n-Dodecyl-β-D-maltoside
(Synonyms: 十二烷基-β-D-麦芽糖苷) 目录号 : GC44357
n-Dodecyl β-D-maltoside 作为一种非离子洗涤剂，具有温和和非变性的特性，可用于蛋白质麻醉研究。
Cas No.:69227-93-6
Sample solution is provided at 25 µL, 10mM.
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n-Dodecyl β-D-maltoside, as a non-ionic detergent, has mild and non-denaturing properties used for during protein-anesthetic studies.[1]
In vitro, n-Dodecyl β-D-maltoside specifically interacts with HSA with Kd of 40 μM with a lower affinity than SDS with Kd of 2 μM. [1] In vitro efficacy test it shown that the magnitude of interactions between n-dodecyl-beta-D-maltoside and other surfactants followed the order anionic/nonionic > cationic/nonionic > nonionic/nonionic mixtures. In addition, electrolyte reduced synergism between n-dodecyl-beta-D-maltoside and ionic surfactant due to charge neutralization.[2] In vitro, after exposure to 2 mM n-Dodecyl β-D-maltoside, enterocytes were also generally well preserved, n-Dodecyl β-D-maltoside caused a obvious shortening of the microvilli of villus enterocytes compared with NaC.[3]
References:
[1]Xu L, et al. n-Dodecyl β-D-maltoside specifically competes with general anesthetics for anesthetic binding sites. J Biomol Struct Dyn. 2014;32(11):1833-40.
[2]Zhang R, et al. Study of mixtures of n-dodecyl-beta-D-maltoside with anionic, cationic, and nonionic surfactant in aqueous solutions using surface tension and fluorescence techniques. J Colloid Interface Sci. 2004 Oct 15;278(2):453-60.?
[3]Danielsen EM, et al. Probing the Action of Permeation Enhancers Sodium Cholate and N-dodecyl-β-D-maltoside in a Porcine Jejunal Mucosal Explant System. Pharmaceutics. 2018 Oct 2;10(4):172.?
n-Dodecyl β-D-maltoside 作为一种非离子洗涤剂,具有温和和非变性的特性,可用于蛋白质麻醉研究。[1]
在体外,正十二烷基 β-D-麦芽糖苷与 HSA 特异性相互作用,Kd 为 40 μM,亲和力低于 SDS,Kd 为 2 μM。 [1] 体外药效试验表明正十二烷基-β-D-麦芽糖苷与其他表面活性剂相互作用的大小顺序为阴离子/非离子>阳离子/非离子 >非离子/非离子混合物。此外,由于电荷中和,电解质降低了正十二烷基-β-D-麦芽糖苷和离子表面活性剂之间的协同作用。[2] 在体外,暴露于 2 mM 正十二烷基 β-D-麦芽糖苷后,肠细胞也普遍保存完好,与NaC相比,正十二烷基β-D-麦芽糖苷导致绒毛肠细胞的微绒毛明显缩短。[3]
| Cell experiment [1]: | |
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Cell lines |
Organ Culture of Porcine Jejunal Mucosa |
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Preparation Method |
The dishes were placed in an incubator kept at 37 °C, and after 15 min of preincubation, reagents were added to the RPMI medium at the following concentrations: NaC and n-Dodecyl-β-D-maltoside (2 mM or 10 mM), LY (0.5 mg/mL), FM dye (10 µg/mL), and TRD (50 µg/mL). The explants were cultured in the presence of the reagents for 1 h in the dark, and after culture they were carefully rinsed in fresh RPMI medium and immersed in a fixative overnight at 4 °C. |
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Reaction Conditions |
2 mM or 10 mM; for 1 h in the dark |
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Applications |
Exposure to either NaC or n-Dodecyl-β-D-maltoside at a concentration of 2 mM for 1 h caused no gross overall deterioration of the mucosal morphology. In contrast, at a concentration of 10 mM, both PEs caused extensive denudation at the tip of the villi, which are the most exposed and sensitive areas of the mucosal epithelium. |
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References: [1]. Danielsen EM, et al. Probing the Action of Permeation Enhancers Sodium Cholate and N-dodecyl-β-D-maltoside in a Porcine Jejunal Mucosal Explant System. Pharmaceutics. 2018 Oct 2;10(4):172. |
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| Cas No. | 69227-93-6 | SDF | |
| 别名 | 十二烷基-β-D-麦芽糖苷 | ||
| Canonical SMILES | OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)[C@](O[C@H]2[C@H](O)[C@@H](O)[C@H](OCCCCCCCCCCCC)O[C@@H]2CO)([H])O1 | ||
| 分子式 | C24H46O11 | 分子量 | 510.6 |
| 溶解度 | DMF: 20 mg/ml,DMSO: 10 mg/ml,Ethanol: 10 mg/ml,PBS (pH 7.2): 2 mg/ml | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.9585 mL | 9.7924 mL | 19.5848 mL |
| 5 mM | 0.3917 mL | 1.9585 mL | 3.917 mL |
| 10 mM | 0.1958 mL | 0.9792 mL | 1.9585 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet