Ethylenediaminetetraacetic acid sodium hydrate
(Synonyms: EDTA sodium hydrate) 目录号 : GC67534
Ethylenediaminetetraacetic acid (EDTA) sodium hydrate 是一种金属螯合剂 (与包括钙在内的二价和三价金属阳离子结合), 具有抗高钙血症和抗凝血活性。Ethylenediaminetetraacetic acid sodium hydrate 可减少金属离子催化的蛋白质氧化损伤，并维持蛋白质纯化过程中的还原环境，常用作蛋白质的纯化和储存。Ethylenediaminetetraacetic acid sodium hydrate 还可减少二硫键的形成。
Cas No.:10378-23-1
Sample solution is provided at 25 µL, 10mM.
;)
,-1-7$$$37316672.jpg');)
;)
;)
$$$36806353.jpg');)
;33(7)%EF%BC%9A546-561$$$37156877.jpg');)
;)
;)
;)
%201124-1138.$$$35908550.jpg');)
%20766-780.$$$37100057.jpg');)
%20418-432.$$$36893736.jpg');)
%EF%BC%9A-240-255.$$$35108512.jpg');)
533-545$$$36543525.jpg');)
%201-13.$$$36600053.jpg');)
;)
Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Ethylenediaminetetraacetic acid (EDTA) sodium hydrate is a metal chelators (binds to metal divalent and trivalent cations including calcium), which shows activities of anticoagulant and anti-hypercalcemic. Ethylenediaminetetraacetic acid sodium hydrate decreases the metal ion-catalyzed oxidative damage to proteins, and allows maintenance of reducing environment during protein purification. Ethylenediaminetetraacetic acid sodium hydrate can also decrease the formation of disulfide bonds[1][2][3].
[1]. Chumanov RS, et al. Artifact-inducing enrichment of ethylenediaminetetraacetic acid and ethyleneglycoltetraacetic acid on anion exchange resins. Anal Biochem. 2011 May 1;412(1):34-9.
[2]. Banfi G, et al. The role of ethylenediamine tetraacetic acid (EDTA) as in vitro anticoagulant for diagnostic purposes. Clin Chem Lab Med. 2007;45(5):565-76.
[3]. Ibad A, et al. Chelation therapy in the treatment of cardiovascular diseases. J Clin Lipidol. 2016 Jan-Feb;10(1):58-62.
| Cas No. | 10378-23-1 | SDF | Download SDF |
| 别名 | EDTA sodium hydrate | ||
| 分子式 | C10H16N2Na4O10 | 分子量 | 416.2 |
| 溶解度 | 储存条件 | Store at -20°C | |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
||
| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 2.4027 mL | 12.0135 mL | 24.0269 mL |
| 5 mM | 0.4805 mL | 2.4027 mL | 4.8054 mL |
| 10 mM | 0.2403 mL | 1.2013 mL | 2.4027 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Final Endodontic Irrigation with 70% Ethanol Enhanced Calcium Hydroxide Removal from the Apical Third
J Endod 2021 Jan;47(1):105-111.PMID:33045271DOI:10.1016/j.joen.2020.09.017.
Introduction: The purpose of this study was to evaluate the cleanliness of root canal walls and dentinal tubules after attempting to remove the calcium hydroxide dressing with different irrigant solutions and the use of nonactivated irrigation or passive ultrasonic irrigation (PUI). Methods: After root canal instrumentation, 80 single-rooted teeth were filled with calcium hydroxide mixed with propylene glycol and 0.1% rhodamine B dye and inserted into canals with a Lentulo spiral. The calcium hydroxide dressing was initially removed with 10 mL saline solution and reinstrumentation with the master apical file. Then, the samples were randomly assigned into 8 experimental groups (n = 10) according to the irrigant solution with or without PUI: 2.5% sodium hypochlorite, 17% Ethylenediaminetetraacetic acid + 1.25% sodium lauryl ether sulfate (EDTA-T), 37% phosphoric acid, or 70% ethanol. A final flush with 5 mL saline solution was performed. The percentage of clean root canal walls and the depth of clean dentinal tubules were measured with images of confocal laser scanning microscopy. The groups were compared using the 2-way analysis of variance test with the Bonferroni post hoc test for depth analysis and the Kruskal-Wallis with Dunn post hoc test for the perimeter analysis. Results: Irrigation with 70% ethanol presented a significantly higher percentage of clean root canal walls and a higher depth of clean dentinal tubules when compared with irrigation with 2.5% sodium hypochlorite and 17% EDTA-T for both irrigation methods (P < .05). No differences were observed between nonactivated irrigation or PUI protocols (P > .05). Conclusions: Seventy percent ethanol enhanced calcium hydroxide removal from the apical root third compared with 2.5% sodium hypochlorite or 17% EDTA-T.
