Dehydroevodiamine hydrochloride
(Synonyms: 盐酸去氢吴茱萸碱) 目录号 : GC38435
An alkaloid with diverse biological activities
Cas No.:111664-82-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Dehydroevodiamine is an alkaloid that has been isolated from E. rutaecarpa and has anti-inflammatory, antiarrhythmic, hypotensive, and anti-amnesic biological activities.1,2,3,4 It inhibits LPS-induced secretion of prostaglandin E2 in RAW 264.7 mouse macrophages when used at a concentration of 30 μM.1 Dehydroevodiamine (0.1-0.3 μM) reduces the upstroke velocity, action potential amplitude, and contractile force of electrically-stimulated primary human atrial trabeculae.2 In vivo, dehydroevodiamine (10 mg/kg) decreases mean arterial blood pressure (MAP) by approximately 30% in rats.3 Dehydroevodiamine (1.5 mg/kg) also inhibits amnesia induced by amyloid-β (25-35) and increases latency to step-through in a passive avoidance test in mice.4
1.Noh, E.J., Ahn, K.S., Shin, E.M., et al.Inhibition of lipopolysaccharide-induced iNOS and COX-2 expression by dehydroevodiamine through suppression of NF-κB activation in RAW 264.7 macrophagesLife Sci.79(7)695-701(2006) 2.Loh, S.-H., Tsai, Y.-T., Lee, C.-Y., et al.Antiarrhythmic effects of dehydroevodiamine in isolated human myocardium and cardiomyocytesJ. Ethnopharmacol.153(3)753-762(2014) 3.Yang, M.C.M., Wu, S.-L., Kuo, J.-S., et al.The hypotensive and negative chronotropic effects of dehydroevodiamineEur. J. Pharmacol.182(3)537-542(1990) 4.Wang, H.-H., Chou, C.-J., Liao, J.-F., et al.Dehydroevodiamine attenuates β-amyloid peptide-induced amnesia in miceEur. J. Pharmacol.413(2-3)221-225(2001)
| Cas No. | 111664-82-5 | SDF | |
| 别名 | 盐酸去氢吴茱萸碱 | ||
| Canonical SMILES | O=C1N2C(N(C)C3=C1C=CC=C3)=C4C(CC2)=C5C=CC=CC5=N4.[H]Cl | ||
| 分子式 | C19H16ClN3O | 分子量 | 337.8 |
| 溶解度 | DMF: 2.5 mg/ml,DMSO: 12.5 mg/ml,Ethanol: 2.5 mg/ml | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9603 mL | 14.8017 mL | 29.6033 mL |
| 5 mM | 0.5921 mL | 2.9603 mL | 5.9207 mL |
| 10 mM | 0.296 mL | 1.4802 mL | 2.9603 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Increased feline cerebral blood flow induced by Dehydroevodiamine hydrochloride from Evodia rutaecarpa
J Nat Prod 1994 Mar;57(3):387-9.PMID:8201313DOI:10.1021/np50105a009.
Dehydroevodiamine hydrochloride (0.1-0.3 mg/kg iv), which was isolated from the leaves of Evodia rutaecarpa, increased the cerebral blood flow recorded from the surface of the supra-sylvian gyrus in anesthetized cats. This action reached a maximum 1-4 min after injection and continued for 10 min. However, the compound had negligible effects on other cardiorespiratory functions at the doses examined. These results suggest that the compound selectively increases cerebral blood flow.
Novel anticholinesterase and antiamnesic activities of dehydroevodiamine, a constituent of Evodia rutaecarpa
Planta Med 1996 Oct;62(5):405-9.PMID:8923803DOI:10.1055/s-2006-957926.
To find a new compound with antiamnesic activity, we screened 29 natural products for their abilities to inhibit acetylcholinesterase and reverse scopolamine-induced amnesia. Among the plants tested Evodia rutaecarpa Bentham showed a strong inhibitory effect on acetylcholinesterase in vitro and an anti-amnesic effect in vivo. By sequential fractionation of E. rutaecarpa, the active component was finally identified as Dehydroevodiamine hydrochloride (DHED). DHED inhibited acetylcholinesterase activity in a dose-dependent and non-competitive manner. The IC50 value of DHED is 37.8 microM. A single administration of DHED to rats (6.25 mg/kg) significantly reversed the scopolamine-induced memory impairment in a passive avoidance test. The antiamnesic effect of DHED was more potent than that of tacrine which is the only drug for Alzheimer's disease approved by FDA. This potent antiamnesic effect of DHED was thought to be due to the combined effects of acetylcholinesterase inhibition and the known cerebral blood flow enhancement. These results indicate that DHED has novel anti-cholinesterase and antiamnesic activities and might have therapeutic potential in various disorders including Alzheimer's disease.