PBFI AM
(Synonyms: Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester) 目录号 : GC18477A cell-permeable, potassium-sensitive fluorophore
Cas No.:124549-23-1
Sample solution is provided at 25 µL, 10mM.
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
PBFI AM is a cell-permeable, potassium-sensitive fluorophore used to measure potassium changes in cells and intracellular compartments.[1] [2] As PBFI AM enters cells, it is hydrolyzed by intracellular esterases to produce PBFI. The affinity of PBFI for potassium is sodium-dependent (Kd = 44 and 5.1 mM with or without sodium, respectively). [3] Absorption and emission maxima are 336 and 480-557 nm, respectively.[4] [5]
Reference:
[1]. Grieshaber, S., Swanson, J.A., and Hackstadt, T. Determination of the physical environment within the Chlamydia trachomatis inclusion using ion-selective ratiometric probes. Cell. Microbiol. 4(5), 273-283 (2002).
[2]. Ljubkovic, M., Marinovic, J., Fuchs, A., et al. Targeted expression of Kir6.2 in mitochondria confers protection against hypoxic stress. J. Physiol. 577(Pt 1), 17-29 (2006).
[3]. Halperin, S.J., and Lynch, J.P. Effects of salinity on cytosolic Na+ and K+ in root hairs of Arabidopsis thaliana: In vivo measurements using the fluorescent dyes SBFI and PBFI. J. Exp. Bot. 54(390), 2035-2043 (2003).
[4]. Sabnis, R.W. Handbook of biological dyes and stains: Synthesis and industrial applications. (2010).
[5]. Jezek, P., Mahdi, F., and Garlid, K.D. Reconstitution of the beef heart and rat liver mitochondrial K+/H+ (Na+/H+) antiporter. Quantitation of K+ transport with the novel fluorescent probe, PBFI. J. Biol. Chem. 265(18), 10522-10526 (1990).
| Cas No. | 124549-23-1 | SDF | |
| 别名 | Potassium-binding Benzofuran Isophthalate Acetoxymethyl ester | ||
| 化学名 | 4,4'-[1,4,10,13-tetraoxa-7,16-diazacyclooctadecane-7,16-diylbis(5-methoxy-6,2-benzofurandiyl)]bis-1,3-benzenedicarboxylic acid, tetrakis[(acetyloxy)methyl] ester | ||
| Canonical SMILES | COC1=CC2=C(OC(C3=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C3)=C2)C=C1N4CCOCCOCCN(C5=CC(OC(C6=C(C(OCOC(C)=O)=O)C=C(C(OCOC(C)=O)=O)C=C6)=C7)=C7C=C5OC)CCOCCOCC4 | ||
| 分子式 | C58H62N2O24 | 分子量 | 1171.1 |
| 溶解度 | Soluble in methanol and DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 0.8539 mL | 4.2695 mL | 8.539 mL |
| 5 mM | 0.1708 mL | 0.8539 mL | 1.7078 mL |
| 10 mM | 0.0854 mL | 0.4269 mL | 0.8539 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。