Calceolarioside B
(Synonyms: 木通苯乙醇苷B) 目录号 : GC35581
Calceolarioside B (Nuomioside A), found in the herbs of Ajuga becumbens Thunb., has anti-proliferation property.Calceolarioside B exhibits significant inhibitory activity against rat lens aldose reductase (RLAR) with an IC50 of 23.99 μM. Calceolarioside B displays inhibitory effect on DPPH radical scavenging activity with an IC50 of 94.60 μM.
Cas No.:105471-98-5
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.50%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Calceolarioside B (Nuomioside A), found in the herbs of Ajuga becumbens Thunb., has anti-proliferation property.Calceolarioside B exhibits significant inhibitory activity against rat lens aldose reductase (RLAR) with an IC50 of 23.99 μM. Calceolarioside B displays inhibitory effect on DPPH radical scavenging activity with an IC50 of 94.60 μM.
| Cas No. | 105471-98-5 | SDF | |
| 别名 | 木通苯乙醇苷B | ||
| Canonical SMILES | O[C@H]([C@@H](O)[C@@H]1O)[C@@H](O[C@@H]1COC(/C=C/C2=CC(O)=C(O)C=C2)=O)OCCC3=CC(O)=C(O)C=C3 | ||
| 分子式 | C23H26O11 | 分子量 | 478.45 |
| 溶解度 | Soluble in DMSO | 储存条件 | -20°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0901 mL | 10.4504 mL | 20.9008 mL |
| 5 mM | 0.418 mL | 2.0901 mL | 4.1802 mL |
| 10 mM | 0.209 mL | 1.045 mL | 2.0901 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
[Determination of Calceolarioside B in Caulis Akebiae by RP-HPLC]
Zhongguo Zhong Yao Za Zhi 2007 Mar;32(6):476-8.PMID:17552147doi
Objective: To establish a quantitative method for Calceolarioside B in Caulis Akebiae. Method: The samples were separated at 35 degrees C on a Phenomenex Luna C18 column eluted with methanol-water-phosphoric acid (35: 65: 0.05) as mobile phase. Flow rate was at 1.0 mL x min(-1) and the detection wavelength was at 330 nm. Result: The calibration curve was linear over the range from 0. 04 to 0. 86 g (r = 0. 9998) and the average recovery was 97.2%. 39 Crude drug materials collected from different areas were determined and the contents of Calceolarioside B in Caulis Akebiae were fluctuated from 0. 021% to 0. 745%. Conclusion: The method is simple and repeatable and could be used for the quality control of Caulis Akebiae.
Three new naphthoquinones from the tubers of Sinningia mauroana
Nat Prod Res 2023 Jan;37(2):263-268.PMID:34498959DOI:10.1080/14786419.2021.1969564.
Three new naphthoquinones, 5,6,7-trimethoxydunnione (1), 6,7-dimethoxy-α-dunnione (2), and 5,6,7-trimethoxydunniol (3) were isolated from the tubers of Sinningia mauroana Chautems, together with eleven known compounds: 5-hydroxy-6,7-dimethoxy-α-dunnione (4), 6-hydroxy-7-methoxy-α-dunnione (5), 7-hydroxy-6-methoxy-α-dunnione (6), cedrol (7), tectoquinone (8), plantainoside A (9), calceolarioside A (10), Calceolarioside B (11), sanangoside (12), allo-calceolarioside A (13), and conandroside (14). The known compounds 4, 7-9 and 11-13 are being reported for the first time in this species. Hexane, ethyl acetate and ethanol extracts were tested for antioxidant activity by the ORAC-FL method, and the antioxidant capacity was measured as trolox equivalent (TE). The ethyl acetate (8808 µg TE g-1) and ethanol (7911 µg TE g-1) extracts showed high antioxidant capacity, while the hexane extract displayed weak antioxidant capacity (860 µg TE g-1).
HIV gp41 binding phenolic components from Fraxinus sieboldiana var. angustata
Planta Med 2002 Nov;68(11):1034-6.PMID:12451497DOI:10.1055/s-2002-35665.
By means of HIV gp41 binding affinity directed chromatographic fractionation, three phenylethanoid glycosides; calceolarioside A ( 1), Calceolarioside B ( 2) and acteoside ( 3), along with four hydroxycoumarins; esculin ( 4), fraxin ( 5), fraxetin ( 6) and esculetin ( 7), and one lignan, (-)-olivil ( 8) were isolated from the n-butyl alcohol fraction of Fraxinus sieboldiana var. angustata. Among the isolated compounds, Calceolarioside B ( 2) and esculetin ( 7) showed moderate binding affinity on HIV gp41 with IC 50 values of 0.1 mg/ml and 0.5 mg/ml, respectively. Calceolarioside A ( 1), Calceolarioside B ( 2), acteoside ( 3), and (-)-olivil ( 8) were isolated for the first time from this plant.
Identification of the functional food ingredients with antithrombotic properties via virtual screen and experimental studies
Food Chem 2021 Nov 15;362:130237.PMID:34091163DOI:10.1016/j.foodchem.2021.130237.
Thrombin is a key therapeutic target protein of thrombosis. To date, massive studies have focused on the exploration of antithrombotic compounds. Here we capitalize on molecular docking, molecular simulations and spectroscopic experiments for virtually screening natural products that can inhibit thrombin and elucidating their interaction mechanism. Six compounds are screened from a natural product database by a cross-analysis based on two semi-flexible molecular docking methods. We show that four compounds can effectively inhibit thrombin and Calceolarioside B is the most competitive one based on enzyme inhibition experiments. Moreover, the binding free energies of these compounds with thrombin exhibit a consistent rank trend with their enzyme inhibition assay results. In addition, the Van der Waals is the main force to drive the interaction between the ligands and the receptor, which can be deduced from the fluorescence spectral results. This work provides a new insight into the development of antithrombotic natural compounds.
A deep clustering-based mass spectral data visualization strategy for anti-renal fibrotic lead compound identification from natural products
Analyst 2022 Oct 24;147(21):4739-4751.PMID:36155672DOI:10.1039/d2an01185a.
Natural products have been a key source of drug lead discovery. However, their identification has long been a challenge even with the state-of-the-art analysis technologies like high-resolution mass spectrometry (MS) due to their complexity. Emerging in silico chemical structure prediction tools have provided time-saving and highly efficient approaches for identification of these complex samples. Nevertheless, the interpretation of these MS annotations into key supporting evidence towards specific questions is still a bottleneck in medicinal and biological fields. Here we present a deep clustering-based MS data visualization strategy (MCnebula), integrated with the influential open-source automatic MS annotation platform SIRIUS and in vivo and in vitro methods, to screen and validate potential lead compounds from natural products. MCnebula could provide multi-layer clustering profiles with chemical ontologies and comparative analysis of differential treatments. Plantaginis Semen (PS) is commonly used for treating kidney disease and usually stir-fried with salt water to enhance its anti-renal fibrosis effect, but the reason behind this remains unclear. Taking PS as an example, we comprehensively identified and compared the raw and processed PS extracts with SIRIUS-MCnebula, and screened potential anti-renal fibrotic lead compounds using weighted fold change analysis. Eighty-nine components were identified in PS with isoacteoside, Calceolarioside B, 2'-acetylacteoside, and plantainoside D being screened and validated to treat renal fibrosis. The novel developed mass spectral data visualization strategy combined with biological function investigation and validation workflow could not only accelerate the discovery of lead compounds from medicinal natural products, but also shed new light on the traditional processing theory.