β-Glucan
(Synonyms: β-葡聚糖) 目录号 : GD13990β-Glucan是由D-葡萄糖单体通过β-糖苷键连接而成的多糖。β-Glucan具有降胆固醇、降血糖、免疫调节、抗肿瘤、抗氧化和抗炎等功能。
Cas No.:9012-72-0
Sample solution is provided at 25 µL, 10mM.
β-Glucan is a polysaccharide composed of D-glucose monomers linked by β-glycosidic bonds. β-Glucan has the functions of hypocholesterolemic, hypoglycemic, immunomodulatory, antitumor, antioxidant, and anti-inflammatory[1].
In vitro, β-Glucan (1, 2.5, 5, 10 and 20μg/mL; 24h) inhibits SVCV infection in ZF4 cells[2]. β-Glucan (1, 10, 100 and 200µg/ml; 48h) had a positive effect on increasing tumoricidal activity of macrophages[3].
In vivo, β-Glucan (0.025g/100g dry diet; 0-14 days) enhanced resistance of zebrafish against spring viraemia of carp virus (SVCV)[2]. In a mouse orthotopic pancreatic cancer model, β-Glucan (1mg/mice; once; i.p.) in combination with Irreversible electroporation (IRE) reduced local and distant tumor burden and prolonged survival[4].
References:
[1] Du B, Meenu M, Liu H, Xu B. A Concise Review on the Molecular Structure and Function Relationship of β-Glucan. Int J Mol Sci. 2019 Aug 18;20(16):4032.
[2] Liang H, Li Y, Li M, Zhou W, Chen J, Zhang Z, Yang Y, Ran C, Zhou Z. The effect and underlying mechanism of yeast β-glucan on antiviral resistance of zebrafish against spring viremia of carp virus infection. Front Immunol. 2022 Nov 3;13:1031962.
[3] Roudbary M, Daneshmand S, Hajimorad M, Roudbarmohammadip S, Hassan ZM. Immunomodulatory Effect of β-Glucan on Peritoneal Macrophages of Bab1/c Mice. Pol J Microbiol. 2015;64(2):175-9.
[4] Woeste MR, Shrestha R, Geller AE, Li S, Montoya-Durango D, Ding C, Hu X, Li H, Puckett A, Mitchell RA, Hayat T, Tan M, Li Y, McMasters KM, Martin RCG, Yan J. Irreversible electroporation augments β-glucan induced trained innate immunity for the treatment of pancreatic ductal adenocarcinoma. J Immunother Cancer. 2023 Apr;11(4):e006221.
β-Glucan是由D-葡萄糖单体通过β-糖苷键连接而成的多糖。β-Glucan具有降胆固醇、降血糖、免疫调节、抗肿瘤、抗氧化和抗炎等功能[1]。
体外实验中，β-Glucan(1, 2.5, 5, 10和20μg/mL; 24h)能够抑制SVCV在ZF4细胞中的感染[2]。此外，β-Glucan(1, 10, 100和200µg/ml; 48h)对增强巨噬细胞的杀肿瘤活性有积极作用[3]。
体内实验方面，β-Glucan(0.025g/100g干饲料; 0-14天)增强了斑马鱼对鲤春病毒血症病毒（SVCV）的抵抗力[2]。在小鼠原位胰腺癌模型中，β-Glucan(1mg/只小鼠; 一次; 腹腔注射)与不可逆电穿孔（IRE）联合使用可减轻局部和远处的肿瘤负荷，并延长生存时间[4]。
| Cell experiment [1]: | |
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Cell lines |
ZF4 cells |
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Preparation Method |
ZF4 cells were treated with β-Glucan (1, 2.5, 5, 10, 20μg/mL) for 24h. Then 0.1 MOI SVCV was added and incubated at 22°C for 1h. Infected ZF4 cells monolayers were then washed, fresh medium added, and plates further incubated. At 24 hours after infection, SVCV replication in ZF4 cells was evaluated by qPCR measuring the expression of SVCV N protein. ZF4 cells infected with SVCV but not treated with β-Glucan is a polysaccharide composed of D-glucose monomers linked by β-glycosidic bonds. were used as control. |
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Reaction Conditions |
1, 2.5, 5, 10, 20μg/mL; 24h |
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Applications |
β-Glucan (1, 2.5, 5, 10, 20μg/mL; 24h) inhibits SVCV infection in ZF4 cells. |
| Animal experiment [2]: | |
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Animal models |
Wild-type (WT) C57Bl/6 female and male mice, 6-8 weeks old |
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Preparation Method |
On day 0, 0.1×10⁶ KPC cells were orthotopically implanted into the pancreas of the mice. On day 7 after tumor implantation, the mice were treated with a single intraperitoneal injection of β-Glucan (1mg/mouse) or PBS control. On day 14, sham surgery or IRE ablation was performed. On day 24 after tumor implantation, representative images of orthotopic pancreatic KPC tumors were obtained from the control and different treatment groups. |
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Dosage form |
1mg/mice; once; i.p. |
|
Applications |
In a mouse orthotopic pancreatic cancer model, β-Glucan (1 mg/mice; once; i.p.) in combination with Irreversible electroporation (IRE) reduced local and distant tumor burden and prolonged survival. |
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References: |
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| Cas No. | 9012-72-0 | SDF | |
| 别名 | β-葡聚糖 | ||
| 分子式 | 分子量 | ||
| 溶解度 | DMSO : 100 mg/mL (Need ultrasonic); Water：100mg/mL | 储存条件 | Powder,-20°C,3 years;,4°C,2 years; |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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