Hyaluronidase from Bovine Testes
(Synonyms: 玻璃酸酶) 目录号 : GC19788
Hyaluronidase from Bovine Testes是从牛睾丸中提取的内糖苷酶，属于透明质酸酶家族。
Cas No.:9001-54-1;37326-33-3
Sample solution is provided at 25 µL, 10mM.
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Hyaluronidase from Bovine Testes is an endoglycosidase extracted from bovine testicles and belongs to the hyaluronidase family [1]. Hyaluronidase cuts the β-1,4 glycosidic bond of hyaluronic acid, reduces the viscosity and barrier function of the extracellular matrix, and thus increases the diffusion capacity of other substances (such as drugs or body fluids) in tissues [2-3]. Hyaluronidase is often used to enhance the diffusion of drugs or molecules in tissues, and is also used for cell separation, local anesthesia assistance or tumor drug penetration research [4].
In L929 cells, Hyaluronidase (0-200U/mL; 12-24h) increased L929 cell growth by approximately 5-10% within 16 hours [5]. In periodontal ligament cells,the cell proliferation was inhibited by the treatment with Hyaluronidase (15–150U/mL; 10d) [6]. In articular chondrocytes, induction of CD44 and MMP expression by Hyaluronidase (0-200U/mL; 7d) treatment [7].
In melanoma models, weekly injection of high-dose Hyaluronidase (100000IU/kg; sc; 18 weeks) around the tumor and 0.3mg/kg vinblastine 4h after injection near the tumor significantly inhibited tumor growth [8].
References:
[1]. Vartanian AJ, Frankel AS, Rubin MG. Injected hyaluronidase reduces restylane-mediated cutaneous augmentation. Archives of Facial Plastic Surgery. 2005 Jul 1.
[2]. Cohen BE, Bashey S, Wysong A. The use of hyaluronidase in cosmetic dermatology: a review of the literature. J Clin Investigat Dermatol. 2015; 3(2): 7.
[3]. Ticona LA, Sánchez-Corral JS, Martín CD, et al. Rubus urticifolius Compounds with Antioxidant Activity, and Inhibition Potential against Tyrosinase, Melanin, Hyaluronidase, Elastase, and Collagenase. Pharmaceuticals. 2024 Jul 13; 17(7): 937.
[4]. Jung H. Hyaluronidase: An overview of its properties, applications, and side effects. Archives of plastic surgery. 2020 Jul;4 7(04): 297-300.
[5]. Chang NS. Hyaluronidase enhancement of TNF-mediated cell death is reversed by TGF-β1. American Journal of Physiology-Cell Physiology. 1997 Dec 1; 273(6): C1987-C1994.
[6]. Tanimoto K, Nakatani Y, Tanaka N, et al. Inhibition of the proliferation of human periodontal ligament fibroblasts by hyaluronidase. Archives of oral biology. 2008 Apr 1; 53(4): 330-336.
[7]. Ohno-Nakahara M, Honda K, Tanimoto K, et al. Induction of CD44 and MMP expression by hyaluronidase treatment of articular chondrocytes. Journal of biochemistry. 2004 May 1; 135(5): 567-575.
[8]. Spruß T, Bernhardt G, Schönenberger H, et al. Hyaluronidase significantly enhances the efficacy of regional vinblastine chemotherapy of malignant melanoma. Journal of cancer research and clinical oncology. 1995 Apr; 121: 193-202.
Hyaluronidase from Bovine Testes是从牛睾丸中提取的内糖苷酶，属于透明质酸酶家族 [1]。Hyaluronidase能切断透明质酸的β-1,4糖苷键，降低细胞外基质的粘度和屏障功能，从而增加其他物质（如药物或体液）在组织中的扩散能力 [2-3]。Hyaluronidase常用于增强药物或分子在组织中的扩散，也用于细胞分离、局部麻醉辅助或肿瘤药物渗透研究 [4]。
在L929细胞中，Hyaluronidase（0-200U/mL；12-24h）在16小时内使L929细胞生长约5-10% [5]。在牙周膜细胞中，用Hyaluronidase（15-150U/mL；10d）治疗可抑制细胞增殖 [6]。在关节软骨细胞中，用Hyaluronidase（0-200U/mL；7d）治疗可诱导CD44和MMP的表达 [7]。
在黑色素瘤模型中，每周在肿瘤周围注射高剂量Hyaluronidase（100000IU/kg；sc；18周），并在注射后4小时在肿瘤附近注射0.3mg/kg长春花碱，可显著抑制肿瘤生长 [8]。
| Cell experiment [1]: | |
Cell lines | L929 cells |
Preparation Method | 100μL aliquots of L929 cells (2.5 × 105cells/mL) were dispensed onto 96-well microtiter plates, cultured for 24h, treated with Hyaluronidase (0-200U/mL) for 12-24h. |
Reaction Conditions | 0-200U/mL; 12-24h |
Applications | Hyaluronidase increased L929 cell growth by approximately 5-10% within 16 hours. |
| Animal experiment [2]: | |
Animal models | Melanoma models |
Preparation Method | Solid tumor pieces were implanted subcutaneously on day 0 into the right flank of 7- to 9- week-old nude mice with a mean body weight of 20g. For each experiment we used only animals of the same sex. In the systemic chemotherapy, in which the chemosensitivity of the several melanoma models was ascertained, the treatment was started on day 1 by weekly i.p. administration of the chemotherapeutic. In the regional chemotherapy, in which vinblastine was combined with Hyaluronidase, the treatment was started when the solid tumors were visible, measuring at least 3mm in diameter. The local s.c. tumor therapy was carried out weekly by injecting the agents 5mm caudally from the neoplasm, carefully avoiding mechanical irritation of the tumor mass. Hyaluronidase was administered s.c. 4h before vinblastine. The number of animals per treatment group ranged from 8 to 13 (for details see the legends to the figures). Tumor growth kinetics was recorded by measuring tumor diameters with an electronic caliper. Tumor areas were calculated as the product of two perpendicular diameters, one measured across the greatest width of the tumor. |
Dosage form | 100000IU/kg; sc; 18 weeks |
Applications | Weekly injection of high-dose Hyaluronidase around the tumor and 0.3mg/kg vinblastine 4h after injection near the tumor significantly inhibited tumor growth. |
References: | |
| Cas No. | 9001-54-1;37326-33-3 | SDF | |
| 别名 | 玻璃酸酶 | ||
| 分子式 | 分子量 | ||
| 溶解度 | 储存条件 | 4°C, protect from light | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
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