CRF, bovine
(Synonyms: CRF,CRH,促肾上腺皮质激素释放激素,Corticotropin Releasing Factor bovine) 目录号 : GC35748
CRF, bovine 是一种重要由41个氨基酸组成的神经肽，由下丘脑产生并运输到垂体并触发促肾上腺皮质激素（ACTH）的释放。
Cas No.:92307-52-3
Sample solution is provided at 25 µL, 10mM.
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CRF, bovine is an important neuropeptide consisting of 41 amino acids[1], it is produced by the hypothalamus and is transported to the pituitary, where it triggers the release of adrenocorticotropic hormone (ACTH)[2]. CRF, bovine plays a significant role in social behavior and is commonly utilized in research of various psychiatric disorders, such as depression, anxiety, and substance/alcohol abuse[3][4].
CRF, bovine (0.1µM; 3h) treatment can rapidly stimulated cyclic AMP production, increasing it 4- to 6-fold in 3min and reaching a peak of 10- to 15-fold at 30min in rat anterior pituitary cells[5]. In RAW 264.7 cells, treatment with CRF, bovine at 10nM for 4 hours enhanced the NF-κB-mediated luciferase expression[6].
CRF, bovine play important roles in sociability, intracerebroventricular administration of CRF, bovine (5µg/2µL; 30min) significantly decreased the number of entries and the time of interaction with the unknown male mice[7]. CRF (100ng; icv) consistently decreased immobility in the tail suspension test (TST) and the forced swim test (FST) in unshocked mice[8].
References:
[1]. Vale W, Spiess J, Rivier C, Rivier J. Characterization of a 41-residue ovine hypothalamic peptide that stimulates secretion of corticotropin and beta-endorphin. Science. 1981 Sep 18;213(4514):1394-7. doi: 10.1126/science.6267699. PMID: 6267699.
[2]. Hogg JE, Myers J, Hutson PH. The human neuroblastoma cell line, IMR-32, expresses functional corticotropin-releasing factor receptors. Eur J Pharmacol. 1996 Sep 26;312(2):257-61. doi: 10.1016/0014-2999(96)00470-0. PMID: 8894604.
[3]. Matsoukas MT, Panagiotopoulos V, Karageorgos V, et al. Structural and Functional Insights into CRF Peptides and Their Receptors. Biology (Basel). 2024 Feb 13;13(2):120. doi: 10.3390/biology13020120. PMID: 38392338; PMCID: PMC10886364.
[4]. Sanders J, Nemeroff C. The CRF System as a Therapeutic Target for Neuropsychiatric Disorders. Trends Pharmacol Sci. 2016 Dec;37(12):1045-1054. doi: 10.1016/j.tips.2016.09.004. Epub 2016 Oct 4. PMID: 27717506; PMCID: PMC5121012.
[5]. Aguilera G, Harwood JP, Wilson JX, et al. Mechanisms of action of corticotropin-releasing factor and other regulators of corticotropin release in rat pituitary cells. J Biol Chem. 1983 Jul 10;258(13):8039-45. PMID: 6305967.
[6]. Smith EM, Gregg M, Hashemi F, et al. Corticotropin Releasing Factor (CRF) activation of NF-kappaB-directed transcription in leukocytes. Cell Mol Neurobiol. 2006 Jul-Aug;26(4-6):1021-36. doi: 10.1007/s10571-006-9040-1. Epub 2006 Apr 22. PMID: 16633893; PMCID: PMC11520635.
[7]. Bagosi Z, Karasz G, Czébely-Lénárt A, et al. The effects of CRF and urocortins on the sociability of mice. Brain Res. 2017 May 15;1663:114-122. doi: 10.1016/j.brainres.2017.03.003. Epub 2017 Mar 14. PMID: 28315311.
[8]. Swiergiel AH, Leskov IL, Dunn AJ. Effects of chronic and acute stressors and CRF on depression-like behavior in mice. Behav Brain Res. 2008 Jan 10;186(1):32-40. doi: 10.1016/j.bbr.2007.07.018. Epub 2007 Jul 20. PMID: 17716752.
CRF, bovine 是一种重要由41个氨基酸组成的神经肽[1]，由下丘脑产生并运输到垂体并触发促肾上腺皮质激素（ACTH）的释放[2]。
CRF, bovine 在社交行为中发挥重要作用，并常用于抑郁症、焦虑症和物质/酒精滥用等多种精神疾病的研究[3][4]。CRF, bovine（0.1µM；3小时）处理可以迅速刺激cAMP的产生，在3分钟内增加4到6倍，并在30分钟内达到10到15倍的峰值[5]。在RAW 264.7细胞中，用10nM的CRF, bovine 处理4小时可以增强NF-κB介导的荧光素酶表达[6]。
CRF, bovine 在社交行为中扮演重要角色，脑室内注射CRF, bovine（5µg/2µL；30分钟）显著减少了小鼠进入次数和与陌生雄性互动的时间[7]。脑室内注射100ng的CRF在未受冲击的小鼠中一致减少了尾悬测试（TST）和强迫游泳测试（FST）中的不动时间[8]。
| Cell experiment [1]: | |
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Cell lines |
Rat anterior pituitary cells |
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Preparation Method |
Rat anterior pituitary cells were treated with 0.1µM CRF, bovine for 3h. Then the cyclic AMP content was measured. For cyclic AMP measurement, cells were incubated for 30min with 1mM methylisobutylxanthine. Cell suspensions were boiled in equal volumes of PBS for 10min, then cooled and centrifuged at 1500g for 20min, the cyclic AMP content of the supernatant was measured by radioimmunoassay. |
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Reaction Conditions |
0.1µM; 3h |
|
Applications |
CRF, bovine treatment can rapidly stimulated cyclic AMP production in rat anterior pituitary cells. |
| Animal experiment [2]: | |
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Animal models |
Male CFLP mice |
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Preparation Method |
The mice were implanted with a stainless steel Luer cannula aimed at the right lateral cerebral ventricle, the mice were allowed for 5 days to recover after the surgery then treated with CRF, bovine. Thirty minutes after ICV injection of the CRF, bovine mice were investigated in a social interaction test arena. |
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Dosage form |
5µg/2µL; 30min; icv. |
|
Applications |
CRF, bovine decreased the number of entries and the time of interaction with the unknown male. |
|
References: |
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| Cas No. | 92307-52-3 | SDF | |
| 别名 | CRF,CRH,促肾上腺皮质激素释放激素,Corticotropin Releasing Factor bovine | ||
| 分子式 | C206H340N60O63S | 分子量 | 4697.34 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.2129 mL | 1.0644 mL | 2.1289 mL |
| 5 mM | 0.0426 mL | 0.2129 mL | 0.4258 mL |
| 10 mM | 0.0213 mL | 0.1064 mL | 0.2129 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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Quality Control & SDS
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- Purity: >98.00%
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