Diethylmaleate
(Synonyms: 马来酸二乙酯) 目录号 : GC11776
马来酸二乙酯是马来酸的两个羧基与乙醇缩合而成的马来酸酯。
Cas No.:141-05-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Diethylmaleate (DEM) is a maleic acid diethyl ester.
DEM is used in many organic syntheses as an additive and intermediate for pigments, plastics, pharmaceuticals, as well as agricultural products. DEM is also an intermediate for the production of adhesives, paints, and copolymers.
In vitro: Previous study found that the GSH depletion by DEM could inhibit the growth of transformed cells. The non-cytotoxic dose of DEM led to GSH depletion, cell cycle arrest, ROS generation, decrease in anchorage independent growth, apoptosis, gene expression changes as well as the activation of all three MAPK family members. In addition, the increased intracellular GSH levels by GSHe opposited the effect of DEM [1].
In vivo: Animal study was conducted to evaluate the effect of DEM induced oxidative stress on male fertility in mice. Results showed that the i.p. injection of DEM for two weeks led to decrease in reduced glutathione and increase in the oxidized glutathione levels in the testis. Moreover, the motility and concentration of sperm were found to be reduced significantly with a obvioius reduction in the litter size. It was also found that expression of redox sensitive transcription factor, cjun and cfos genes, gamma-glutamyl cysteine synthetase and manganese superoxide dismutase expression were affected after DEM treatment, as demonstrated by RT-PCR data. Additionally, the enzyme activity of SOD also increased by the treatment of DEM [2].
Clinical trial: Up to now, there is no clinical data reported for diethyl maleate.
References:
[1] Priya S,Nigam A,Bajpai P,Kumar S. Diethyl maleate inhibits MCA+TPA transformed cell growth via modulation of GSH, MAPK, and cancer pathways. Chem Biol Interact.2014 Aug 5;219:37-47.
[2] Kaur P,Kalia S,Bansal MP. Effect of diethyl maleate induced oxidative stress on male reproductive activity in mice: redox active enzymes and transcription factors expression. Mol Cell Biochem.2006 Oct;291(1-2):55-61.
| Cas No. | 141-05-9 | SDF | |
| 别名 | 马来酸二乙酯 | ||
| 化学名 | diethyl maleate | ||
| Canonical SMILES | CCOC(/C=C\C(OCC)=O)=O | ||
| 分子式 | C8H12O4 | 分子量 | 172.18 |
| 溶解度 | Soluble in DMSO | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
 |
1 mg | 5 mg | 10 mg |
| 1 mM | 5.8079 mL | 29.0394 mL | 58.0788 mL |
| 5 mM | 1.1616 mL | 5.8079 mL | 11.6158 mL |
| 10 mM | 0.5808 mL | 2.9039 mL | 5.8079 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。