pTH-Related Protein (1-34) (human, mouse, rat)
目录号 : GA23412
pTH-Related Protein (1-34) (human, mouse, rat)是一种在人、小鼠和大鼠物种间具有保守序列的肽。pTH-Related Protein (1-34)与甲状旁腺激素（PTH）具有显著的同源性，并与相同的PTH受体结合，参与多种生理过程。
Cas No.:112540-82-6
Sample solution is provided at 25 µL, 10mM.
_1-3.$$$39978408@51.jpg');)
_1-9.$$$38538795@65.jpg');)
_1-9.$$$39112701@51.jpg');)
_1-7.$$$37316672@70.jpg');)
_366-372.$$$36198801@70.jpg');)
.$$$38565142@65.jpg');)
_1867-1883.$$$33248023@67.jpg');)
_eads6055.$$$39977546@45.jpg');)
_eadm9805.$$$40080571@45.jpg');)
_eadj3020.$$$39666821@45.jpg');)
_1-20.$$$39757301@28.jpg');)
_1-24.$$$38898113@28.jpg');)
_273-287.$$$36806353@46.jpg');)
_1-16.$$$37156877@44.jpg');)
_1-19.$$$37460805@44.jpg');)
_1291-1307.$$$34518654@46.jpg');)
pTH-Related Protein (1-34) (human, mouse, rat) is a peptide with a conserved sequence among human, mouse, and rat species. pTH-Related Protein (1-34) shares significant homology with parathyroid hormone (PTH) and binds to the same PTH receptor, participating in various physiological processes[1]. In the regulation of calcium and phosphate metabolism, pTH-Related Protein (1-34) acts on the kidneys and bones. In the kidneys, pTH-Related Protein (1-34) promotes calcium reabsorption and reduces phosphate reabsorption, helping to maintain appropriate levels of calcium and phosphate in the blood[2]. In bone tissue, pTH-Related Protein (1-34) initially stimulates osteoblast activity, promoting bone formation. However, persistent and excessive activation of pTH-Related Protein (1-34) may also lead to bone resorption by indirectly activating osteoclasts[3]. pTH-Related Protein (1-34) also affects cell proliferation. In some cancer cells, abnormal expression of pTH-Related Protein (1-34) may contribute to tumor growth and metastasis[4].
In vitro, pTH-Related Protein (1-34) (10nM) was used to treat UMR106-01 cells (an osteoblastic cell line) by brief exposure (12 minutes) followed by washout, inducing sustained cAMP generation and persistent activation of the cAMP/PKA signaling pathway, as well as sustained upregulation of several CREB-responsive genes (c-Fos, IL-6, RANKL, Bglap1)[5]. pTH-Related Protein (1-34) (human, mouse, rat) was also used at a concentration of 10nM to treat the human breast cancer cell line MCF7. pTH-Related Protein (1-34) increased cell proliferation levels but did not alter p27 protein expression levels[6].
In vivo, pTH-Related Protein (1-34) (human, mouse, rat) (0.2µg/day) was administered via subcutaneous injection to 25-hydroxyvitamin D-1α-hydroxylase and PTH double knockout mice (starting from postnatal day 4 for 10 days or up to 1 month). pTH-Related Protein (1-34) significantly increased serum calcium levels in the mice, alleviated severe hypocalcemia, and prolonged survival. pTH-Related Protein (1-34) enhanced renal calcium reabsorption by increasing renal calcium transport protein expression, leading to increased long bone length, enlarged epiphyseal volume, enhanced proliferation and differentiation of growth plate chondrocytes, and increased cartilage matrix mineralization[7]. Four-month-old male C57Bl/6J mice were given subcutaneous injections of pTH-Related Protein (1-34) (human, mouse, rat) (80µg/kg/day) for 6 weeks, resulting in increased mineral density in the tooth roots of the mice[8].
References:
[1] Suva LJ, Winslow GA, Wettenhall RE, et al. A parathyroid hormone-related protein implicated in malignant hypercalcemia: cloning and expression. Science. 1987 Aug 21;237(4817):893-6.
[2] Chen T, Wang Y, Hao Z, et al. Parathyroid hormone and its related peptides in bone metabolism. Biochem Pharmacol. 2021 Oct;192:114669.
[3] Mundy GR. Metastasis to bone: causes, consequences and therapeutic opportunities. Nat Rev Cancer. 2002 Aug;2(8):584-93.
[4] Johnson RW, Rhoades J, Martin TJ. Parathyroid hormone-related protein in breast cancer bone metastasis. Vitam Horm. 2022;120:215-230.
[5] Ho PWM, Chan AS, Pavlos NJ, et al. Brief exposure to full length parathyroid hormone-related protein (PTHrP) causes persistent generation of cyclic AMP through an endocytosis-dependent mechanism. Biochem Pharmacol. 2019 Nov;169:113627.
[6] Edwards CM, Kane JF, Smith JA, et al. PTHrP intracrine actions divergently influence breast cancer growth through p27 and LIFR. Breast Cancer Res. 2024 Feb 26;26(1):34.
