IPPD-Q
(Synonyms: N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone) 目录号 : GC91672IPPD-Q是N,N'-取代对苯二胺类化合物（PPD）的氧化产物，消耗二硫苏糖醇（DTT）的氧化潜势值为0.93μM min–1μM–1。
Cas No.:68054-73-9
Sample solution is provided at 25 µL, 10mM.
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IPPD-Q is the oxidation products of N,N'-Substituted p-phenylenediamines (PPD), with oxidative potential values of 0.93μM min–1μM–1for dithiothreitol (DTT) consumption rate[1-2]. IPPD-Q has been used in air pollution research and pollutant diagnosis research[3].
In vitro, IPPD-Q exhibits EC50 values of 2.97mg/L (30min) for Aquatic bacterium Vibrio fischeri[4]. IPPD-Q treatment (2.97mg/L) for 30min resulted in a significant decrease in metabolic activity and esterase activity and an increase in the degree of membranocyte damage and oxidative stress in Vibrio fischeri[4]. Treatment of hCMEC-D3 cells with 1.00μM IPPD-Q for 24 hours induced cell apoptosis, endothelial-mesenchymal transition, upregulated Plasminogen activator inhibitor-1 (PAI-1) expression, and triggered blood-brain barrier dysfunction[5].
References:
[1] Wang W, Cao G, Zhang J, et al. p-Phenylenediamine-derived quinones as new contributors to the oxidative potential of fine particulate matter[J]. Environmental Science & Technology Letters, 2022, 9(9): 712-717.
[2] Wang W, Cao G, Zhang J, et al. Beyond substituted p-Phenylenediamine antioxidants: Prevalence of their quinone derivatives in PM2. 5[J]. Environmental Science & Technology, 2022, 56(15): 10629-10637.
[3] Mao T, Liu W, Deng J, et al. p-Phenylenediamines and p-phenylenediamine quinone derivatives in rubber consumer products and typical urban dust: Sources, transformation profiles, and health risks[J]. Environment International, 2024, 192: 109042.
[4] Wang W, Chen Y, Fang J, et al. Toxicity of substituted p-phenylenediamine antioxidants and their derived novel quinones on aquatic bacterium: Acute effects and mechanistic insights[J]. Journal of Hazardous Materials, 2024, 469: 133900.
[5] Han M, Xia K, Xue Y, et al. Emergence of More Potent PPD-Qs beyond 6PPD-Q in Human Blood and Cerebrospinal Fluid[J]. Environmental Science & Technology Letters, 2025.
IPPD-Q是N,N'-取代对苯二胺类化合物（PPD）的氧化产物，消耗二硫苏糖醇（DTT）的氧化潜势值为0.93μM min–1μM–1[1-2]。IPPD-Q已被应用于大气污染研究和污染物溯源诊断研究[3]。
在体外，IPPD-Q对水生细菌费氏弧菌的EC50值（30分钟）为2.97mg/L[4]。IPPD-Q处理（2.97mg/L）30分钟会显著降低费氏弧菌的代谢活性和酯酶活性，同时增加细胞膜损伤程度和氧化应激水平[4]。在人脑血管内皮细胞（hCMEC-D3）中，1.00μM浓度的IPPD-Q处理24小时可诱导细胞凋亡和内皮-间质转化，上调纤溶酶原激活物抑制因子-1（PAI-1）的表达，并引发血脑屏障功能障碍[5]。
| Cell experiment [1]: | |
Cell lines | hCMEC-D3 cell |
Preparation Method | hCMEC-D3 cells were grown in endothelial cell medium (ECM) which included 5% fetal bovine serum (FBS) and 1% endothelial cell growth supplement along with 1% penicillin/streptomycin solution. Under 37 °C conditions with 5% CO2 humidified atmosphere the cell culture medium was changed every two days until cells reached 90% confluency. For 24 hours cells underwent treatment with different concentrations of IPPD-Q including 0, 0.01, 0.10, and 1.00μM. Collect cells from various exposure groups for protein expression analysis using Western blotting assays. Assess whether atorvastatin at a concentration of 0.30μM could modify the cellular effects of IPPD-Q when cells received both treatments simultaneously. The vehicle control that consisted of 0.1% DMSO (v/v) showed negligible effects after its evaluation process and thus became the designated exposure group 0. |
Reaction Conditions | 0, 0.01, 0.10, and 1.00μM; 24h |
Applications | IPPD-Q can increase the level of TNF-α, induce cell apoptosis, up-regulate PAI-1 and lead to endothelial-to-mesenchymal transition of hCMEC-D3 cells, resulting in endothelial cell dysfunction. |
References: | |
| Cas No. | 68054-73-9 | SDF | Download SDF |
| 别名 | N-Isopropyl-N'-phenyl-p-phenylenediamine-quinone | ||
| 分子式 | C15H16N2O2 | 分子量 | 256.3 |
| 溶解度 | Acetonitrile: Slightly soluble: 0.1-1 mg/ml,DMSO: Sparingly soluble: 1- 10 mg/ml | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.9017 mL | 19.5084 mL | 39.0168 mL |
| 5 mM | 0.7803 mL | 3.9017 mL | 7.8034 mL |
| 10 mM | 0.3902 mL | 1.9508 mL | 3.9017 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet