LSD1 Protein, Human (sf9, His-GST)
目录号 : GC27195LSD1 蛋白是一种组蛋白去甲基化酶，通过对“Lys-4”(H3K4me) 和“Lys-9”(H3K9me) 去甲基化来修饰组蛋白 H3。它充当共激活剂或辅阻遏物，通过 FAD 氧化底物，生成随后水解的亚胺。LSD1 蛋白, Human (sf9, His-GST) 是重组的 LSD1 蛋白，由 Sf9 insect cells 表达，带有 N-His, N-GST 标签。
Sample solution is provided at 25 µL, 10mM.
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LSD1 蛋白是一种组蛋白去甲基化酶，通过对“Lys-4”(H3K4me) 和“Lys-9”(H3K9me) 去甲基化来修饰组蛋白 H3。它充当共激活剂或辅阻遏物，通过 FAD 氧化底物，生成随后水解的亚胺。LSD1 蛋白, Human (sf9, His-GST) 是重组的 LSD1 蛋白，由 Sf9 insect cells 表达，带有 N-His, N-GST 标签。
LSD1 是一种组蛋白去甲基化酶，具有使组蛋白 H3 的“Lys-4”(H3K4me) 和“Lys-9”(H3K9me) 去甲基化的独特能力，根据细胞环境充当多功能共激活子或辅阻遏物。通过 FAD 介导的氧化和随后的亚胺水解，LSD1 可以使单甲基化和二甲基化的 H3K4me 去甲基化，从而有助于表观遗传转录激活的调节。在缺乏 RCOR1/CoREST 的情况下，单独使用 LSD1 无法使核小体上的 H3K4me 去甲基化。此外，LSD1 作为雄激素受体 (AR) 依赖性转录的共激活剂，参与 H3K9me（与表观遗传转录抑制相关的标记物）的去甲基化。此外，LSD1 在抑制神经元基因中发挥着关键作用，并通过参与 RCOR/GFI/KDM1A/HDAC 复合物协调 E-钙粘蛋白/CDH1、CDN7 和 KRT8 的抑制。除了组蛋白去甲基化之外，LSD1 的多种相互作用还延伸到 p53/TP53 去甲基化、稳定 DNMT1 和修饰 SOX2。LSD1 的复杂调节作用涵盖胚胎发生、原肠胚形成和多谱系血细胞发育，强调其在塑造表观遗传景观和影响各种细胞过程中的重要性。
| Purity | Greater than 90% as determined by reducing SDS-PAGE. | Source | Sf9 insect cells |
| Phycical Appearance | Lyophilized powder. | Shipping Condition | |
| Synonyms | LSD1 蛋白; Lysine-specific histone demethylase 1A; KDM1A; AOF2; KDM1; KIAA0601; LSD1 | ||
| Amino Acid Sequence | SGVEGAAFQSRLPHDRMTSQEAACFPDIISGPQQTQKVFLFIRNRTLQLWLDNPKIQLTFEATLQQLEAPYNSDTVLVHRVHSYLERHGLINFGIYKRIKPLPTKKTGKVIIIGSGVSGLAAARQLQSFGMDVTLLEARDRVGGRVATFRKGNYVADLGAMVVTGLGGNPMAVVSKQVNMELAKIKQKCPLYEANGQAVPKEKDEMVEQEFNRLLEATSYLSHQLDFNVLNNKPVSLGQALEVVIQLQEKHVKDEQIEHWKKIVKTQEELKELLNKMVNLKEKIKELHQQYKEASEVKPPRDITAEFLVKSKHRDLTALCKEYDELAETQGKLEEKLQELEANPPSDVYLSSRDRQILDWHFANLEFANATPLSTLSLKHWDQDDDFEFTGSHLTVRNGYSCVPVALAEGLDIKLNTAVRQVRYTASGCEVIAVNTRSTSQTFIYKCDAVLCTLPLGVLKQQPPAVQFVPPLPEWKTSAVQRMGFGNLNKVVLCFDRVFWDPSVNLFGHVGSTTASRGELFLFWNLYKAPILLALVAGEAAGIMENISDDVIVGRCLAILKGIFGSSAVPQPKETVVSRWRADPWARGSYSYVAAGSSGNDYDLMAQPITPGPSIPGAPQPIPRLFFAGEHTIRNYPATVHGALLSGLREAGRIADQFLGAMYTLPRQATPGVPAQQSPSM | ||
| Apparent Molecular Weight | Approximately 93 kDa | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | LSD1 demethylates H3K4 to produce formaldehyde, and then in the presence of ammonia, formaldehyde reacts with acetyl linear aniline to produce fluorescent products. The specific activity is 36.565 ng/min/mg. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 2 mM DTT, pH 8.0, 10% Glycerol . Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. | ||
The LSD1 protein is a histone demethylase that modifies histone H3 by demethylating “Lys-4” (H3K4me) and “Lys-9” (H3K9me).It acts as a coactivator or corepressor, oxidizing substrates via FAD to generate imines that are subsequently hydrolyzed.LSD1 Protein, Human (sf9, His-GST) is the recombinant human-derived LSD1 protein, expressed by Sf9 insect cells , with N-His, N-GST labeled tag.
LSD1 demethylates H3K4 to produce formaldehyde, and then in the presence of ammonia, formaldehyde reacts with acetyl linear aniline to produce fluorescent products. The specific activity is 36.565 ng/min/mg.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.