IFN b Mouse, HEK293, Fc
目录号 : GC27255IFN-β 蛋白是一种 I 型干扰素，通过与 IFNAR2 和 IFNAR1 受体结合并激活 Jak-STAT 信号传导，在先天免疫反应中发挥关键作用。这导致干扰素调节基因的转录调节，影响抗病毒蛋白、细胞增殖调节剂和免疫调节蛋白。IFN-beta 蛋白, Mouse (HEK293, Fc) 是重组的 IFN-β 蛋白，由 HEK293 表达，带有 C-hFc 标签
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
| Purity | Greater than 95% as determined by reducing SDS-PAGE. | Source | HEK293 |
| Phycical Appearance | Lyophilized powder | Shipping Condition | Room temperature in continental US; may vary elsewhere. |
| Apparent Molecular Weight | Approximately 55-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation. | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | 1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV) and the ED50 is typically 8 pg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IFNAR2 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse IFNB1. The ED50 for this effect is 3.885 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IFNAR2 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse IFNB1. The ED50 for this effect is 3.885 ng/mL. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5%mannitol, 0.01% tween-80 or PBS, pH 7.4. | ||
IFN-β 蛋白是一种 I 型干扰素细胞因子，在针对感染、肿瘤和各种炎症刺激的先天免疫反应中发挥着关键作用。其信号传导涉及与高亲和力受体 IFNAR2 和低亲和力受体 IFNAR1 结合，形成异二聚体复合物并激活经典的 Jak-STAT 信号传导途径。这种激活导致干扰素调节基因的转录调节，包括抗病毒蛋白、细胞增殖和分化的调节因子以及免疫调节蛋白。虽然主要通过 IFNAR1-IFNAR2 异二聚体受体发出信号，但 IFN-β 也可以单独与 IFNAR1 一起发挥作用，独立于 Jak-STAT 途径发挥作用。IFN-β 引发多种反应，包括抗病毒和抗菌活性，并影响 B 细胞发育、骨髓生成和脂多糖 (LPS) 诱导的肿瘤坏死因子的产生。除了其免疫功能外，IFN-β 通过调节多巴胺更新和保护多巴胺能神经元、促进神经元自噬、促进 α-突触核蛋白清除，从而防止多巴胺能神经元丢失，在神经元稳态中发挥着至关重要的作用。值得注意的是，IFN-β 在诱导细胞凋亡和抗增殖途径方面比干扰素 α (IFN-α) 表现出更强的效力，这对控制肿瘤细胞生长至关重要。它在这些监管过程中充当单体。
1. Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV) and the ED50 is typically 8 pg/mL. 2. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IFNAR2 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse IFNB1. The ED50 for this effect is 3.885 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant Mouse IFNAR2 at 2 μg/mL (100 μL/well) can bind Biotinylated Recombinant Mouse IFNB1. The ED50 for this effect is 3.885 ng/mL.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.