DL-Ethionine
(Synonyms: DL-乙硫氨酸) 目录号 : GD11710DL-Ethionine是一种甲硫氨酸拮抗剂，具有毒性和致癌性。
Cas No.:67-21-0
Sample solution is provided at 25 µL, 10mM.
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DL-Ethionine, an antagonist of Methionine, is toxic and a carcinogen [1]. DL-Ethionine could increase the cyclic AMP content and stimulate the malignant transformation of cells [2]. DL-Ethionine has been widely used to induce hepatocarcinogenesis in rats[3].
In vivo, Feeding a low-choline diet containing 0.5% DL-ethionine resulted in the development of acute hemorrhagic pancreatitis with peritoneal fat necrosis in female mice within five days[4]. DL-Ethionine treatment via intraperitoneal injections for 10 days of DL-Ethionine (0.7mg/g) caused degenerative changes in rat pancreatic acinar tissue, including a widening of the endoplasmic reticulum, a reduction in the number of membrane-bound ribosomes, and the appearance of fine and bulky vacuoles containing nongranular, disordered membranes[5]. C3H female mice given 150mg/kg DL-Ethionine by intubation three times a week for 30 weeks resulted in a significant increase in the incidence of liver tumors[6].
References:
[1] Wang J, Yao H, Lu T, et al. Spectroscopic, kinetic, and theoretical analyses of oxidation of dl-ethionine by Pt (IV) anticancer model compounds[J]. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, 2019, 223: 117328.
[2] DeRubertis F R, Craven P A. Sequential alterations in the hepatic content and metabolism of cyclic AMP and cyclic GMP induced by DL-ethionine: Evidence for malignant transformation of liver with a sustained increase in cyclic AMP[J]. Metabolism, 1976, 25(12): 1611-1625.
[3] Kamamoto Y, Makiura S, Sugihara S, et al. The inhibitory effect of copper on DL-ethionine carcinogenesis in rats[J]. Cancer research, 1973, 33(5): 1129-1135.
[4] Lombardi B, Estes L W, Longnecker D S. Acute hemorrhagic pancreatitis (massive necrosis) with fat necrosis induced in mice by DL-ethionine fed with a choline-deficient diet[J]. The American journal of pathology, 1975, 79(3): 465.
[5] Herman L, Fitzgerald P J. The degenerative changes in pancreatic acinar cells caused by DL-ethionine[J]. The Journal of Cell Biology, 1962, 12(2): 277-296.
[6] Hoover K L, Hyde C L, Wenk M L, et al. Ethionine carcinogenesis in CD-1, BALB/c and C3H mice[J]. Carcinogenesis, 1986, 7(7): 1143-1148.
DL-Ethionine是一种甲硫氨酸拮抗剂，具有毒性和致癌性[1]。DL-Ethionine可通过提高细胞内环磷酸腺苷含量，刺激细胞发生恶性转化[2]。DL-Ethionine已广泛应用于大鼠肝癌模型的诱导[3]。
在体内，饲喂含0.5%的DL-Ethionine的低胆碱饲料可在五天内诱导雌性小鼠发生急性出血性胰腺炎并伴腹膜脂肪坏死[4]。每日腹腔注射0.7mg/g剂量的DL-Ethionine连续10天，可引起大鼠胰腺腺泡组织退行性病变，包括内质网扩张、膜结合核糖体数量减少，以及出现含非颗粒性无序膜的细小和粗大空泡[5]。每周三次通过插管给予150mg/kg剂量的DL-Ethionine连续30周，可显著提高C3H雌性小鼠的肝脏肿瘤发生率[6]。
| Animal experiment [1]: | |
Animal models | Male albino rats |
Preparation Method | One hundred and fifty male albino rats, Wistar strain, weighing 160 to 180g, were given water ad libitum and kept in individual cages in an air-conditioned room at 68°F. One hundred and twenty-five of the rats were placed on a protein-free diet. Seventy-five of the rats on the protein-free diet received daily intraperitoneal injections for 10 days of DL-Ethionine (0.7mg/g) dissolved in aqueous 0.9 sodium chloride (ethionine group). Fifty mice were kept on the protein-free diet. After day 10, the mice of both the DL-Ethionine and protein-free groups were maintained on the protein-free diet through day 28. The status of pancreatic acinar tissues in rats injected with ethionine was analyzed. |
Dosage form | 0.7mg/g/day for 10 days; i.p. |
Applications | DL-Ethionine treatment caused degenerative changes in rat pancreatic acinar tissue, including a widening of the endoplasmic reticulum, a reduction in the number of membrane-bound ribosomes, and the appearance of fine and bulky vacuoles containing nongranular, disordered membranes. |
References: | |
| Cas No. | 67-21-0 | SDF | |
| 别名 | DL-乙硫氨酸 | ||
| 分子式 | C6H13NO2S | 分子量 | 163.238 |
| 溶解度 | 储存条件 | -20°C, protect from light, away from moisture | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.126 mL | 30.6301 mL | 61.2602 mL |
| 5 mM | 1.2252 mL | 6.126 mL | 12.252 mL |
| 10 mM | 612.6 μL | 3.063 mL | 6.126 mL |
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| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
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| % DMSO % % Tween 80 % saline | ||||||||||
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计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
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2.
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Quality Control & SDS
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- Purity: >98.00%
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