Jun 2;14(11):3016.PMID:34199372DOI:10.3390/ma14113016.
In this study, pentamode metamaterials were proposed for thermal stress accommodation of alkali metal heat PIPES. Sodium/Inconel 718 heat PIPES with and without pentamode metamaterial reinforcement were designed and fabricated. Then, these heat PIPES were characterized by startup tests and thermal response simulations. It was found that pentamode metamaterial reinforcement did not affect the startup properties of sodium/Inconel 718 heat PIPES. At 650-950 °C heating, there was a successful startup of heat PIPES with and without pentamode metamaterial reinforcement, displaying uniform temperature distributions. A further simulation indicated that pentamode metamaterials could accommodate thermal stresses in sodium/Inconel 718 heat PIPES. With pentamode metamaterial reinforcement, stresses in the heat PIPES decreased from 12.9-62.1 to 10.2-52.4 MPa. As a result, sodium/Inconel 718 heat PIPES could be used more confidently. This work was instructive for the engineering application of alkali metal heat PIPES.