Efficacy of sodium hypochlorite, Ethylenediaminetetraacetic acid, citric acid and phosphoric acid in calcium hydroxide removal from the root canal: a microscopic cleanliness evaluation
Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2011 Dec;112(6):820-4.PMID:22099858DOI:10.1016/j.tripleo.2011.08.001.
Rooted molars were subjected to standardized canal instrumentation to a master apical file (MAF). The samples were dressed with Ca(OH)(2), and after 7 days, teeth were reopened and Ca(OH)(2) medication was removed by 1 of 4 different experimental procedures: 2.5% sodium hypochlorite (NaOCl) (n = 10); 17% EDTA-T (n = 10); 10% citric acid (n = 10); or 37% phosphoric acid (n = 10). This was followed by reinstrumentation with MAF plus 15 mL saline solution. The roots were prepared for scanning electron microscopic analysis of the cervical, middle, and apical thirds. Statistical analysis was performed with the Kruskal-Wallis test. EDTA-T and phosphoric acid gave the best results in the apical third, with significant statistical differences compared with other groups. NaOCl gave the worst results. Irrigation with 17% EDTA-T and 37% phosphoric acid is more effective than sodium hypochlorite and citric acid in the removal of calcium hydroxide from the apical third.
Retrievability of Odontopaste and Metapex With 17% Ethylenediaminetetraacetic acid and 10% Maleic Acid From Root Canals: An Invitro Study
Cureus 2022 Jan 23;14(1):e21508.PMID:35223284DOI:10.7759/cureus.21508.
Aim To evaluate the efficacy of 10% maleic acid in comparison with 17% Ethylenediaminetetraacetic acid (EDTA) in the removal of intracanal medicaments from the root canal system. Materials and methods Forty-eight extracted single-rooted mandibular premolars were decoronated to standardize the length of 14 mm. Chemomechanical preparation was done using the crown-down technique with Protaper files (Dentsply‑Maillefer, Ballaigues, Switzerland) till F4, followed by irrigation with 2 ml of 5.25% sodium hypochlorite (NaOCl) after each instrument, and 5 ml of 17% EDTA was used as the final irrigating agent. Metapex (Meta Dental Corp. Ltd., Elmhurst, NY, USA) and Odontopaste (Australian Dental Manufacturing, Kenmore Hills, Qld, Australia) were the two intracanal medicaments that were used in this study. Total samples were divided into two groups based on the intracanal medicament that was placed in the canal. In group 1, Metapex was injected into the root canal until the material extruded through the apex. In group 2, Odontopaste was placed into the canal until the material extruded through the root apex. Cleaning off the excess medicament was done with a moist cotton pellet. After temporary sealing with a cotton pellet and Cavit, all the samples were stored at 37 ºC and 100% relative humidity for a period of seven days. The teeth in each group were further randomly divided into three subgroups on the basis of the irrigant used for retrieval of medicament. In groups 1A and 2A, 1ml of 17% EDTA was used; in groups 1B and 2B, 1ml of 10% maleic acid was used; in groups 1C and 2C, 1ml of 0.9% saline was used. Sonic agitation for 1 minute, followed by a final rinse of 1 ml distilled water, was used in all the groups. After the intracanal medicament was removed from the canal, the roots were longitudinally sectioned using a diamond disk (Bego, Berman, Germany). The residual medicament on each section was evaluated under a stereomicroscope (×30; Medilux, MDL-DS4-BI, Biosystems, Curitiba, PR, Brazil). The data were analyzed using SPSS version 23.0 software (IBM Corp., Armonk, NY). Kruskal-Wallis ANOVA and Mann-Whitney U test (post hoc) were applied for intergroup comparisons. A Wilcoxon signed-rank test was applied for intragroup comparisons. Results Both the chelators, 17% EDTA and 10% maleic acid, removed the Odontopaste significantly better than Metapex. However, 17% EDTA was more effective in the removal of Odontopaste. 10% Maleic acid showed better results in the removal of Metapex than 17% EDTA. Conclusion None of the chelating agents was able to totally retrieve the intracanal medicaments. When compared to Metapex, Odontopaste showed significantly better retrievability from the root canal with both 17% EDTA and 10% Maleic acid, whereas the retrievability of Metapex was significantly better with 10% Maleic acid in comparison to 17% EDTA.
