Glycerol phosphate oxidase(BR, ≥30units/mg)

Glycerol phosphate oxidase(BR，10units/mg solid) is a flavin oxidase that uses sn-glycerol-3-phosphate as a substrate and catalyzes its production of DHAP and H₂O₂ ^[1]. Glycerol phosphate oxidase converts glycerol-3-phosphate into dihydroxyacetone phosphate and produces hydrogen peroxide, which is essential for energy production and balanced redox signaling in cells ^[2]. Glycerol phosphate oxidase ^[3]: At pH 6.5 and 37℃, the amount of enzyme required to oxidize 1 micromole of L-α-glycerophosphate per minute is one unit of activity. The molecular weight of the enzyme is 67kDa (determined by SDS-PAGE) and the isoelectric point is 4.6. The optimum pH is 6.0 and the optimum reaction temperature is 45-55℃. It is stable for 20 hours in the pH 5.0-8.0 range at 25℃ and its thermal stability is below 40℃ (15 minutes at pH 6.5). The enzyme is affected by a variety of inhibitors, including Cu²⁺, Fe³⁺, Zn²⁺, EDTA, Proclin, and SDS.

References:
[1]. Gancedo C, Gancedo JM, Sols A. Glycerol metabolism in yeasts: pathways of utilization and production. European journal of biochemistry. 1968 Jul; 5(2): 165-172.
[2]. Koga Y, Konishi K, Kobayashi A, et al. Anaerobic glycerol-3-phosphate dehydrogenase complex from hyperthermophilic archaeon Thermococcus kodakarensis KOD1. Journal of bioscience and bioengineering. 2019 Jun 1; 127(6): 679-685.
[3]. Yan C, Jin K, Luo X, et al. Electrochemical Biosensor Based on Chitosan-and Thioctic-Acid-Modified Nanoporous Gold Co-Immobilization Enzyme for Glycerol Determination. Chemosensors. 2022 Jul 2; 10(7): 258.

Glycerol phosphate oxidase(BR，10units/mg solid)是一种以sn-glycerol-3-phosphate为底物、催化其生成DHAP和H₂O₂的黄素氧化酶 ^[1]。Glycerol phosphate oxidase将glycerol-3-phosphate转化为磷酸二羟基丙酮和产生过氧化氢，对于细胞中的能量产生和平衡氧化还原信号传导至关重要 ^[2]。Glycerol phosphate oxidase ^[3]：在pH 6.5和37℃下，每分钟氧化1微摩尔L-α-甘油磷酸所需的酶量为一个酶活单位。该酶的分子量为67kDa（经SDS-PAGE测定），等电点为4.6。最适pH为6.0，最适反应温度为45-55℃。在25℃、pH 5.0-8.0范围内，该酶可稳定反应20小时，在40℃以下（pH 6.5时可稳定反应15分钟）热稳定性为15分钟。该酶受多种抑制剂的影响，包括Cu²⁺、Fe³⁺、Zn²⁺、EDTA、Proclin和SD。