GDP-α-D-mannose disodium

GDP-α-D-mannose disodium serves as a donor substrate for mannosyltransferases and acts as the precursor of GDP-β-L-fucose. GDP-α-D-mannose disodium exhibits competitive inhibition against GTP with a Ki value of 14.7μM and uncompetitive inhibition against mannose-1-P with a Ki value of 115μM. This compound is a key intermediate in the biosynthesis of N-linked glycans, which are essential for proper protein folding, stability, and function in eukaryotic cells^[1]. Synthesis of GDP-α-D-mannose disodium occurs in the cytoplasm through a series of enzymatic reactions involving GDP-mannose pyrophosphorylase^[2]. The role of GDP-α-D-mannose disodium in forming complex glycan structures is crucial for cell-cell interactions, immune recognition, and signal transduction^[3].

References:
[1] Spiro RG. Protein glycosylation: nature, distribution, enzymatic formation, and disease implications of glycopeptide bonds. Glycobiology. 2002 Mar;12(4):43R-56R.
[2] Moremen KW, Tiemeyer M, Nairn AV. Vertebrate protein glycosylation: diversity, synthesis and function. Nat Rev Mol Cell Biol. 2012 Aug 24;13(7):448-62.
[3] Helenius A, Aebi M. Roles of N-linked glycans in the endoplasmic reticulum. Annu Rev Biochem. 2001;70:1-22.

GDP-α-D-mannose disodium(GDP-α-D-甘露糖二钠）是甘露糖转移酶的供体底物，并且是GDP-β-L-岩藻糖的前体。GDP-α-D-mannose disodium对GTP表现出竞争性抑制作用，Ki值为14.7μM；对甘露糖-1-磷酸表现出非竞争性抑制作用，Ki值为115μM。该化合物是N-连接糖链生物合成中的关键中间体，这种糖链对于真核细胞中蛋白质的正确折叠、稳定性和功能至关重要^[1]。GDP-α-D-mannose disodium的合成发生在细胞质中，通过一系列涉及GDP-甘露糖焦磷酸化酶的酶促反应完成^[2]。GDP-α-D-mannose disodium在形成复杂的糖链结构中发挥关键作用，这些结构对于细胞间相互作用、免疫识别和信号转导至关重要^[3]。