Artemitin
(Synonyms: 艾黄素) 目录号 : GC35400
Artemitin, a significant flavonol compound existing in Laggera pterodonta (DC.) Benth., Artemisia rupestris L, etc., possesses bioactivities of antioxidative, anti-inflammatory and antiviral.
Cas No.:479-90-3
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Artemitin, a significant flavonol compound existing in Laggera pterodonta (DC.) Benth., Artemisia rupestris L, etc., possesses bioactivities of antioxidative, anti-inflammatory and antiviral.
[1] Han X, et al. Biomed Chromatogr. 2018 Dec;32(12):e4356.
| Cas No. | 479-90-3 | SDF | |
| 别名 | 艾黄素 | ||
| Canonical SMILES | O=C1C2=C(O)C(OC)=C(OC)C=C2OC(C3=CC(OC)=C(OC)C=C3)=C1OC | ||
| 分子式 | C20H20O8 | 分子量 | 388.37 |
| 溶解度 | Soluble in DMSO | 储存条件 | 4°C, protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.5749 mL | 12.8743 mL | 25.7486 mL |
| 5 mM | 0.515 mL | 2.5749 mL | 5.1497 mL |
| 10 mM | 0.2575 mL | 1.2874 mL | 2.5749 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Development of an LC-MS/MS-based assay to determine Artemitin in rat plasma and its application in a pharmacokinetic study
Biomed Chromatogr 2018 Dec;32(12):e4356.PMID:30073671DOI:10.1002/bmc.4356.
Artemitin, a significant flavonol compound existing in Laggera pterodonta (DC.) Benth., Artemisia rupestris L, etc., is the subject of attention by researchers owing to its pharmacological activities (such as antioxidative, anti-inflammatory and antiviral). In this work, a highly sensitive and specific high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (HPLC-ESI-MS/MS) assay combined with protein precipitation has been established and validated for determining Artemitin concentration in rat plasma. Both Artemitin and warfarin sodium (internal standard, IS) were separated on an Agela Venusil XBP Phenyl column through the isocratic elution mode of methanol-water containing 0.1% formic acid (80:20, v/v), at a flow rate of 0.4 mL/min. The MS/MS system was operated in a positive ion and ESI multiple reaction monitoring mode, and the multiple reaction monitoring transition was optimized as m/z 389.0 → 373.0 for Artemitin and 309.2 → 163.0 for IS. The method showed good linearity in the range of 2.5-2000 ng/mL (R2 = 1.0000) and high sensitivity for Artemitin with the lower limit of quantification of 2.5 ng/mL. The intra- and inter-day accuracies were 97.4-100.9 and 93.4-100.3%, respectively. The intra- and inter-day precisions were <4.8 and 6.5%, respectively. The extraction efficiency and absolute recovery were >66.5 and 71.3%, respectively. In addition, a good matrix effect of <9.5% was obtained. As a result, the method developed herein was successfully applied for the pharmacokinetic study of Artemitin after an intravenous administration in rats.
[Chemical constituents from Laggera pterodonta]
Zhong Yao Cai 2014 May;37(5):816-9.PMID:25335290doi
Objective: To study the chemical constituents from Laggera pterodonta. Methods: The compounds were isolated and purified by column chromatographic methods with silica gel, Sephadex LH-20, ODS and preparation HPLC. Their structures were identified by physicochemical properties and spectral data. Results: Ten compounds were isolated and elucidated as 3,4',5-trihydroxy-6,7-dimethoxyflavone (1), 3, 3', 5-trihydroxy-4', 6,7-trimethoxyflavone (2), chrysosplenetin B (3), 5-hydroxy-4', 7-dimethoxyflavanone (4), 5, 7,4'-trihydroxy-3, 3'-dimethoxyflavone (5), Artemitin (6), quercetin (7), pinostrobin (8), luteolin (9) and apigenin (10), respectively. Conclusion: Compounds 1, 2, 4, 5 and 8 - 10 are isolated from this plant for the first time.
