o-Phenanthroline
(Synonyms: 1,10-菲罗啉) 目录号 : GC10156
o-Phenanthroline是一种常用的有机螯合剂和配体，能与多种金属离子（如Fe²⁺、Zn²⁺）形成稳定的配合物。
Cas No.:66-71-7
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
o-Phenanthroline is a commonly used organic chelating agent and ligand that can form stable complexes with a variety of metal ions (such as Fe²⁺, Zn²⁺) [1]. o-Phenanthroline forms a red-orange complex with Fe²⁺, with a maximum absorption wavelength of about 510nm [2]. o-Phenanthroline is also an important broad-spectrum inhibitor of matrix metalloproteinases (MMPs). Its mechanism of action is to inhibit the enzymatic activity of MMP by chelating the metal ions (such as Zn²⁺) in the active center of MMP [3-4].
In human umbilical vein endothelial cells o-Phenanthroline (0.3mM; 24h) had some cytotoxicity to the cells, and the level of MMP-2 detected in gelatin zymography was decreased [5]. In cultured endothelial cells exposed to the enzyme reaction hypoxanthine-xanthine oxidase (HX-XO) or pure H2O2, O-phenanthroline (100μM; 15min) afforded a complete protective effect against DNA strand breakage and the associated activation of the enzyme poly(ADP) ribose polymerase [6]. In Human primary chondrocytes, the upregulation of HIF1α expression in cells treated with O-Phenanthroline (2-5μM; 10h) [7].
In BALB/c mice, hematopoietic stem/progenitor cells mobilization was significantly inhibited by o-Phenanthroline (100mg/kg; ip; 3d) [6].
References:
[1]. Mu G N, Li X, Li F. Synergistic inhibition between o-phenanthroline and chloride ion on cold rolled steel corrosion in phosphoric acid[J]. Materials Chemistry and Physics, 2004, 86(1): 59-68.
[2]. Liu C, Liu Q, Lv Y, et al. CPLX1 is a novel prognostic biomarker in CRC correlating with immunotherapy resistance and ferroptosis[J]. Frontiers in Immunology, 2025, 16: 1589423.
[3]. Liu ZiQiang, Liu YuXin, Zhou DaYong, et al. The role of matrix metalloprotease (MMP) to the autolysis of sea cucumber (Stichopus japonicus)[J]. 2019.
[4]. Kato Y, Yamashita T, Ishikawa M. Relationship between expression of matrix metalloproteinase-2 and matrix metalloproteinase-9 and invasion ability of cervical cancer cells[J]. Oncology reports, 2002, 9(3): 565-569.
[5]. Lafleur M A, Hollenberg M D, Atkinson S J, et al. Activation of pro-(matrix metalloproteinase-2)(pro-MMP-2) by thrombin is membrane-type-MMP-dependent in human umbilical vein endothelial cells and generates a distinct 63 kDa active species[J]. Biochemical Journal, 2001, 357(1): 107-115.
[6]. Jin F, Zhai Q, Qiu L, et al. Degradation of BM SDF-1 by MMP-9: the role in G-CSF-induced hematopoietic stem/progenitor cell mobilization[J]. Bone marrow transplantation, 2008, 42(9): 581-588.
[7]. Georgi N, Landman E B M, Klein T J, et al. O‐Phenanthroline as modulator of the hypoxic and catabolic response in cartilage tissue‐engineering models[J]. Journal of tissue engineering and regenerative medicine, 2017, 11(3): 724-732.
o-Phenanthroline是一种常用的有机螯合剂和配体，能与多种金属离子（如Fe²⁺、Zn²⁺）形成稳定的配合物 [1]。o-Phenanthroline与Fe²⁺形成橙红色配合物，最大吸收波长约为510nm [2]。o-Phenanthroline也是一种重要的基质金属蛋白酶（MMP）广谱抑制剂，其作用机制是通过螯合MMP活性中心的金属离子（如Zn²⁺）来抑制MMP的酶活性 [3-4]。
在人脐静脉内皮细胞中，o-Phenanthroline（0.3mM；24h）对细胞有一定的细胞毒性，明胶酶谱法检测的MMP-2水平降低 [5]。在培养的内皮细胞中，暴露于次黄嘌呤-黄嘌呤氧化酶（HX-XO）或纯H2O2的酶反应中，o-Phenanthroline（100μM；15min）可完全保护内皮细胞免受DNA链断裂及其相关的聚（ADP）核糖聚合酶的激活 [6]。在人原代软骨细胞中，用o-Phenanthroline（2-5μM；10h）处理的细胞中HIF1α表达上调 [7]。
在BALB/c小鼠中，c（100mg/kg；ip；3d）显著抑制造血干细胞/祖细胞的动员 [6]。
| Cell experiment [1]: | |
Cell lines | Human umbilical vein endothelial cells (HUVECs) |
Preparation Method | HUVECs were cultured on type I collagen-coated plates using serum-free medium, and multiple protease inhibitors including o-Phenanthroline (0.3mM) were added to the experimental treatment groups. o-Phenanthroline is a broad-spectrum inhibitor of metalloproteinases (such as MMPs) that inhibits MMP activity by chelating metal ions. After o-phenanthroline treatment, thrombin was also added to the experimental group of cells and incubated at 37℃ for 24 hours. |
Reaction Conditions | 0.3mM; 24h |
Applications | In HUVECs, o-Phenanthroline had some cytotoxicity to the cells, and the level of MMP-2 detected in gelatin zymography was decreased. |
| Animal experiment [2]: | |
Animal models | BALB/c mice |
Preparation Method | BALB/c mice (6- to 8-week old) were divided into four groups (three mice per group). The first group received PBS for 5 days. The second group was treated by once daily s.c. injection of G-CSF at a dose of 250mg/kg body weight (b.w.) per day for 5 consecutive days. The third group was administered i.p. injection of SDF-1 MoAb at 100mg/kg b.w. per day on days 4 and 5 in combination with G-CSF 250mg/kg b.w. per day. The fourth group was administered i.p. injections of MMP-9 inhibitor o-Phenanthroline at 100mg/kg b.w. per day on days 2 and 5 in combination with G-CSF 250mg/kg b.w per day. |
Dosage form | 100mg/kg; ip; 3d |
Applications | In BALB/c mice, hematopoietic stem/progenitor cells mobilization was significantly inhibited by o-Phenanthroline. |
References: | |
| Cas No. | 66-71-7 | SDF | |
| 别名 | 1,10-菲罗啉 | ||
| 化学名 | 1,10-phenanthroline | ||
| Canonical SMILES | C(C=CC=N1)(C=C2)=C1C3=C2C=CC=N3 | ||
| 分子式 | C12H8N2 | 分子量 | 180.21 |
| 溶解度 | ≥ 360.42mg/mL in DMSO | 储存条件 | Store at 4°C, protect from light, stored under nitrogen |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 5.5491 mL | 27.7454 mL | 55.4908 mL |
| 5 mM | 1.1098 mL | 5.5491 mL | 11.0982 mL |
| 10 mM | 0.5549 mL | 2.7745 mL | 5.5491 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet