Home>>Amino Acids & Building Blocks>> N-Protecting Reagents>>Fmoc-Cl

Fmoc-Cl Sale

(Synonyms: 氯甲酸-9-芴基甲酯) 目录号 : GA10139

固相多肽合成

Fmoc-Cl Chemical Structure

Cas No.:28920-43-6

规格 价格 库存 购买数量
25g
¥389.00
现货
100g
¥945.00
现货

电话:400-920-5774 Email: sales@glpbio.cn

Customer Reviews

Based on customer reviews.

Sample solution is provided at 25 µL, 10mM.

产品文档

Quality Control & SDS

View current batch:

实验参考方法

Cell experiment [1]:

Cell lines

Ca9-22 cells

Preparation method

This compound is soluble in water or 1% acetic acid. General tips for obtaining a higher concentration: Please warm the tube at 37 °C for 10 minutes and/or shake it in the ultrasonic bath for a while. Stock solution can be stored below -20 °C for several months.

Reaction Conditions

0.4 μM

Applications

Fmoc-based dipeptide significantly inhibited Ca9-22 cells, with an IC50 value of 0.4 μM. Moreover, this compound had synergistic effect to enhance the antitumor activity of Doxorubicin.

Animal experiment [2]:

Animal models

Rats with carcinogen-induced mammary cancer and mice bearing ER+ human breast cancer xenografts

Dosage form

0.5 μg/mouse; i.p.

Applications

Peptide derived from Fmoc solid-phase synthesis prevented carcinogen-induced mammary cancer in rats and suppressed the growth of ER+ human breast cancer xenografts in mice.

Other notes

Please test the solubility of all compounds indoor, and the actual solubility may slightly differ with the theoretical value. This is caused by an experimental system error and it is normal.

References:

[1]. Yena CT, Wua CC, Leed JC, Chena SL, Morris-Natschkec SL, Hsiehb PW, Wua YC. Cytotoxic N-(fluorenyl-9-methoxycarbonyl) (Fmoc)-dipeptides: Structure–activity relationships and synergistic studies. Eur J Med Chem. 2010 Jun; 45(6): 2494-502.

[2]. Joseph LC, Bennett JA, Kirschner KN, Shields GC, Hughes J, Lostritto N, Jacobsona H, Andersen TT. Antiestrogenic and anticancer activities of peptides derived from the active site of alpha-fetoprotein. J Pept Sci. 2009 Feb; 15: 319-25.

产品描述

IC50: A series of Fmoc-based dipeptides were reported to show cytotoxicity in human cancer cell lines with the IC50 ranges from 0.4 to 1.0 M.

Fmoc-Cl, a chloroformate ester, is commonly applied to introduce Fmoc group during the formation of Fmoc carbamate. Fmoc-based dipeptides are widely explored as a potential anticancer drug. Fmoc protection also serves as a crucial method in solid phase peptide synthesis because it could be removed by piperidine without disturbing the linker between the peptide and the resin. In addition, due to the fluorescent property of Fmoc group, it could react with certain UV-undetectable compounds to form Fmoc derivatives which are feasible for HPLC analysis. [1]

In vitro: Among thirty studied Fmoc-based dipeptides, there were nine compounds intensively against tumor cell growth in human cancer cell lines including HepG2, Hep3B, MCF-7, MDA-MB-231, A549 and Ca9-22. The most active Fmoc-based dipeptide exhibited the highest sensitivity in Ca9-22 cell lines with an IC50 of 0.4 μM, which was 3-fold more potent than doxorubicin. Moreover, this compound had synergistic effect to enhance the antitumor activity of doxorubicin. [1]

In vivo: Peptide derived from Fmoc solid-phase synthesis was reported to have antiestrogenic and anticancer activities. Specifically, intraperitoneal administration of 0.5 μg such peptide had shown to prevent carcinogen-induced mammary cancer in rats and suppress the growth of ER+ human breast cancer xenografts in mice. [2]

Clinical trial: So far, no clinical trial has been conducted.

References:
[1] Yena CT, Wua CC, Leed JC, Chena SL, Morris-Natschkec SL, Hsiehb PW, Wua YC.  Cytotoxic N-(fluorenyl-9-methoxycarbonyl) (Fmoc)-dipeptides: Structure–activity relationships and synergistic studies. Eur J Med Chem. 2010 Jun; 45(6): 2494-502.
[2] Joseph LC, Bennett JA, Kirschner KN, Shields GC, Hughes J, Lostritto N, Jacobsona H, Andersen TT.  Antiestrogenic and anticancer activities of peptides derived from the active site of alpha-fetoprotein. J Pept Sci. 2009 Feb; 15: 319–25.

Chemical Properties

Cas No. 28920-43-6 SDF
别名 氯甲酸-9-芴基甲酯
化学名 9H-fluoren-9-ylmethyl carbonochloridate
Canonical SMILES C1=CC=C2C(=C1)C(C3=CC=CC=C32)COC(=O)Cl
分子式 C15H11ClO2 分子量 258.7
溶解度 ≥ 25.9mg/mL in DMSO, ≥ 24.32 mg/mL in EtOH with ultrasonic 储存条件 Desiccate at -20°C
General tips 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。
储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。
为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。
Shipping Condition 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。

溶解性数据

制备储备液
1 mg 5 mg 10 mg
1 mM 3.8655 mL 19.3274 mL 38.6548 mL
5 mM 0.7731 mL 3.8655 mL 7.731 mL
10 mM 0.3865 mL 1.9327 mL 3.8655 mL
  • 摩尔浓度计算器

  • 稀释计算器

  • 分子量计算器

质量
=
浓度
x
体积
x
分子量
 
 
 
*在配置溶液时,请务必参考产品标签上、MSDS / COA(可在Glpbio的产品页面获得)批次特异的分子量使用本工具。

计算

动物体内配方计算器 (澄清溶液)

第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量)
给药剂量 mg/kg 动物平均体重 g 每只动物给药体积 ul 动物数量
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方)
% DMSO % % Tween 80 % saline
计算重置