[Chemical constituents of Laggera pterodonta]
Zhongguo Zhong Yao Za Zhi 2010 Mar;35(5):602-6.PMID:20506820DOI:10.4268/cjcmm20100513.
Objective: To study the chemical constituents of Laggera pterodonta. Method: The ethanol extract of L. pterodonta was isolated by column chromatogramphy on silica gel, ODS, and Sephadex LH-20 to afford compounds. The structures of the obtained compounds were identified by chemical reactions and spectroscopic analysis. Result: Nineteen compounds were separated and identified to be pterodondiol (1), ilicic acid (2), Artemitin (3), chrysosplenetin B (4), 3,5-dihydroxy-3',4',6,7-tetramethoxyflavone (5), chrysosplenol D (6), 5,6,4'-trihydroxy-3,7-dimethoxyflavone (7), quercetin (8), tamarixetin (9), patuletin (10), quercetin-3-O-beta-D-galactopyranoside (11), patuletin-3-O-beta-D-glucopyranoside (12), helichrysoside (13), 4,5,7-trihydroxy-6-methoxyflavone-3-O-beta-D-rutinoside (14), kaempferol-3-O-beta-D-glucopyranoside (15), stigmasterol (16), stigmasterol 3-O-beta-D-glucopyranoside (17), 2-hydroxy-benzoic acid (18), beta-sitosterol (19). Conclusion: Compounds 5, 7, 9-15, and 17-18 were isolated from this plant for the first time. The 13C-NMR data of compound 7 is reported for the first time.
Terpenoids and flavonoids from Laggera pterodonta
Yao Xue Xue Bao 2007 May;42(5):511-5.PMID:17703774doi
To study the chemical constituents of aerial parts of Laggera pterodonta (DC.) Benth., the air-dried aerial parts of this plant were powered and extracted with boiling water and purified by silica gel column chromatography and recrystallized. Eleven compounds were obtained from L. pterodonta. They were identified as to be 6-O-beta-D-glucopyranosyl-carvotanacetone (1), pterodontic acid (2), 1beta-hydroxy pterondontic acid (3), pterodontoside A (4), pterodondiol (5), pterodontriol B (6), 5-hydroxy-3,4', 6,7-tetramethoxyflavone (7), Artemitin (8), chrysosplenetin B (9), quercetin (10) and beta-sitosterol (11). Compound 1 is a new monoterpene glucoside. Compounds 10 and 11 were isolated from this plant for the first time. Compounds 2 and 5 showed moderate activity against bacteria including Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis, Mycobacteium phlei and Bacillus circulans by paper disc diffusion method, while they both displayed no activity against Escherichia coli.
Chemical constituents of Artemisia arborescens and the effect of the aqueous extract on rat isolated smooth muscle
Planta Med 1995 Jun;61(3):242-5.PMID:7617767DOI:10.1055/s-2006-958064.
Phytochemical analysis of the ground aerial parts of Artemisia arborescens resulted in the isolation from the ethanolic extract of the known compounds: Artemitin, arborescin, sesamin, (+)-lirioresinol beta-dimethyl ether, chrysoeriol, apigenin, beta-sitosteryl glucoside, dihydroridentin, and chrysoeriol 4-glucoside. The last six compounds are isolated from this plant for the first time. The same fraction also yielded the new eudesmanolide jordanolide (1). The effect of an aqueous extract of the plant was studied on rat isolated ileum, uterus, and urinary bladder. The aqueous extract (AE) caused a concentration-dependent reduction in the amplitude of the phasic contractions and in the tone of the ileum. On the other hand, AE caused a significant increase in the frequency as well as the amplitude of the phasic contractions and increased the tone of the isolated uterus and the urinary bladder strips. On the uterus, quinacrine, an inhibitor of the release of arachidonic acid and its metabolites, and indomethacin, a cyclooxygenase inhibitor, potentiated rather than inhibited the effects of AE on this tissue. These observations are discussed in relation to the use of the plant extract in folk medicine.