Deoxyadenosine 5-triphosphate
(Synonyms: 2'-脱氧腺苷5'-三磷酸酯,dATP) 目录号 : GC13531Deoxyadenosine 5-triphosphate是一种由一个腺嘌呤碱基、一个脱氧核糖和三个磷酸基团组成的脱氧核苷酸三磷酸。
Cas No.:1927-31-7
Sample solution is provided at 25 µL, 10mM.
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Deoxyadenosine 5-triphosphate is a deoxynucleotide triphosphate composed of an adenine base, a deoxyribose and three phosphate groups [1]. Deoxyadenosine 5‑triphosphate is one of the important “raw materials” for DNA polymerase to synthesize DNA chains in DNA replication or in vitro amplification [2]. Deoxyadenosine 5‑triphosphate regulates ribonucleotide reductase by binding to the active site, thereby participating in regulating the balance of deoxynucleoside triphosphate in cells [3].
In Escherichia coli, Deoxyadenosine 5‑triphosphate (400µM; 16h) stimulates bacterial biofilm formation [4].
References:
[1]. Reddy B S, Viswamitra M A. The molecular and crystal structure of the sodium salt of deoxyadenosine-5'-phosphate hexahydrate. Structural Science, 1975, 31(1): 19-26.
[2]. Houghton J A, Tillman D M, Harwood F G. Ratio of 2'-deoxyadenosine-5'-triphosphate/thymidine-5'-triphosphate influences the commitment of human colon carcinoma cells to thymineless death. Clinical cancer research: an official journal of the American Association for Cancer Research, 1995, 1(7): 723-730.
[3]. Alexandrova L A, Skoblov A Y, Jasko M V, et al. 2′-Deoxynucleoside 5′-triphosphates modified at α-, β-and γ-phosphates as substrates for DNA polymerases. Nucleic acids research, 1998, 26(3): 778-786.
[4]. Xi C, Wu J. dATP/ATP, a multifunctional nucleotide, stimulates bacterial cell lysis, extracellular DNA release and biofilm development. PloS one, 2010, 5(10): e13355.
Deoxyadenosine 5-triphosphate是一种由一个腺嘌呤碱基、一个脱氧核糖和三个磷酸基团组成的脱氧核苷酸三磷酸 [1]。Deoxyadenosine 5‑triphosphate是DNA聚合酶在DNA复制或体外扩增中合成DNA链的重要“原料”之一 [2]。Deoxyadenosine 5‑triphosphate通过结合活性位点来调节核苷酸还原酶，从而参与调节细胞内脱氧核苷三磷酸的平衡 [3]。
在大肠杆菌中，Deoxyadenosine 5‑triphosphate（400µM；16h）可刺激细菌生物膜的形成 [4]。
| Bacteria experiment [1]: | |
Bacteria lines | Escherichia coli |
Preparation Method | 1% of overnight Escherichia coli culture was inoculated into the wells of 96-well microplate containing 100µL of 10% LB media supplemented with Deoxyadenosine 5‑triphosphate. After 16h of incubation at 30℃, 15µL of a 1% solution of crystal violet (CV) was added to each well to stain the cells for 15min. Then, the wells were rinsed thoroughly with PBS buffer three times. Biofilm formation was quantified by the addition of 100µL of 95% ethanol to each CV-stained microplate well, and the absorbance was determined with a plate reader at 600nm. |
Reaction Conditions | 400µM; 16h |
Applications | Deoxyadenosine 5‑triphosphate stimulates bacterial biofilm formation. |
References: | |
| Cas No. | 1927-31-7 | SDF | |
| 别名 | 2'-脱氧腺苷5'-三磷酸酯,dATP | ||
| 化学名 | ((2R,3S,5R)-5-(6-amino-9H-purin-9-yl)-3-hydroxytetrahydrofuran-2-yl)methyl tetrahydrogen triphosphate | ||
| Canonical SMILES | NC1=C(N=CN2[C@@]3([H])C[C@@](O)([H])[C@@](O3)([H])COP(O)(OP(O)(OP(O)(O)=O)=O)=O)C2=NC=N1 | ||
| 分子式 | C10H16N5O12P3 | 分子量 | 491.18 |
| 溶解度 | ≥ 50mg/mL in Water | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 2.0359 mL | 10.1796 mL | 20.3591 mL |
| 5 mM | 0.4072 mL | 2.0359 mL | 4.0718 mL |
| 10 mM | 0.2036 mL | 1.018 mL | 2.0359 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet