Nonaethylene glycol monododecyl ether
(Synonyms: 聚醚醇,Nonaoxyethylene monododecyl ether) 目录号 : GC38697
Nonaethylene glycol monododecyl ether (Nonaoxyethylene monododecyl ether) 是一种非离子表面活性剂和聚乙二醇清洁剂，可用于形成初始聚结的 O/W 乳剂液滴，以及用于蛋白质分离和纯化。
Cas No.:3055-99-0
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Nonaethylene glycol monododecyl ether (Nonaoxyethylene monododecyl ether) is a nonionic surfactant and polyethylene glycol (PEG) detergent that can be used to form initial coalesced O/W emulsion droplets, as well as for protein separation and purification[1][2][3].
Examination of a series of non-ionic PEG detergents with several long-chain E-PDSs from different organisms reveals that in vitro incubations with Nonaethylene glycol monododecyl ether typically gave chain lengths that corresponded to those of the isoprenoid moieties in respiratory quinones synthesized in vivo[2].
[1]. Li C, et al. Oil-in-Water Emulsion Templated and Crystallization-Driven Self-Assembly Formation of Poly(l-lactide)-Polyoxyethylene-Poly(l-lactide) Fibers. Langmuir. 2017 Nov 14;33(45):13060-13067. [2]. Pan JJ, et al. Dependence of the product chain-length on detergents for long-chain E-polyprenyl diphosphate synthases. Biochemistry. 2013 Jul 23;52(29):5002-8. [3]. Zhang W, et al. Comparison of the different types of surfactants for the effect on activity and structure of soybean peroxidase. Langmuir. 2009 Feb 17;25(4):2363-8.
| Cas No. | 3055-99-0 | SDF | |
| 别名 | 聚醚醇,Nonaoxyethylene monododecyl ether | ||
| Canonical SMILES | CCCCCCCCCCCCOCCOCCOCCOCCOCCOCCOCCOCCOCCO | ||
| 分子式 | C30H62O10 | 分子量 | 582.81 |
| 溶解度 | DMSO: 250 mg/mL (428.96 mM); Ethanol: 100 mg/mL (171.58 mM) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.7158 mL | 8.5791 mL | 17.1583 mL |
| 5 mM | 0.3432 mL | 1.7158 mL | 3.4317 mL |
| 10 mM | 0.1716 mL | 0.8579 mL | 1.7158 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Cytomembrane-Inspired MXene Ink with Amphiphilic Surfactant for 3D Printed Microsupercapacitors
ACS Nano 2022 Sep 27;16(9):14723-14736.PMID:36001805DOI:10.1021/acsnano.2c05445.
Two-dimensional (2D) material-based hydrogels have been widely utilized as the ink for extrusion-based 3D printing in various electronics. However, the viscosity of the hydrogel ink is not high enough to maintain the self-supported structure without architectural deformation. It is also difficult to tune the microstructure of the printed devices using a low-viscosity hydrogel ink. Herein, by mimicking a phospholipid bilayer in a cytomembrane, the amphiphilic surfactant Nonaethylene glycol monododecyl ether (C12E9) was incorporated into MXene hydrogel. The incorporation of C12E9 offers amphiphilicity to the MXene flakes and produces a 3D interlinked network of the MXene flakes. The 3D interlinked network offers a high-viscosity, homogenized flake distribution and enhanced printability to the ink. This ink facilitates the alignment of the MXene flakes during extrusion as well as the formation of the aligned micro- and sub-microsized porous structures, leading to the improved electrochemical performance of the printed microsupercapacitor. This study provides an example for the preparation of microelectronics with tunable microstructures.
Determination of critical micelle concentrations and aggregation numbers by fluorescence correlation spectroscopy: aggregation of a lipopolysaccharide
Anal Chim Acta 2006 Jan 18;556(1):216-25.PMID:17723352DOI:10.1016/j.aca.2005.09.008.
Fluorescence correlation spectroscopy (FCS) is often used to determine the mass or radius of a particle by using the dependence of the diffusion coefficient on the mass and shape. In this article we discuss how the particle size of aggregates can be measured by using the concentration dependence of the amplitude of the autocorrelation function (ACF) instead of the temporal decay. We titrate a solution of aggregates or micelles with a fluorescent label that possesses a high affinity for these structures and measure the changes in the amplitude of the ACF. We develop the theory describing the change of the ACF amplitude with increasing concentrations of labels and use it to fit experimental data. It is shown how this method can determine the aggregation number and critical micelle concentration of a standard detergent Nonaethylene glycol monododecyl ether (C12E9) and a lipopolysaccharide (LPS: Escherichia coli 0111:B4).
Dependence of the product chain-length on detergents for long-chain E-polyprenyl diphosphate synthases
Biochemistry 2013 Jul 23;52(29):5002-8.PMID:23802587DOI:10.1021/bi400681d.
