Lentinan
(Synonyms: 香菇多糖) 目录号 : GC18405
Lentinan是一种从香菇（Lentinula edodes）中提取的天然β-葡聚糖，是一种非特异性免疫增强剂。
Cas No.:37339-90-5
Sample solution is provided at 25 µL, 10mM.
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Lentinan, a natural β-glucan extracted from the shiitake mushroom (Lentinula edodes), acts as a non-specific immune enhancer[1]. Lentinan enhances immune responses by activating macrophages, dendritic cells, and natural killer cells, and by promoting the expression of pro-inflammatory cytokines such as TNF-α and IL-6[2]. Lentinan is commonly used in research related to anti-tumor, anti-inflammatory, and neuroprotective effects[3].
In vitro, pretreatment of bovine mammary epithelial cells with Lentinan (100μg/mL; 6h) abolished LPS-induced ROS, restored SOD/T-AOC, up-regulated Nrf2/HO-1, silenced NF-κB/MAPK, suppressed IL-1β/IL-6/TNF-α and blocked apoptosis without cytotoxicity[4]. Treatment of A549 lung cancer cells with Lentinan (100μM; 48h) reduced IL-6 and IL-1β levels, decreased caspase-1 activity, raised ROS, lowered GPx4, depolarized mitochondria and activated caspase-3/DNA damage[5].
In vivo, Lentinan (10mg/kg/day; i.p.; 5 days) reversed house dust mite(HDM)-induced airway eosinophilia, IL-4/5/13 overproduction, mucus hypersecretion and ZO-1 loss in HDM-sensitized C57BL/6 mice[6]. Lentinan (200mg/kg/day; p.o.; 14 days) reversed antibiotic-induced colitis in C57BL/6J mice, restored colonic ZO-1/Occludin expression, reduced TNF-α and IL-6 levels, suppressed NF-κB activation, elevated propionic and butyric acid concentrations, reduced Proteobacteria abundance, and enriched SCFA-producing S24-7, Lactobacillus and Allobaculum[7].
References:
[1] Ahn H, Jeon E, Kim JC, et al. Lentinan from shiitake selectively attenuates AIM2 and non-canonical inflammasome activation while inducing pro-inflammatory cytokine production. Sci Rep. 2017;7(1):1314.
[2] Zhou G, Liu H, Yuan Y, Wang Q, Wang L, Wu J. Lentinan progress in inflammatory diseases and tumor diseases. Eur J Med Res. 2024;29(1):8.
[3] Liu S, Yan Z, Peng Y, et al. Lentinan has a beneficial effect on cognitive deficits induced by chronic Toxoplasma gondii infection in mice. Parasit Vectors. 2023;16(1):454.
[4] Meng M, Huo R, Wang Y, et al. Lentinan inhibits oxidative stress and alleviates LPS-induced inflammation and apoptosis of BMECs by activating the Nrf2 signaling pathway. Int J Biol Macromol. 2022;222(Pt B):2375-2391.
[5] Li M, Du X, Yuan Z, Cheng M, Dong P, Bai Y. Lentinan triggers oxidative stress-mediated anti-inflammatory responses in lung cancer cells. Mol Cell Biochem. 2022;477(2):469-477.
[6] Song Y, Chen Y, Cai H, et al. Lentinan attenuates allergic airway inflammation and epithelial barrier dysfunction in asthma via inhibition of the PI3K/AKT/NF-κB pathway. Phytomedicine. 2024;134:155965.
[7] Ji X, Su L, Zhang P, et al. Lentinan improves intestinal inflammation and gut dysbiosis in antibiotics-induced mice. Sci Rep. 2022;12(1):19609.
