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Janelia Fluor 635, SE

(Synonyms: JF635, SE; JF635, NHS) 目录号 : GC50444

Red dye (Reactive group: NHS ester). Applications: super resolution microscopy, confocal, flow and live cell imaging

Janelia Fluor 635, SE Chemical Structure

规格 价格 库存 购买数量
2 mg
¥6,062.00
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Sample solution is provided at 25 µL, 10mM.

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产品描述

Red fluorogenic fluorescent dye; supplied as an NHS ester for coupling to primary amine groups. NHS ester can be converted to relevant substrate for use in self-labeling tag systems, e.g. HaloTag® and SNAP-tag®. Suitable for confocal fluorescent imaging and super resolution microscopy (SRM) techniques, such as dSTORM (live and fixed cells). Also suitable for flow cytometry. Cell permeable. Excitation maximum = 635 nm; emission maximum = 652 nm; Quantum yield = 0.56; Max extinction coefficient = 167,000 M-1cm-1 (measured in Ethanol or TFE plus 0.1% TFA); Correction factor = 0.0524. Exhibits low nonspecific background staining. To measure the absorbance spectrum of this dye we recommend the following solvent: ethanol or trifluoroethanol plus 0.1% TFA. We also offer Janelia Fluor® conjugated antibodies and custom conjugation services with our sister company Novus Biologicals. HaloTag is a trademark of Promega Corporation, and SNAP-tag is a trademark of New England BioLabs, Inc.

Grimm et al (2017) A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat.Methods 14 987 PMID:28869757

Chemical Properties

Cas No. SDF
别名 JF635, SE; JF635, NHS
Canonical SMILES O=C(ON1C(CCC1=O)=O)C2=CC=C(C([O-])=O)C(C(C3=CC=C(N4CC(F)C4)C=C3[Si]5(C)C)=C(C=C/6)C5=CC6=[N+]7CC(F)C\7)=C2
分子式 C33H29F2N3O6Si 分子量 629.69
溶解度 Soluble in DMSO 储存条件 Store at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

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1 mg 5 mg 10 mg
1 mM 1.5881 mL 7.9404 mL 15.8808 mL
5 mM 0.3176 mL 1.5881 mL 3.1762 mL
10 mM 0.1588 mL 0.794 mL 1.5881 mL
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