Isoshaftoside
(Synonyms: 异夏佛塔苷) 目录号 : GN10438
Isoshaftoside是一种C-糖苷黄酮类化合物，可从西番莲、紫花地丁和广东相思子等植物中分离出来，能够抑制发芽的独脚金根尖的生长。
Cas No.:52012-29-0
Sample solution is provided at 25 µL, 10mM.
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Isoshaftoside is a C-glycosylflavonoid and can be isolated from plants like Passiflora incarnata L., Viola yedoensis Makino, and Abrus cantoniensis, and it can inhibit the growth of germinating S. hermonthica radicles[1][2][3]. Isoshaftoside exert several pharmacological effects, such as anti inflammatory[4] and antioxidant effects[5].
Isoshaftoside (1µM; 12d) treatment can restore mitochondrial function and reduce glycolysis dependence in human dermal fibroblasts[6]. In HepG2 cells, treatment with Isoshaftoside at 300µM for 24 hours reduced the expression levels of LC3-II and p62[7].
Isoshaftoside (20mg/kg/day; 4 weeks; ip) treatment can reduce the body weight of high-fat diet mice and alleviate hepatic steatosis in nonalcoholic fatty liver disease(NAFLD)[7]. Isoshaftoside (50µM; 12 weeks; oral) treatment significantly decreased lobular inflammation fibrosis induced by a high-fat diet[8].
References:
[1]. Toda K, Hitoe S, Takeda S, et al. Passionflower Extract Induces High-amplitude Rhythms without Phase Shifts in the Expression of Several Circadian Clock Genes in Vitro and in Vivo. Int J Biomed Sci. 2017 Jun;13(2):84-92. PMID: 28824345; PMCID: PMC5542920.
[2]. Hu XL, Niu YJ, Chen M, et al. Preventive Effects of Total Flavonoid C-Glycosides from Abrus mollis on Nonalcoholic Fatty Liver Disease through Activating the PPARα Signaling Pathway. Planta Med. 2019 May;85(8):678-688. doi: 10.1055/a-0895-5838. Epub 2019 Apr 26. PMID: 31026873.
[3]. Hooper AM, Tsanuo MK, Chamberlain K, et al. Isoschaftoside, a C-glycosylflavonoid from Desmodium uncinatum root exudate, is an allelochemical against the development of Striga. Phytochemistry. 2010 Jun;71(8-9):904-8. doi: 10.1016/j.phytochem.2010.02.015. Epub 2010 Mar 6. PMID: 20211477.
[4]. Guan S, Sun L, Wang X, et al. Isoschaftoside Inhibits Lipopolysaccharide-Induced Inflammation in Microglia through Regulation of HIF-1α-Mediated Metabolic Reprogramming. Evid Based Complement Alternat Med. 2022 Nov 23;2022:5227335. doi: 10.1155/2022/5227335. PMID: 36467557; PMCID: PMC9711954.
[5]. Congyou DENG, Xinhe LEI, Minyou HE, et al. Identifying the active antioxidant ingredients in Arisaema Cum Bile aqueous extract on the basis of spectrum-effect relationships. Chinese Journal of Analytical Chemistry,Volume 52, Issue 12,2024,100453,ISSN 1872-2040.https://doi.org/10.1016/j.cjac.2024.100453.
[6]. Lee YH, So BH, Lee KS, et al. Identification of Cellular Isoschaftoside-Mediated Anti-Senescence Mechanism in RAC2 and LINC00294. Molecules. 2024 Sep 4;29(17):4182. doi: 10.3390/molecules29174182. PMID: 39275030; PMCID: PMC11397025.
[7]. Su Y, Kang Y, Yi J, et al. Isoschaftoside Reverses Nonalcoholic Fatty Liver Disease via Activating Autophagy In Vivo and In Vitro. Evid Based Complement Alternat Med. 2022 Jun 27;2022:2122563. doi: 10.1155/2022/2122563. PMID: 35795282; PMCID: PMC9252632.
[8]. Abe T. Isoschaftoside in Fig Leaf Tea Alleviates Nonalcoholic Fatty Liver Disease in Mice via the Regulation of Macrophage Polarity. Nutrients. 2025 Feb 21;17(5):757. doi: 10.3390/nu17050757. PMID: 40077628; PMCID: PMC11902273.
Isoshaftoside是一种C-糖苷黄酮类化合物，可从西番莲、紫花地丁和广东相思子等植物中分离出来，能够抑制发芽的独脚金根尖的生长[1][2][3]。Isoshaftoside具有多种药理作用，如抗炎[4]和抗氧化作用[5]。
Isoshaftoside（1μM；12天）处理可以恢复人皮肤成纤维细胞的线粒体功能并减少其对糖酵解的依赖[6]。在HepG2细胞中，用300μM Isoshaftoside处理24小时可降低LC3-II和p62的表达水平[7]。
Isoshaftoside（20mg/kg/天；4周；腹腔注射）处理可以减少高脂饮食小鼠的体重并减轻非酒精性脂肪性肝病（NAFLD）中的肝脂肪变性[7]。Isoshaftoside（50μM；12周；口服）处理显著减少高脂饮食引起的肝小叶炎症和纤维化[8]。
| Cell experiment [1]: | |
Cell lines | Human dermal fibroblasts |
Preparation Method | Senescent human dermal fibroblasts were treated with 4µM Isoshaftoside for 12 days. Then cells were stained with 10μg/mL DHR123 and ROS was measured by FACS. |
Reaction Conditions | 4µM; 12 days |
Applications | Isoshaftoside treatment significantly reduces reactive oxygen species (ROS) levels in senescent cells. |
| Animal experiment [2]: | |
Animal models | Nonalcoholic fatty liver disease (NAFLD) model mice |
Preparation Method | NAFLD animal model was conducted using C57 mice fed with a high-fat diet. NAFLD model mice were treated with Isoshaftoside (20mg/kg/day) for 4 weeks. Blood samples were collected and serum levels of alanine transaminase (ALT), aspartate transaminase (AST), fasting blood glucose (GLU), total cholesterol (TC), and triglyceride (TG) were examined with blood index kits. |
Dosage form | 20mg/kg/day; 4 weeks; ip. |
Applications | Isoshaftosidee reversed the damage to liver function and the elevation of blood glucose caused by a high-fat diet. |
References: | |
| Cas No. | 52012-29-0 | SDF | |
| 别名 | 异夏佛塔苷 | ||
| 化学名 | 5,7-dihydroxy-2-(4-hydroxyphenyl)-8-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-(hydroxymethyl)oxan-2-yl]-6-[(2S,3R,4S,5S)-3,4,5-trihydroxyoxan-2-yl]chromen-4-one | ||
| Canonical SMILES | C1C(C(C(C(O1)C2=C(C3=C(C(=C2O)C4C(C(C(C(O4)CO)O)O)O)OC(=CC3=O)C5=CC=C(C=C5)O)O)O)O)O | ||
| 分子式 | C26H28O14 | 分子量 | 564.49 |
| 溶解度 | ≥ 56.4mg/mL in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 1.7715 mL | 8.8576 mL | 17.7151 mL |
| 5 mM | 0.3543 mL | 1.7715 mL | 3.543 mL |
| 10 mM | 0.1772 mL | 0.8858 mL | 1.7715 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet