Fluorescein-12-dATP
(Synonyms: Fluorescein-12-2’-deoxyadenosine-5’-triphosphate) 目录号 : GC49455
A fluorescently labeled form of dATP
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >95.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Fluorescein-12-dATP is a fluorescently labeled form of the nucleotide 2’-deoxyadenosine-5’-triphosphate . It displays excitation/emission maxima of 498/517 nm, respectively. Fluorescein-12-dATP has been used to label DNA in fluorescence in situ hybridization (FISH) and nicking enzyme-assisted viewing and sequencing (NicE-viewSeq) applications.1,2
1.EstÈve, P.-O., Vishnu, U.S., Chin, H.G., et al.Visualization and sequencing of accessible chromatin reveals cell cycle and PostHDAC inhibitor treatment dynamicsJ. Mol. Biol.432(19)5304-5321(2020) 2.Kasahara, K., Taguchi, T., Yamasaki, I., et al.Genetic alterations in prostate cancerHandbook of immunohistochemistry and in situ hybridization of human carcinomas, Volume 2: Molecular pathology, colorectal carcinoma, and prostate carcinoma2299-305(2005)
| Cas No. | N/A | SDF | Download SDF |
| 别名 | Fluorescein-12-2’-deoxyadenosine-5’-triphosphate | ||
| Canonical SMILES | O=C(CCCCCNC(C1=CC=C(C(O)=O)C(C2=C(C=C3)C(OC4=C2C=CC(O)=C4)=CC3=O)=C1)=O)NCC#CC5=CN([C@]6([H])O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)C6)C7=C5C(N)=NC=N7.O=C(CCCCCNC(C8=CC=C(C9=C(C=C%10)C(OC%11=C9C=CC(O)=C%11)=CC%10=O)C(C(O)=O)=C8)=O)NCC#CC%12=CN([C@]%13([H])O[C@H](COP(OP(OP(O)(O)=O)(O)=O)(O)=O)[C@@H](O)C%13)C%14=C%12C(N)=NC=N%14 | ||
| 分子式 | C41H41N6O19P3 | 分子量 | 1014.7 |
| 溶解度 | Water: soluble | 储存条件 | -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 0.9855 mL | 4.9276 mL | 9.8551 mL |
| 5 mM | 0.1971 mL | 0.9855 mL | 1.971 mL |
| 10 mM | 0.0986 mL | 0.4928 mL | 0.9855 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
NicE-viewSeq: An Integrative Visualization and Genomics Method to Detect Accessible Chromatin in Fixed Cells
Methods Mol Biol 2023;2611:293-302.PMID:36807075DOI:10.1007/978-1-0716-2899-7_16.
A novel genome-wide accessible chromatin visualization, quantitation, and sequencing method is described, which allows in situ fluorescence visualization and sequencing of the accessible chromatin in the mammalian cell. The cells are fixed by formaldehyde crosslinking, and processed using a modified nick translation method, where a nicking enzyme nicks one strand of DNA, and DNA polymerase incorporates biotin-conjugated dCTP, 5-methyl-dCTP, Fluorescein-12-dATP or Texas Red-5-dATP, dGTP, and dTTP. This allows accessible chromatin DNA to be labeled for visualization and on bead NGS library preparation. This technology allows cellular level chromatin accessibility quantification and genomic analysis of the epigenetic information in the chromatin, particularly accessible promoter, enhancers, nucleosome positioning, transcription factor occupancy, and other chromosomal protein binding.