PBD-BODIPY
目录号 : GC44574
A probe for autoxidation reactions
Cas No.:148185-52-8
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
本方案仅提供一个指导，请根据您的具体需要进行修改。
PBD-BODIPY是用于自动氧化反应的分光光度测量的探针。
应用案例：PBD-BODIPY在苯乙烯自氧化中作为信号载体
文献来源：
Omkar Zilka, et al.On the Mechanism of Cytoprotection by Ferrostatin-1 and Liproxstatin-1 and the Role of Lipid Peroxidation in Ferroptotic Cell Death. ACS Cent Sci. 2017 Mar 22;3(3):232-243. doi: 10.1021/acscentsci.7b00028. Epub 2017 Mar 7.
实验方法：
1．苯乙烯用1 M NaOH水溶液洗涤三次，用MgSO4干燥，过滤，真空蒸馏，并通过二氧化硅，然后碱性氧化铝的沉淀纯化；
2 . 向含1.25 mL苯乙烯的比色皿中加入1.18 mL氯苯，并将溶液在37℃下平衡5 min；
3．将比色皿空白，加入12.5 μL 2 mM PBD-BODIPY的1，2，4-三氯苯溶液，然后加入50 μL 0.3 M AIBN的氯苯溶液，并充分混合溶液；
4．20分钟后，加入Lip-1、Fer-1、C15-THN、PMHC或α-TOH的氯苯储备溶液（1 mM），然后在591 nm处丧失的吸光度。PBD-BODIPY 信号载体和碳氢化合物共底物的共自氧化可以通过检测591 nm处吸光度的损失来量化。
实验结果示例：
PBD-BODIPY serves as the signal carrier in styrene autoxidations (A), enabling determination of rate constants (kinh) and stoichiometries (n) for reactions of inhibitors with chain-carrying peroxyl radicals (B). Coautoxidations of styrene (4.3 M) and PBD-BODIPY (10 μM) initiated by AIBN (6 mM) in chlorobenzene at 37 °C (black trace) and inhibited by 2 μM (red trace), 3 μM (green trace), and 4 μM (blue trace) of Fer-1 (C), Lip-1 (D), C15-THN (E), α-TOH (F), and PMHC (G). Average inhibition rate constants and stoichiometry summarized in panel H. Reaction progress was monitored by absorbance at 591 nm (ε = 139,000 M–1 cm–1).
PBD-BODIPY 充当苯乙烯自氧化 (A) 中的信号载体，能够确定抑制剂与链携带过氧自由基 (B) 反应的速率常数 (k inh ) 和化学计量 (n)。 苯乙烯 (4.3 M) 和 PBD-BODIPY (10 μM) 的共氧化反应由 AIBN (6 mM) 在氯苯中于 37 °C（黑色迹线）引发，并被 2 μM（红色迹线）、3 μM（绿色迹线）和 4 μM（蓝色迹线）的 Fer-1 (C)、Lip-1 (D)、C15-THN (E)、α-TOH (F) 和 PMHC (G)。 平均抑制率常数和化学计量总结于图H中。通过591 nm (ε = 139,000 M-1 cm-1)处的吸光度监测反应进程。
注意事项：
1）荧光染料均存在淬灭问题，请尽量注意避光，以减缓荧光淬灭。
2）为了您的安全和健康，请穿实验服并戴一次性手套操作。
PBD-BODIPY is a probe for the spectrophotometric measurement of autoxidation reactions. Co-autoxidation of the PBD-BODIPY signal carrier and a hydrocarbon co-substrate can be quantified by monitoring loss of absorbance at 591 nm. PBD-BODIPY has been used to measure the activity of radical-trapping antioxidants in cell-free assays. It has also been used as a fluorescent probe for the detection of epoxidation activity.
| Cas No. | 148185-52-8 | SDF | |
| Canonical SMILES | [F][B-]([N]1C2=CC=C1/C=C/C=C/C3=CC=CC=C3)([N+](C(C(C)=C4)=C2)=C4C)[F] | ||
| 分子式 | C21H19BF2N2 | 分子量 | 348.2 |
| 溶解度 | Benzene: 1 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.8719 mL | 14.3596 mL | 28.7191 mL |
| 5 mM | 0.5744 mL | 2.8719 mL | 5.7438 mL |
| 10 mM | 0.2872 mL | 1.436 mL | 2.8719 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
A Continuous Visible Light Spectrophotometric Approach To Accurately Determine the Reactivity of Radical-Trapping Antioxidants
J Org Chem 2016 Feb 5;81(3):737-44.PMID:26529543DOI:10.1021/acs.joc.5b02183
Inhibited autoxidations-monitored either by O2 consumption or hydroperoxide formation-are the most reliable way to obtain kinetic and stoichiometric information on the activity of radical-trapping antioxidants (RTAs). While many comparatively simple "antioxidant assays" (e.g., the DPPH assay) have supplanted these in popularity, they are generally very poor substitutes since they often do not employ peroxyl radicals as the oxidant and do not account for both the kinetics and stoichiometry of the radical-trapping reaction(s). In an effort to make inhibited autoxidations as simple as the most popular "antioxidant assays", we have developed a spectrophotometric approach for monitoring reaction progress in inhibited autoxidations. The approach employs easily prepared 1-phenylbutadiene-conjugated or styrene-conjugated BODIPY chromophores (PBD-BODIPY or STY-BODIPY, respectively) as signal carriers that co-autoxidize along with a hydrocarbon substrate. We show that inhibition rate constants (kinh) are accurately determined for a range of phenolic and diarylamine RTAs using this approach and that mechanistic experiments, such as kinetic isotope effects and kinetic solvent effects, are equally easily carried out. Moreover, synergistic interactions between RTAs, as well as the unconventional activity of diarylamine RTAs, are captured using this methodology. Lastly, we show that the approach can be employed for monitoring reactions in aqueous solution.