Delta-like protein 1/DLL1 Protein, Human (HEK293, His)
目录号 : GC27277DLL1 蛋白是 NOTCH1、NOTCH2 和 NOTCH3 受体的跨膜配体，参与顺式和反式相互作用。反式相互作用触发机械力，涉及网格蛋白介导的内吞作用、配体泛素化、EPN1 相互作用和肌动蛋白聚合。Delta-like 蛋白 1/DLL1 蛋白, Human (HEK293, His) 是重组的 Delta 样蛋白 DLL1 蛋白，由 HEK293 表达，带有 C-6*His 标签。
Sample solution is provided at 25 µL, 10mM.
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Delta 样蛋白 1 (DLL1) 作为 NOTCH1、NOTCH2 和 NOTCH3 受体的跨膜配体蛋白，与 Notch 受体的胞外结构域 (ECD) 进行顺式和反式相互作用。相互作用后，配体细胞通过网格蛋白介导的内吞作用施加机械力，包括配体泛素化、EPN1 相互作用和肌动蛋白聚合。这些事件促进Notch受体胞外域(NECD)转内吞作用，触发Notch信号传导，包括Notch受体(NICD)胞内域的裂解、过度磷酸化和核积累。DLL1 对于胚胎发育、维持各种组织和免疫系统中的成体干细胞至关重要。它参与细胞间通讯，调节细胞谱系、模式和形态发生，影响分化和增殖。在大脑发育中，DLL1 在神经元分化和新皮质发育中发挥作用，而在小脑发育中，它调节伯格曼神经胶质单层的形成。它还通过 Notch 信号通路促进免疫系统发育、肌肉发育、胰腺细胞发育、动脉特性维持、血管生成、杯状细胞分化、内耳发育和肾单位发育。DLL1 作为同二聚体发挥作用，并与各种蛋白质相互作用，协调多种细胞过程。
DLL1 protein is a transmembrane ligand for NOTCH1, NOTCH2 and NOTCH3 receptors and participates in cis- and trans-interactions. Trans interactions trigger mechanical forces involving clathrin-mediated endocytosis, ligand ubiquitination, EPN1 interactions, and actin polymerization. Delta-like protein 1/DLL1 Protein, Human (HEK293, His) is the recombinant human-derived Delta-like protein 1/DLL1 protein, expressed by HEK293 , with C-6*His labeled tag.
| Purity | Greater than 95% as determined by reducing SDS-PAGE | Source | HEK293 |
| Phycical Appearance | Lyophilized powder. | Shipping Condition | Room temperature in continental US; may vary elsewhere. |
| Synonyms | rHuDelta-like protein 1/DLL1, His; Delta-like protein 1; Drosophila Delta homolog 1; Delta1; H-Delta-1; DLL1 | ||
| Amino Acid Sequence | QVWSSGVFELKLQEFVNKKGLLGNRNCCRGGAGPPPCACRTFFRVCLKHYQASVSPEPPCTYGSAVTPVLGVDSFSLPDGGGADSAFSNPIRFPFGFTWPGTFSLIIEALHTDSPDDLATENPERLISRLATQRHLTVGEEWSQDLHSSGRTDLKYSYRFVCDEHYYGEGCSVFCRPRDDAFGHFTCGERGEKVCNPGWKGPYCTEPICLPGCDEQHGFCDKPGECKCRVGWQGRYCDECIRYPGCLHGTCQQPWQCNCQEGWGGLFCNQDLNYCTHHKPCKNGATCTNTGQGSYTCSCRPGYTGATCELGIDECDPSPCKNGGSCTDLENSYSCTCPPGFYGKICELSAMTCADGPCFNGGRCSDSPDGGYSCRCPVGYSGFNCEKKIDYCSSSPCSNGAKCVDLGDAYLCRCQAGFSGRHCDDNVDDCASSPCANGGTCRDGVNDFSCTCPPGYTGRNCSAPVSRCEHAPCHNGATCHERGHRYVCECARGYGGPNCQFLLPELPPGPAVVDLTEKLEGQG | ||
| Apparent Molecular Weight | Approximately 65.0 kDa | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose). | ||
| Formulation | Lyophilized from a 0.22 μm filtered solution of 20 mM Hepes, 150 mM NaCl, 1 mM EDTA, pH 7.4. | ||
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.