Long-chain E-polyprenyl diphosphate synthases (E-PDS) catalyze repetitive addition of isopentenyl diphosphate (IPP) to the growing prenyl chain of an allylic diphosphate. The polyprenyl diphosphate products are required for the biosynthesis of ubiquinones and menaquinones required for electron transport during oxidative phosphorylation to generate ATP. In vitro, the long-chain PDSs require addition of phospholipids or detergents to the assay buffer to enhance product release and maintain efficient turnover. During preliminary assays of product chain-length with anionic, zwitterionic, and nonionic detergents, we discovered considerable variability. Examination of a series of nonionic PEG detergents with several long-chain E-PDSs from different organisms revealed that in vitro incubations with Nonaethylene glycol monododecyl ether or Triton X-100 typically gave chain-lengths that corresponded to those of the isoprenoid moieties in respiratory quinones synthesized in vivo. In contrast, incubations in buffer with n-butanol, CHAPS, DMSO, n-octyl-β-glucopyranoside, or β-cyclodextrin or in buffer without detergent typically proceeded more slowly and gave a broad range of chain-lengths.
Intestinal surfactant permeation enhancers and their interaction with enterocyte cell membranes in a mucosal explant system
Tissue Barriers 2017 Jul 3;5(3):e1361900.PMID:28837408DOI:10.1080/21688370.2017.1361900.
Intestinal permeation enhancers (PEs) are agents aimed to improve oral delivery of therapeutic drugs with poor bioavailability. The main permeability barrier for oral delivery is the intestinal epithelium, and PEs act to increase the paracellular and/or transcellular passage of drugs. Transcellular passage can be achieved by cell membrane permeabilization and/or by endocytic uptake and subsequent transcytosis. One broad class of PEs is surfactants which act by inserting into the cell membrane, thereby perturbing its integrity, but little is known about how the dynamics of the membrane are affected. In the present work, the interaction of the surfactants lauroyl-L-carnitine, 1-decanoyl-rac-glycerol, and Nonaethylene glycol monododecyl ether with the intestinal epithelium was studied in organ cultured pig jejunal mucosal explants. As expected, at 2 mM, these agents rapidly permeabilized the enterocytes for the fluorescent polar tracer lucifer yellow, but surprisingly, they all also blocked both constitutive -and receptor-mediated pathways of endocytosis from the brush border, indicating a complete arrest of apical membrane trafficking. At the ultrastructural level, the PEs caused longitudinal fusion of brush border microvilli. Such a membrane fusogenic activity could also explain the observed formation of vesicle-like structures and large vacuoles along the lateral cell membranes of the enterocytes induced by the PEs. We conclude that the surfactant action of the PEs selected in this study not only permeabilized the enterocytes, but profoundly changed the dynamic properties of their constituent cell membranes.
Comparison of the different types of surfactants for the effect on activity and structure of soybean peroxidase
Langmuir 2009 Feb 17;25(4):2363-8.PMID:19161266DOI:10.1021/la803240x.
In the pH 2.6 and 5.2 systems, soybean peroxidase (SBP) (isoelectric point, pI 3.9) has positive and negative charge, respectively. In order to acquire detailed knowledge on the role played by electrostatics in the denaturation of proteins, a comparison of anionic surfactant sodium dodecyl sulfate (SDS), nonionic surfactant Nonaethylene glycol monododecyl ether [C12H25O(CH2CH2O)9H] (AEO9), and cationic surfactant cetyltrimethylammonium bromide (CTAB) for the influences on the activity and structure of soybean peroxidase (SBP) was carried out by measuring the activity, far-UV circular dichrosm, fluorescence, and electronic absorption spectra of SBP in the pH 2.6 and 5.2 systems at 30 degrees C. In the pH 2.6 systems, the interaction of SDS with SBP results in an increase in the fluorescence intensity with a red shift of the emission maximum of the tryptophan fluorescence and a blue shift of the Soret band. In the meantime, the alpha-helix of SBP is unfolded and the activity of SBP is lost irreversibly. In pH 5.2 systems, the fluorescence spectra features of SBP are similar to those in pH 2.6 systems with increasing SDS concentration, but a red shift of Soret band as well as an alteration of the tertiary structure of SBP occurs, and the lost activity is recoverable. The electrostatic interactions between SBP and SDS play an important role in the denaturation of SBP. The effects of AEO9 and CTAB in pH 2.6 and 5.2 systems on the activity and spectral features of SBP are similar to that of SDS in pH 5.2 systems, but AEO9 is prone to unfold the beta-sheet of SBP in pH 2.6 systems. The electrostatic interactions of CTAB with SBP are not the primary elements for denaturation of SBP, which distinctly differ from those of SDS. These results can be useful with respect to wide applications of the surfactants in the separation and purification of proteins.