Lentinan是一种从香菇（Lentinula edodes）中提取的天然β-葡聚糖，是一种非特异性免疫增强剂[1]。Lentinan通过激活巨噬细胞、树突状细胞和自然杀伤细胞，促进TNF-α和IL-6等促炎因子的表达，从而增强免疫反应[2]。Lentinan常用于抗肿瘤、抗炎和神经保护等研究[3]。
在体外实验中，以100μg/mL Lentinan预处理牛乳腺上皮细胞6小时后，可消除LPS诱导的活性氧（ROS）生成，恢复SOD和总抗氧化能力（T-AOC），上调Nrf2/HO-1表达，抑制NF-κB/MAPK信号通路，抑制IL-1β/IL-6/TNF-α表达，并阻断细胞凋亡，且无细胞毒性[4]。以100μM Lentinan处理A549肺癌细胞48小时后，可降低IL-6和IL-1β水平，降低caspase-1活性，提高ROS水平，降低GPx4表达，使线粒体膜去极化，并激活caspase-3/DNA损伤[5]。
在体内实验中，Lentinan（10mg/kg/天；腹腔注射；5天）可逆转尘螨（HDM）诱导的C57BL/6小鼠气道嗜酸性粒细胞增多、IL-4/5/13过度表达、黏液分泌过多及ZO-1丢失[6]。Lentinan（200mg/kg/天；灌胃；14天）可逆转C57BL/6J小鼠的抗生素诱导性结肠炎，恢复结肠ZO-1/Occludin表达，降低TNF-α和IL-6水平，抑制NF-κB激活，提高丙酸和丁酸浓度，减少变形菌门丰度，并富集产短链脂肪酸的S24-7、乳酸菌和Allobaculum[7]。
| Cell experiment [1]: | |
Cell lines | A549 cells |
Preparation Method | The A549 cells were cultured in DMEM (Dulbecco's Modified Eagle's Medium) medium supplemented with 4mM L-glutamine and 10% fetal bovine serum (FBS), 100Units/ml penicillin and 100μg/ml streptomycin. The cells were incubated at 37°C, 5% CO2, and 95% air. Prior to each experiment 3.0×105 or 2.0×104 cells were seeded in a 6-well plate or 96-well plate, respectively. Cells were treated with 100µM Lentinan for 48h. Then cytotoxicity was assessed using the CCK-8 assay. Cellular Reactive Oxygen Species Detection Assay Kit was used according to manufacturer to quantify the ROS production. GPx4 activity were measured by the NADPH-coupled assay, using hydrogen peroxide and phosphatidylcholine hydroperoxide as the substrate. The caspase-1 activity was recorded using YVAD-AFC substrate at 505nm. |
Reaction Conditions | 100μM; 48h |
Applications | Treatment of A549 lung cancer cells with Lentinan reduced caspase-1 activity, raised ROS, and lowered GPx4 level. |
| Animal experiment [2]: | |
Animal models | Female C57BL/6 mice |
Preparation Method | Female C57BL/6 mice, aged between 6 to 8 weeks were anesthetized with isoflurane and intranasally administered 40μl of a 0.25mg/ml house dust mite(HDM) extract by Greer Laboratories on days 1-3 for sensitization. On days 8-12, they were intraperitoneally injected with Lentinan at dosages of 10mg/kg, 1 hour prior to HDM challenge. The control group received intranasal administration of PBS during sensitization and challenge. 48h after the last administration, mice were euthanized under anesthesia with avertin, and their organs were dissected for further Histopathological examination, qRT-PCR and Western blotting analysis. To collect the BALF, the trachea and lungs were flushed three times using 1ml of PBS. After centrifugation (300g, 10min, 4°C), the supernatant of BALF was harvested for further analysis. CellDrop® and flow cytometry were used for cell classifying and counting in BALF sediment. The presence of neutrophils was confirmed by their CD45+Ly6G+ immunophenotype, and eosinophils were identified through their CD45+ absence of Ly6G, CD11c, and presence of SiglecF. |
Dosage form | 10mg/kg/day; i.p.;5 days |
Applications | Lentinan reversed HDM-induced airway eosinophilia, IL-4/5/13 overproduction, mucus hypersecretion and ZO-1 loss in HDM-sensitized C57BL/6 mice. |
References: | |
| Cas No. | 37339-90-5 | SDF | |
| 别名 | 香菇多糖 | ||
| 分子式 | (C₄₂H₇₀O₃₅)nH₂O | 分子量 | 1134.98 |
| 溶解度 | 0.1 M NaOH : 33.33 mg/mL (ultrasonic and adjust pH to 9 with NaOH) | 储存条件 | Store at -20°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 881.1 μL | 4.4054 mL | 8.8107 mL |
| 5 mM | 176.2 μL | 881.1 μL | 1.7621 mL |
| 10 mM | 88.1 μL | 440.5 μL | 881.1 μL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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工作液浓度： mg/ml；
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2.
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