Simultaneous determination of EDTA, sorbic acid, and diclofenac sodium in pharmaceutical preparations using high-performance liquid chromatography
AAPS PharmSciTech 2013 Jun;14(2):764-9.PMID:23585291DOI:10.1208/s12249-013-9962-0.
A simple high-performance liquid chromatographic method for simultaneous determination of Ethylenediaminetetraacetic acid (EDTA), sorbic acid, and diclofenac sodium was developed and validated. Separation was achieved on a C(18) column (10 cm×4.6 mm) using gradient elution. The mobile phase consisted of acetonitrile-ammonium dihydrogen phosphate buffer solution (0.01 M, pH=2.5, containing 0.8% tetra-n-butyl ammonium hydroxide). The detector wavelength was set at 254 nm. Under these conditions, separation of three compounds was achieved in less than 10 min. The effect of two metal salts and metal concentration on peak area of EDTA was investigated. The pH effect on retention of EDTA and sorbic acid was studied. The method showed linearity for EDTA, sorbic acid, and diclofenac in the ranges of 2.5-100.0, 5.0-200.0, and 20.0-120.0 μg/mL, respectively. The within- and between-day relative standard deviations ranged from 0.52 to 1.94%, 0.50 to 1.34%, and 0.78 to 1.67% for EDTA, sorbic acid, and diclofenac, respectively. The recovery of EDTA, sorbic acid, and diclofenac from pharmaceutical preparation ranged from 96.0-102.0%, 99.7-101.5%, to 97.0-102.5%, respectively. To the best of our knowledge, this is the first report about simultaneous determination of EDTA, sorbic acid, and diclofenac.
Agonistic and Antagonistic Interactions between Chlorhexidine and Other Endodontic Agents: A Critical Review
Iran Endod J 2015 Winter;10(1):1-5.PMID:25598802doi
Root canal irrigants play a significant role in elimination of the microorganisms, tissue remnants, and removal of the debris and smear layer. No single solution is able to fulfill all these actions completely; therefore, a combination of irrigants may be required. The aim of this investigation was to review the agonistic and antagonistic interactions between chlorhexidine (CHX) and other irrigants and medicaments. An English-limited Medline search was performed for articles published from 2002 to 2014. The searched keywords included: chlorhexidine AND sodium hypochlorite/Ethylenediaminetetraacetic acid/calcium hydroxide/mineral trioxide aggregate. Subsequently, a hand search was carried out on the references of result articles to find more matching papers. Findings showed that the combination of CHX and sodium hypochlorite (NaOCl) causes color changes and the formation of a neutral and insoluble precipitate; CHX forms a salt with Ethylenediaminetetraacetic acid (EDTA). In addition, it has been demonstrated that the alkalinity of calcium hydroxide (CH) remained unchanged after mixing with CHX. Furthermore, mixing CHX with CH may enhance its antimicrobial activity; also mixing mineral trioxide aggregate (MTA) powder with CHX increases its antimicrobial activity but this may negatively affect its mechanical properties.