Guanidinosuccinic acid
(Synonyms: 胍基琥珀酸) 目录号 : GC31413A guanidino compound with epileptogenic activity
Cas No.:6133-30-8
Sample solution is provided at 25 µL, 10mM.
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- Purity: >97.00%
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Animal experiment: |
Mice[2]Randomly bred Swiss mice (male and female, body weight 13-25 g for the first series of experiments) are housed under standard environmentally controlled conditions. For another series of experiments, young Swiss mice 7, 14 and 21 days old are used. Injections of Guanidinosuccinic acid suspensions are delivered in volumes of 0.1 mL per 10 g body weight, i.p. and in doses between 250 and 1000 mg/kg (5 mice per dose, in duplicate). After injection, the mice are put in individual cylindrical transparent cages and observed for 1 h (for 2 h in the case of the young mice). Both CD50 and LD50 are calculated by probit analysis or moving average interpolation[2]. |
References: [1]. Milstien S, et al. Role of intestinal microflora in the metabolism of guanidinosuccinic acid. J Bacteriol. 1973 May;114(2):641-4. |
N-Amidino-L-aspartic acid is a guanidino compound found in mammalian sera and the CNS.1 It induces whole-cell currents in rat hippocampal slices, an effect that can be reversed by the NMDA receptor antagonists D-AP5 and ketamine. Intrahippocampal administration of N-amidino-L-aspartic acid induces status epilepticus and neuronal cell death in rats.2 Cerebrospinal fluid and serum levels of N-amidino-L-aspartic acid are increased in patients with renal failure.1,2
1.D'Hooge, R., Raes, A., Lebrun, P., et al.N-methyl-D-aspartate receptor activation by guanidinosuccinate but not by methylguanidine: Behavioural and electrophysiological evidenceNeuropharmacology35(4)433-440(1996) 2.Pan, J.C., Pei, Y.Q., An, L., et al.Epileptiform activity and hippocampal damage produced by intrahippocampal injection of guanidinosuccinic acid in ratNeurosci. Lett.209(2)121-124(1996)
Cas No. | 6133-30-8 | SDF | |
别名 | 胍基琥珀酸 | ||
Canonical SMILES | O=C(O)C[C@@H](C(O)=O)NC(N)=N | ||
分子式 | C5H9N3O4 | 分子量 | 175.14 |
溶解度 | DMSO : 75 mg/mL (428.23 mM);Water : 6 mg/mL (34.26 mM) | 储存条件 | Store at -20°C |
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1 mg | 5 mg | 10 mg | |
1 mM | 5.7097 mL | 28.5486 mL | 57.0972 mL |
5 mM | 1.1419 mL | 5.7097 mL | 11.4194 mL |
10 mM | 0.571 mL | 2.8549 mL | 5.7097 mL |
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Guanidinosuccinic acid in uremia
The pathobiochemistry of uremia and hyperargininemia further demonstrates a metabolic relationship between urea and guanidinosuccinic acid
To better understand the biosynthesis of guanidinosuccinic acid, we determined urea, arginine, and guanidinosuccinic acid levels in nondialyzed uremic and hyperargininemic patients. These substances were also determined during several years of therapy in one hyperarginiemic patient. Interrelationships of guanidinosuccinic acid levels with their corresponding urea and arginine levels were assessed by linear correlation studies. In uremic patients, a significant positive linear correlation (r = .821, p less than .001) was found between serum urea and guanidinosuccinic acid levels A significant positive linear correlation was also found between serum urea levels and urinary guanidinosuccinic acid levels (r = .828, P less than .001), but not between serum arginine levels and urinary guanidinosuccinic acid levels in hyperargininemic patients. In the intrahyperargininemic patient study, a similar significant positive correlation was found between serum urea levels and the corresponding urinary guanidinosuccinic acid levels (r = .866, P less than .001); the correlation between serum arginine levels and the corresponding urinary guanidinosuccinic acid levels was smaller. The presented analytical findings in uremic and hyperargininemic patients clearly demonstrate a metabolic relationship between urea and guanidinosuccinic acid.
The biosynthesis of guanidinosuccinic acid by perfused rat liver
The metabolic pathway for the synthesis of guanidinosuccinic acid was studied in the rat. Labeled guanidinosuccinic acid was isolated from the urine of animals given L-[guanidino-14C]arginine intraperitoneally but did not appear in the urine after administration of D,L-[guanidino-14C]canavanine. Radioactive arginine and nonradioactive aspartic acid and arginine were infused in the isolated, perfused rat liver. After 20 min, small amounts of both labeled and unlabeled guanidinosuccinic acid and large amounts of urea were detected in radiochromatograms of the perfusate. These results support the theory that guanidinosuccinic acid is formed in the liver from transamidination of arginine to aspartic acid.
Role of intestinal microflora in the metabolism of guanidinosuccinic acid
Among a variety of bacteria isolated from the gastrointestinal tracts of rats and humans, only streptococci of group N are capable of degrading guanidinosuccinic acid added to their culture medium. The urinary excretion of guanidinosuccinic acid by germfree rats is greater than that of conventional rats. The excretion of this compound by gnotobiotic rats correlates with the capacity of their intestinal microflora to degrade guanidinosuccinic acid in culture. Thus, guanidinosuccinic acid excretion is low in rats infected exclusively with Streptococcus faecalis, and the excretion is not altered when germfree rats are infected with an organism unable to degrade guanidinosuccinic acid (Lactobacillus). These findings suggest that the intestinal microflora, particularly Streptococcus, play a role in the metabolism of guanidinosuccinic acid by the host.