L-Albizziine
(Synonyms: L-脲基丙氨酸) 目录号 : GA23127
L-Albizziine是一种4-亚甲基谷氨酰胺酶抑制剂，IC50值为20mM。
Cas No.:1483-07-4
Sample solution is provided at 25 µL, 10mM.
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L-Albizziine is an inhibitor of 4-methyleneglutaminase, with an IC50 value of 20mM [1]. L-Albizziine, an inhibitor of glutamate synthase, strongly inhibited the conversion of 2-ketoglutarate in maize leaves[2]. L-Albizziine inhibits asparagine synthetase activity and has been widely used to regulate T-cell activation in different cell models[3].
In vitro, L-Albizziine treatment at 2mM for 72 hours significantly reduced aspartate levels and increased p53 protein expression in HuCCT1 cells[4]. Treatment with 4mM L-Albizziine for 48 hours inhibited p53+/+ and p53−/− HCT116 cell proliferation and reduced cell number[5]. Treatment with 10mM L-Albizziine for 24h impaired vaccinia virus replication in primary human foreskin fibroblasts without affecting cell viability[6].
References:
[1] Ibrahim S A, Lea P J, Fowden L. Purification and properties of 4-methyleneglutaminase from the leaves of peanut (Arachis hypogaea)[J]. Phytochemistry, 1984, 23(8): 1545-1549.
[2] Harel E, Ne'Eman E. Alternative Routes for the Synthesis of 5-Aminolevulinic Acid in Maize Leaves: II. Formation from Glutamate[J]. Plant Physiology, 1983, 72(4): 1062-1067.
[3] Wu J, Li G, Li L, et al. Asparagine enhances LCK signalling to potentiate CD8+ T-cell activation and anti-tumour responses[J]. Nature cell biology, 2021, 23(1): 75-86.
[4] Chu Z, Zhang B, Zhou X, et al. A DNA/RNA heteroduplex oligonucleotide coupling asparagine depletion restricts FGFR2 fusion-driven intrahepatic cholangiocarcinoma[J]. Molecular Therapy Nucleic Acids, 2023, 34.
[5] Deng L, Yao P, Li L, et al. p53-mediated control of aspartate-asparagine homeostasis dictates LKB1 activity and modulates cell survival[J]. Nature communications, 2020, 11(1): 1755.
[6] Pant A, Cao S, Yang Z. Asparagine is a critical limiting metabolite for vaccinia virus protein synthesis during glutamine deprivation[J]. Journal of Virology, 2019, 93(13): 10.1128/jvi. 01834-18.
L-Albizziine是一种4-亚甲基谷氨酰胺酶抑制剂，IC50值为20mM[1]。作为谷氨酸合酶抑制剂，该化合物可强烈抑制玉米叶片中2-酮戊二酸的转化[2]。L-Albizziine通过抑制天冬酰胺合成酶活性，广泛应用于不同细胞模型中调控T细胞活化[3]。
在体外，2mM的L-Albizziine处理HuCCT1细胞72小时可显著降低天冬氨酸水平并增加p53蛋白表达[4]。4mM的L-Albizziine处理p53+/+和p53−/− HCT116细胞48小时能抑制细胞增殖并减少细胞数量[5]。10mM的L-Albizziine处理原代人包皮成纤维细胞24小时可抑制痘苗病毒复制且不影响细胞活力[6]。
| Cell experiment [1]: | |
Cell lines | HuCCT1 cells |
Preparation Method | HuCCT1 cells transfected with FGFR2-AHCYL1 (FA Cho-HDO) (200nM) were cultured in RPMI-1640 medium with or without L-Albizziine (2mM) or Asn (0.3mM) for 72h, medium supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, and cultures were incubated in a humidified atmosphere with 5% CO2. Cellular proteins were extracted and subjected to Western blot analysis using RIPA lysis buffer containing protease/phosphatase inhibitors. |
Reaction Conditions | 2mM; 72h |
Applications | L-Albizziine treatment inhibited asparagine synthase activity and increased p53 protein expression in HuCCT1 cells. |
References: | |
| Cas No. | 1483-07-4 | SDF | |
| 别名 | L-脲基丙氨酸 | ||
| 分子式 | C4H9N3O3 | 分子量 | 147.13 |
| 溶解度 | 50mg/ml in water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 6.7967 mL | 33.9836 mL | 67.9671 mL |
| 5 mM | 1.3593 mL | 6.7967 mL | 13.5934 mL |
| 10 mM | 679.7 μL | 3.3984 mL | 6.7967 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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