Fraxetin
(Synonyms: 弗拉西汀) 目录号 : GN10556
Fraxetin是一种植物来源的O-甲基香豆素。
Cas No.:574-84-5
Sample solution is provided at 25 µL, 10mM.
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Fraxetin is a plant-derived O-methylcoumarin [1]. Fraxetin exerts anti-inflammatory effects by directly scavenging reactive oxygen free radicals and activating antioxidant defenses, and intervening in inflammatory signals [2]. Fraxetin has anti-tumor, anti-inflammatory, and neuroprotective effects [3-4].
In MCF-7 cells, Fraxetin (10-60µM; 24h, 48h) significantly inhibited cell proliferation in a concentration and time-dependent manner [5]. In SKOV3 and SW626 cells, Fraxetin (80µM; 12h) suppresses the proliferation, migration, and invasion of ovarian cancer cells by inhibiting the TLR4/STAT3 signaling pathway [6]. In T98G and LN-229 cells, Fraxetin (5-50µM; 48h) inhibited cell viability in a concentration-dependent manner [7].
In C57BL/6J mice, Selenium-dependent glutathione reductase activity is significantly increased in the liver of Fraxetin (25mg/kg; po; 30d) treated mice [8]. In Dextran sulphate sodium (DSS)-induce a mouse model of colitis, Fraxetin (10mg/kg, 30mg/kg, 60mg/kg; po; 8d) alleviates DSS-induced colitis by modulating the inflammatory response, enhancing epithelial barrier integrity and regulating the gut microbiota [9].
References:
[1]. Wang H, Zou DA, Xie K, et al. Antibacterial mechanism of fraxetin against Staphylococcus aureus. Molecular Medicine Reports. 2014 Nov 1; 10(5): 2341-2345.
[2]. Balaha M, Ahmed N, Geddawy A, et al. Fraxetin prevented sodium fluoride-induced chronic pancreatitis in rats: Role of anti-inflammatory, antioxidant, antifibrotic and anti-apoptotic activities. International immunopharmacology. 2021 Apr 1; 93: 107372.
[3]. Ha NM, Son NT. Health benefits of fraxetin: from chemistry to medicine. Archiv der Pharmazie. 2024 Jul; 357(7): 2400092.
[4]. Fang T, Liu L, Liu W. Exploring the mechanism of fraxetin against acute myeloid leukemia through cell experiments and network pharmacology. BMC Complementary Medicine and Therapies. 2024 Jun 10; 24(1): 226.
[5]. Liu G, Liu Z, Yan Y, et al. Effect of fraxetin on proliferation and apoptosis in breast cancer cells. Oncology Letters. 2017 Dec 1; 14(6): 7374-7378.
[6]. Xu R, Ruan Y, Zhang L, et al. Fraxetin suppresses the proliferation, migration, and invasion of ovarian cancer cells by inhibiting the TLR4/STAT3 signaling pathway. Immunopharmacology and Immunotoxicology. 2023 May 4; 45(3): 287-294.
[7]. Yao H, Li X, Pan X, et al. Fraxetin exerts anticancer effect in glioma by suppressing MiR‐21‐3p. Drug Development Research. 2022 Apr; 83(2): 501-511.
[8]. MARTÍN‐ARAGÓN SA, Benedí JM, Villar AM. Modifications on antioxidant capacity and lipid peroxidation in mice under fraxetin treatment. Journal of pharmacy and pharmacology. 1997 Jan; 49(1): 49-52.
[9]. Sun X, Jin X, Wang L, et al. Fraxetin ameliorates symptoms of dextran sulphate sodium-induced colitis in mice. Heliyon. 2024 Jan 15; 10(1).
Fraxetin是一种植物来源的O-甲基香豆素 [1]。Fraxetin通过直接清除活性氧自由基、激活抗氧化防御机制以及干预炎症信号发挥抗炎作用 [2]。Fraxetin具有抗肿瘤、抗炎和神经保护作用 [3-4]。
在MCF-7细胞中，Fraxetin（10-60µM；24h、48h）以浓度和时间依赖性方式显著抑制细胞增殖 [5]。在SKOV3和SW626细胞中，Fraxetin（80µM；12h）通过抑制TLR4/STAT3信号通路抑制卵巢癌细胞的增殖、迁移和侵袭 [6]。在T98G和LN-229细胞中，Fraxetin（5-50µM；48h）以浓度依赖性方式抑制细胞活力 [7]。
在C57BL/6J小鼠中，Fraxetin（25mg/kg；po；30d）治疗组小鼠肝脏中硒依赖性谷胱甘肽还原酶活性显著升高 [8]。在葡聚糖硫酸钠（DSS）诱发的小鼠结肠炎模型中，Fraxetin（10mg/kg，30mg/kg，60mg/kg；po；8d）通过调节炎症反应、增强上皮屏障完整性和调节肠道菌群，减轻了DSS诱发的结肠炎 [9]。
| Cell experiment [1]: | |
Cell lines | MCF-7 cells |
Preparation Method | MCF-7 cells were seeded in 96-well plates at a concentration of 1×105/mL and each well contained 100µL. After 24h, Fraxetin was added to 10, 20, 40 and 60µM final concentrations. The control group was treated without Fraxetin. The cells were incubated for 24 and 48h at 37℃ in 5% CO2 and then culture medium was changed. |
Reaction Conditions | 10-60μM; 24h, 48h |
Applications | In MCF-7 cells, Fraxetin significantly inhibited cell proliferation in a concentration and time-dependent manner. |
| Animal experiment [2]: | |
Animal models | C57BL/6J mice |
Preparation Method | C57BL/6J male 12-month-old mice maintained under natural lighting conditions in a temperature controlled (22 ± 3℃) room and acclimatized to laboratory environment for a minimum of 15 days before experimentation. Animals were segregated into two groups of 12 each: group a, control; and group b, experimental. All mice received a standard pelleted diet and had free access to food and water. The experimental group wastreated orally for 30 days with Fraxetin (25mg/kg) suspended in 10% (v/v) Tween-20 aqueous solution. |
Dosage form | 25mg/kg; po; 30d |
Applications | Selenium-dependent glutathione reductase activity is significantly increased in the liver of Fraxetin treated mice. |
References: | |
| Cas No. | 574-84-5 | SDF | |
| 别名 | 弗拉西汀 | ||
| 化学名 | 7,8-dihydroxy-6-methoxychromen-2-one | ||
| Canonical SMILES | COC1=C(C(=C2C(=C1)C=CC(=O)O2)O)O | ||
| 分子式 | C10H8O5 | 分子量 | 208.17 |
| 溶解度 | ≥ 20.8mg/mL in DMSO | 储存条件 | Store at 2-8°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.8038 mL | 24.0188 mL | 48.0377 mL |
| 5 mM | 0.9608 mL | 4.8038 mL | 9.6075 mL |
| 10 mM | 0.4804 mL | 2.4019 mL | 4.8038 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet