NLRP3 Protein, Human (His)
目录号 : GP27283NLRP3 蛋白充当 NLRP3 炎症小体中的传感器，该传感器响应膜缺陷而被激活。它与CASP1和PYCARD/ASC形成复合物，促进IL1B、IL18和GSDMD的释放，并诱导细胞焦亡。NLRP3 蛋白, Human (His) 是重组的 NLRP3 蛋白，由 E. coli 表达，带有 C-6*His 标签。
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
The NLRP3 protein acts as a sensor in the NLRP3 inflammasome, which is activated in response to membrane defects. It forms a complex with CASP1 and PYCARD/ASC, promotes the release of IL1B, IL18 and GSDMD, and induces pyroptosis. NLRP3 Protein, Human (His)
NLRP3 蛋白作为 NLRP3 炎症小体的传感器组件，协调炎症小体的激活，以响应损害膜完整性的各种刺激，导致炎症细胞因子 IL1B 和 IL18 的分泌以及细胞焦亡。在存在影响膜完整性的病原体或损伤相关信号的情况下，NLRP3 启动炎性体复合物的组装，该复合物包括 NLRP3、CASP1 和 PYCARD/ASC。这导致 caspase-1 前体的募集和激活，随后裂解并激活 IL1B、IL18 和 Gasdermin-D (GSDMD)，促进细胞因子释放和焦亡细胞死亡。激活刺激包括细胞外ATP、尼日利亚菌素、活性氧、晶体、β-淀粉样蛋白纤维和各种颗粒或纳米颗粒。有趣的是，NLRP3 还在调节辅助性 T 2 (Th2) 细胞的分化中发挥作用，有助于 Th2 细胞依赖性过程，例如哮喘和肿瘤生长。在 Th2 分化过程中，NLRP3 参与 IRF4 与 IL4 启动子的最佳结合以及 IRF4 依赖性 IL4 转录，突出了其除炎症体激活之外的多方面功能。 is the recombinant human-derived NLRP3, expressed by E. coli, with C-6*His labeled tag.
| Purity | Greater than 90% as determined by reducing SDS-PAGE. | Source | E. coli |
| Phycical Appearance | Lyophilized powder | Shipping Condition | Room temperature in continental US; may vary elsewhere. |
| Synonyms | Angiotensin/vasopressin receptor AII/AVP-like; Caterpiller protein 1.1; Cold-induced autoinflammatory syndrome 1 protein; Cryopyrin; PYRIN-containing APAF1-like protein 1; NLRP3; NALP3; PYPAF1; CIAS1 | ||
| Apparent Molecular Weight | Approximately 72 kDa | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.22 μm filtered solution of PBS, 6% Trehalose, pH 7.4 or 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0. Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips | ||
The NLRP3 protein acts as a sensor in the NLRP3 inflammasome, which is activated in response to membrane defects. It forms a complex with CASP1 and PYCARD/ASC, promotes the release of IL1B, IL18 and GSDMD, and induces pyroptosis. NLRP3 Protein, Human (His) is the recombinant human-derived NLRP3, expressed by E. coli, with C-6*His labeled tag.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.