PDE3A Protein, Human (sf9, His-GST)
目录号 : GC27239PDE3A 蛋白是一种环核苷酸磷酸二酯酶，靶向 cAMP、cGMP 和 cUMP，这些在多种生理过程中至关重要。在 E2/17β-雌二醇诱导的促凋亡途径中，E2 稳定 PDE3A/SLFN12 复合物，导致 SLFN12 去磷酸化并激活其促凋亡核糖核酸酶活性。该途径与 E2 浓度高的组织有关，可能有助于胎盘重塑等过程。PDE3A 蛋白, Human (sf9, His-GST) 是重组的 PDE3A 蛋白，由 Sf9 insect cells 表达，带有 N-His, N-GST 标签。
Sample solution is provided at 25 µL, 10mM.
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PDE3A protein, a cyclic nucleotide phosphodiesterase, targets cAMP, cGMP, and cUMP, crucial in diverse physiological processes. In an E2/17beta-estradiol-induced pro-apoptotic pathway, E2 stabilizes the PDE3A/SLFN12 complex, leading to SLFN12 dephosphorylation and activating its pro-apoptotic ribonuclease activity. This pathway is implicated in tissues with high E2 concentrations, potentially contributing to processes like placenta remodeling. PDE3A Protein, Human (sf9, His-GST) is the recombinant human-derived PDE3A protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
| Purity | Greater than 90% as determined by reducing SDS-PAGE | Source | Sf9 insect cells |
| Phycical Appearance | Solution. | Shipping Condition | Shipping with dry ice. |
| Stability | Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles. | ||
| Biological Activity | The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | N/A. | ||
| Formulation | Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10% glycerol, 3 mM DTT. | ||
PDE3A 蛋白是一种环核苷酸磷酸二酯酶，对第二信使 cAMP 和 cGMP 表现出特异性，而 cAMP 和 cGMP 是多种生理过程中的关键调节因子。此外，PDE3A 还表现出对 cUMP 的活性。除了其催化作用之外，PDE3A 也是 E2/17β-雌二醇诱导的促凋亡信号通路的一个组成部分。雌二醇 (E2) 可稳定细胞质中的 PDE3A/SLFN12 复合物，导致 SLFN12 去磷酸化并激活其促凋亡核糖核酸酶活性。这种细胞凋亡途径与高浓度 E2 的组织有关，并且可能在胎盘重塑等过程中发挥作用。
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.