Coniferin
(Synonyms: 松柏苷; Laricin) 目录号 : GC35725
A phenylpropanoid glycoside with diverse biological activities
Cas No.:531-29-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Coniferin is a phenylpropanoid glycoside and a lignan precursor that has been found in V. album and has diverse biological activities.1,2,3 It inhibits ADP-induced platelet aggregation in isolated human platelet-rich plasma when used at concentrations ranging from 0.01 to 1 ?M.1 Coniferin has antioxidant activity in an oxygen radical absorbance capacity (ORAC) assay.2 It induces contractions in isolated rat aortic rings when used at concentrations ranging from 0.001 to 1 mM.3 In vivo, coniferin (1 mg/kg) increases blood pressure in spontaneously hypertensive rats.4
1.Panossian, A., Kocharian, A., Matinian, K., et al.Pharmacological activity of phenylpropanoids of the mistletoe, Viscum album L., host: Pyrus caucasica Fed.Phytomedicine5(1)11-17(1998) 2.Kayano, S., Kikuzaki, H., Ikami, T., et al.A new bipyrrole and some phenolic constituents in prunes (Prunus domestica L.) and their oxygen radical absorbance capacity (ORAC)Biosci. Biotechnol. Biochem.68(4)942-944(2004) 3.Deliorman, D., ?ali?, ?., Ergun, F., et al.Studies on the vascular effects of the fractions and phenolic compounds isolated from Viscum album ssp. albumJ. Ethnopharmacol.72(1-2)323-329(2000) 4.Sawabe, A., Kumamoto, H., and Matsubara, Y.Bioactive glycosides in citrus fruit peelsBull. Inst. Compr. Agr. Sci. Kinki Univ.657-67(1998)
| Cas No. | 531-29-3 | SDF | |
| 别名 | 松柏苷; Laricin | ||
| Canonical SMILES | COC(C=C(/C=C/CO)C=C1)=C1O[C@@H]2O[C@@H]([C@@H](O)[C@H](O)[C@H]2O)CO.[E] | ||
| 分子式 | C16H22O8 | 分子量 | 342.34 |
| 溶解度 | DMSO: 10 mM | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.9211 mL | 14.6054 mL | 29.2107 mL |
| 5 mM | 0.5842 mL | 2.9211 mL | 5.8421 mL |
| 10 mM | 0.2921 mL | 1.4605 mL | 2.9211 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Distribution of Coniferin in freeze-fixed stem of Ginkgo biloba L. by cryo-TOF-SIMS/SEM
Sci Rep 2016 Aug 11;6:31525.PMID:27510918DOI:10.1038/srep31525.
To clarify the role of Coniferin in planta, semi-quantitative cellular distribution of Coniferin in quick-frozen Ginkgo biloba L. (ginkgo) was visualized by cryo time-of-flight secondary ion mass spectrometry and scanning electron microscopy (cryo-TOF-SIMS/SEM) analysis. The amount and rough distribution of Coniferin were confirmed through quantitative chromatography measurement using serial tangential sections of the freeze-fixed ginkgo stem. The lignification stage of the sample was estimated using microscopic observations. Coniferin distribution visualized at the transverse and radial surfaces of freeze-fixed ginkgo stem suggested that Coniferin is stored in the vacuoles, and showed good agreement with the assimilation timing of Coniferin to lignin in differentiating xylem. Consequently, it is suggested that Coniferin is stored in the tracheid cells of differentiating xylem and is a lignin precursor.
NaCl dependent production of Coniferin in Alluaudiopsis marnieriana suspension cultured cells
Plant Biotechnol (Tokyo) 2021 Mar 25;38(1):183-186.PMID:34177341DOI:10.5511/plantbiotechnology.21.0102a.
A stable salt-tolerant cell-suspension culture of Alluaudiopsis marnieriana was established, and intracellular compounds that accumulated under salt-stress conditions were investigated. HPLC/MS, and NMR analyses indicated that enhanced accumulation of Coniferin was found during the growth phase in medium containing 150 mM NaCl. Coniferin or its derivatives may play an important role in salt-tolerance mechanisms in this plant.