[7] Xue Y, Zhang Z, Karaplis AC, et al. Exogenous PTH-related protein and PTH improve mineral and skeletal status in 25-hydroxyvitamin D-1alpha-hydroxylase and PTH double knockout mice. J Bone Miner Res. 2005 Oct;20(10):1766-77.
[8] Hsu C, He Z, Le Henaff C, et al. Differential effects of parathyroid hormone, parathyroid hormone-related protein, and abaloparatide on collagen 1 expression by mouse cementoblasts and mouse tooth root density. Am J Orthod Dentofacial Orthop. 2023 Mar;163(3):378-388.
pTH-Related Protein (1-34) (human, mouse, rat)是一种在人、小鼠和大鼠物种间具有保守序列的肽。pTH-Related Protein (1-34)与甲状旁腺激素（PTH）具有显著的同源性，并与相同的PTH受体结合，参与多种生理过程[1]。在钙磷代谢调节方面，pTH-Related Protein (1-34)作用于肾脏和骨骼。在肾脏中，pTH-Related Protein (1-34)促进钙的重吸收并减少磷的重吸收，有助于维持血液中适当的钙磷水平[2]。在骨组织中，早期pTH-Related Protein (1-34)刺激成骨细胞活性，促进骨形成。然而，持续和过度的pTH-Related Protein (1-34)激活也可能通过间接激活破骨细胞导致骨吸收 [3]。pTH-Related Protein (1-34)还对细胞增殖有影响。在一些癌细胞中，pTH-Related Protein (1-34)异常表达可能有助于肿瘤的生长和转移[4]。
在体外，pTH-Related Protein (1-34) (human, mouse, rat)（10nM）通过短暂暴露（12分钟）后洗脱的方式处理UMR106-01细胞（一种成骨细胞系），诱导了cAMP的持续生成，还导致了cAMP/PKA信号通路的持续激活，持续激活以及多个CREB响应基因（c-Fos、IL-6、RANKL、Bglap1）的持续上调 [5]。pTH-Related Protein (1-34) (human, mouse, rat)以10 nM的浓度处理人乳腺癌细胞系MCF7。pTH-Related Protein (1-34)增加了细胞的增殖水平但不引起p27蛋白表达水平变化[6]。
在体内，pTH-Related Protein (1-34) (human, mouse, rat)（0.2µg/天）通过皮下注射给予25-羟维生素D-1α-羟化酶和pTH双敲除小鼠（从出生后第4天开始，持续10天或至1个月）。pTH-Related Protein (1-34)显著提高了小鼠的血清钙水平，改善了严重的低钙血症，并延长了小鼠的生存时间，pTH-Related Protein (1-34)通过增加肾钙转运蛋白的表达，促进了肾钙重吸收，长骨长度增加、骨骺体积增大、生长板软骨细胞增殖和分化增强，以及软骨基质矿化增加[7]。4个月大的雄性C57Bl/6J小鼠接受皮下注射pTH-Related Protein (1-34) (human, mouse, rat)（80μg/kg/天），持续6周，pTH-Related Protein (1-34)增加了小鼠牙齿根部的矿化密度[8]。
| Cell experiment [1]: | |
|
Cell lines |
UMR106-01 cells |
|
Preparation Method |
UMR106-01 cells were seeded into 12-well plates with an appropriate number of cells per well and cultured for a sufficient period to reach confluence. Cells were treated with PTH-Related Protein (1-34) at various concentrations (10nM, 100nM) for 12 minutes. |
|
Reaction Conditions |
PTH-Related Protein (1-34), 10nM, 100nM; 12 minutes |
|
Applications |
PTH-Related Protein (1-34) induced a transient increase in cAMP formation in UMR106-01 cells. |
| Animal experiment [2]: | |
|
Animal models |
PTH−/−1α(OH)ase−/− mice (double knockout mice deficient in both PTH and 25-hydroxyvitamin D-1α-hydroxylase) |
|
Preparation Method |
Mice heterozygous for the PTH null allele and for the 1α(OH)ase null allele were mated to generate pups homozygous for both null alleles. pTH-Related Protein (1-34)(PTHrP) and PTH(1-86) were administered subcutaneously starting 4 days after birth for 10 days or until 1 month of age. Serum biochemistry and skeletal radiology, histology, and histomorphometry were performed. |
|
Dosage form |
0.2 µg/day PTHrP or PTH(1-86); s.c. |
|
Applications |
PTHrP increased serum calcium levels, improved hypocalcemia, prolonged survival, and increased renal calcium transporter expression |
|
References: |
|
| Cas No. | 112540-82-6 | SDF | |
| 分子式 | C180H287N57O48 | 分子量 | 4017.61 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.2489 mL | 1.2445 mL | 2.489 mL |
| 5 mM | 0.0498 mL | 0.2489 mL | 0.4978 mL |
| 10 mM | 0.0249 mL | 0.1245 mL | 0.2489 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet