N,N'-Dimethylthiourea
(Synonyms: N,N-二甲基硫脲; DMTU) 目录号 : GC61644
N,N'-Dimethylthiourea(DMTU)是从黑蒜中分离出来的，是口服有效的羟基自由基(•OH)的清除剂，通过活化的中性粒细胞在体外阻止•OH的产生。N,N'-Dimethylthiourea通过发挥抗氧化作用，包括•OH清除和抗炎作用，防止水浸限制应激(WIRS)诱导的大鼠胃粘膜损伤。
Cas No.:534-13-4
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
N,N'-Dimethylthiourea (DMTU), isolated from Allii Sativi Bulbus, is an orally active scavenger of hydroxyl radical (•OH) and blocks •OH production by activated neutrophils in vitro. N,N'-Dimethylthiourea protects against water-immersion restraint stress (WIRS)-induced gastric mucosal lesions in rats by exerting its antioxidant action including •OH scavenging and anti-inflammatory action[1][2].
N,N'-Dimethylthiourea (DMTU; 1, 2.5, or 5 mmol/kg; orally) administered at 0.5 h before the onset of WIRS reduces the severity of gastric mucosal lesions with attenuation of the changes in the levels of gastric mucosal MPO, pro-inflammatory cytokines, LPO, NOx, nonprotein SH, and vitamin C and gastric adherent mucus found at 3 h after the onset of WIRS in a dose-dependent manner in Male Wistar rats aged 6 weeks[1].
[1]. Yoshiji Ohta, et al. Protective effect of N,N'-dimethylthiourea against stress-induced gastric mucosal lesions in rats. Fundam Clin Pharmacol. 2017 Jun;31(3):319-328. [2]. Zhongyi Zhang, et al. Analysis of volatile compounds in fermented black garlic by GC-MS.
| Cas No. | 534-13-4 | SDF | |
| 别名 | N,N-二甲基硫脲; DMTU | ||
| Canonical SMILES | S=C(NC)NC | ||
| 分子式 | C3H8N2S | 分子量 | 104.17 |
| 溶解度 | 储存条件 | 4°C, stored under nitrogen | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 9.5997 mL | 47.9985 mL | 95.9969 mL |
| 5 mM | 1.9199 mL | 9.5997 mL | 19.1994 mL |
| 10 mM | 0.96 mL | 4.7998 mL | 9.5997 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Protective effect of N,N'-Dimethylthiourea against stress-induced gastric mucosal lesions in rats
Fundam Clin Pharmacol 2017 Jun;31(3):319-328.PMID:28107553DOI:10.1111/fcp.12268.
In the present study, we examined the protective effect of N,N'-Dimethylthiourea (DMTU), a scavenger of hydroxyl radical (·OH), against water-immersion restraint stress (WIRS)-induced gastric mucosal lesions in rats. When male Wistar rats fasted for 24 h were exposed to WIRS for 3 h, gastric mucosal lesions occurred with increases in the levels of gastric mucosal myeloperoxidase (MPO), an index of tissue neutrophil infiltration, pro-inflammatory cytokines (tumor necrosis factor alpha and interleukin 1beta), lipid peroxide (LPO), and nitrite/nitrate (NOx), an index of nitric oxide synthesis, and decreases in the levels of gastric mucosal nonprotein SH and vitamin C and gastric adherent mucus. DMTU (1, 2.5, or 5 mmol/kg) administered orally at 0.5 h before the onset of WIRS reduced the severity of gastric mucosal lesions with attenuation of the changes in the levels of gastric mucosal MPO, pro-inflammatory cytokines, LPO, NOx, nonprotein SH, and vitamin C and gastric adherent mucus found at 3 h after the onset of WIRS in a dose-dependent manner. Serum levels of corticosterone and glucose, which are indices of stress responses, increased in rats exposed to WIRS for 3 h, but DMTU pre-administered at any dose had no effect on these increases. These results indicate that DMTU protects against WIRS-induced gastric mucosal lesions in rats by exerting its antioxidant action including ·OH scavenging and its anti-inflammatory action without affecting the stress response.
N,N'-Dimethylthiourea dioxide formation from N,N'-Dimethylthiourea reflects hydrogen peroxide concentrations in simple biological systems
Proc Natl Acad Sci U S A 1988 May;85(10):3422-5.PMID:3130627DOI:10.1073/pnas.85.10.3422.
We hypothesized that measurement of a specific product from reaction of N,N'-Dimethylthiourea (Me2TU) and H2O2 would provide a good indication of the H2O2 scavenging and protection seen after addition of Me2TU to biological systems. We found that addition of H2O2 to Me2TU yielded a single stable product, Me2TU dioxide. Me2TU dioxide formation correlated with Me2TU consumption as a function of added H2O2 concentration and was prevented by simultaneous addition of catalase (but not boiled catalase), superoxide dismutase, dimethyl sulfoxide, mannitol, or sodium benzoate. Me2TU dioxide formation, Me2TU consumption, and H2O2 concentration increases occurred in mixtures containing phorbol 12-myristate 13-acetate (PMA) and normal human neutrophils but not in mixtures containing PMA and neutrophils from patients with chronic granulomatous disease or in mixtures containing PMA and normal neutrophils and catalase. Me2TU dioxide formation also occurred in isolated rat lungs perfused with Me2TU and H2O2 but not in lungs perfused with Me2TU and elastase, histamine, or oleic acid. In contrast, Me2TU dioxide formation did not occur after exposure of Me2TU to 60Co-generated hydroxyl radical or hypochlorous acid in the presence of catalase. The results indicate that reaction of Me2TU with H2O2 selectively forms Me2TU dioxide and that measuring Me2TU dioxide formation from Me2TU may be useful for assessing the presence and significance of H2O2 in biological systems.
Role of chelate substituents and cis sigma-effect on the rate of ligand substitution at Pt(N-N-N) and Pt(N-N-C) centres
Dalton Trans 2004 Jan 21;(2):299-304.PMID:15356727DOI:10.1039/b311595j.
Four complexes of the type [Pt(N-N-X)Cl](X = N or C) were tailor synthesized for mechanistic studies in methanol. The terdentate ligands included terpy, 4'-Ph-terpy, 4'-(2"-CF(3)-Ph)-terpy, and 4'-(2"'-CF(3)-Ph)-6-Ph-2,2'-bipy. The rate of substitution of the chloro ligand by thiourea, N,N'-Dimethylthiourea, and N,N,N',N'-tetramethylthiourea was studied as a function of nucleophile concentration, temperature and pressure by using a stopped-flow technique. The observed pseudo-first-order rate constants for the substitution reactions obeyed the simple rate law k(obs) = k(2)[Nu]. Second-order kinetics and negative activation entropies and volumes support an associative substitution mechanism. At 298 K, the values of the second-order rate constant show a slight dependence on the nature of the moiety attached to the terpy ligand. Changing from a nitrogen sigma-donor to a carbon sigma-donor in the cis position, results in a deceleration of the substitution rate. The results suggest that the Pt-C bond in the cis position activates the metal centre in a different way than in the trans position.