Distribution of Coniferin in differentiating normal and compression woods using MALDI mass spectrometric imaging coupled with osmium tetroxide vapor treatment
Tree Physiol 2016 May;36(5):643-52.PMID:26507270DOI:10.1093/treephys/tpv116.
Matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) was employed to detect monolignol glucosides in differentiating normal and compression woods of two Japanese softwoods, Chamaecyparis obtusa and Cryptomeria japonica Comparison of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry collision-induced dissociation fragmentation analysis and structural time-of-flight (MALDI-TOF CID-FAST) spectra between Coniferin and differentiating xylem also confirmed the presence of Coniferin in differentiating xylem. However, as matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) and MALDI-TOF CID-FAST spectra of sucrose were similar to those of Coniferin, it was difficult to distinguish the distribution of Coniferin and sucrose using MALDI-MSI and collision-induced dissociation measurement only. To solve this problem, osmium tetroxide vapor was applied to sections of differentiating xylem. This vapor treatment caused peak shifts corresponding to the introduction of two hydroxyl groups to the C=C double bond in Coniferin. The treatment did not cause a peak shift for sucrose, and therefore was effective in distinguishing Coniferin and sucrose. Thus, it was found that MALDI-MSI combined with osmium tetroxide vapor treatment is a useful method to detect Coniferin in differentiating xylem.
Coniferin dimerisation in lignan biosynthesis in flax cells
Phytochemistry 2007 Nov-Dec;68(22-24):2744-52.PMID:17988697DOI:10.1016/j.phytochem.2007.09.016.
[(13)C(2)]-Coniferin was provided to a flax (Linum usitatissimum L.) cell suspension to monitor subsequent dimerisation by MS and NMR. The label was mainly incorporated into a 8-8'-linked lignan, lariciresinol diglucoside, a 8-5'-linked neolignan, dehydrodiconiferyl alcohol glucoside and a diastereoisomeric mixture of a 8-O-4'-linked neolignan, guaiacylglycerol-beta-coniferyl alcohol ether glucoside. This latter compound is reported for the first time in flax. The strong and transient increase in these compounds in fed cells was concomitant with the observed peak in Coniferin content. These results suggest (i) a rapid metabolisation of Coniferin into lignans and neolignans and indicate the capacity of flax cells to operate different types of couplings, and (ii) a continuous synthesis and subsequent metabolisation of coniferin-derived dimers all over the culture period.
Unexpected behavior of Coniferin in lignin biosynthesis of Ginkgo biloba L
Planta 2005 Sep;222(1):58-69.PMID:15986215DOI:10.1007/s00425-005-1517-5.
To gain insight into the behavior of monolignol glucoside in Ginkgo biloba L., we examined glucosides potentially involved in lignin biosynthetic pathway. Coniferin (coniferyl alcohol 4O-beta-D-glucoside) is a strong candidate for the storage form of monolignol. Coniferaldehyde glucoside may also have a role in lignin biosynthesis; this was examined with tracer experiments using labeled glucosides fed to stem segments. A series of tracer experiments showed that Coniferin and coniferaldehyde glucoside were modified into coniferyl alcohol and then efficiently incorporated into lignin under the experimental conditions used. Interestingly, more than half of the administered Coniferin underwent an oxidation to the aldehyde form before its aglycone; coniferyl alcohol was polymerized into lignin. This suggests that there is an alternative pathway for Coniferin to enter the monolignol biosynthetic pathway, in addition to the direct pathway beginning with the deglucosylation of Coniferin catalyzed by beta-glucosidase. Enzymatic assays revealed that coniferaldehyde glucoside was produced enzymatically from Coniferin, and that coniferaldehyde glucoside can be deglucosylated to yield coniferaldehyde, which could be fated to become coniferyl alcohol . Albeit the findings cannot be taken as proof for the in-planta functioning, these results present a possibility for the existence of alternative pathway in which some of the stored Coniferin is oxidized to coniferaldehyde glucoside, which is deglucosylated to generate coniferaldehyde that joins the monolignol biosynthesis pathway.