Defensin HNP-1 human
目录号 : GC64908
An antimicrobial peptide
Cas No.:99287-08-8
Sample solution is provided at 25 µL, 10mM.
Defensin HNP-1 is a peptide secreted by polymorphonuclear leukocytes (PMNs) that has antimicrobial properties.1 It induces lysis of mammalian cells when used at concentrations of 25 and 100 μg/ml, respectively.2 It also completely inhibits secretion of the exotoxin superantigen TSS toxin-1 (TSST-1) from S. aureus when used at a concentration of 50 ng/ml and inhibits anthrax lethal toxin (IC50 = 148 nM).3,4 HNP-1 binds to recombinant HIV-1 envelope glycoprotein (gp120) and human CD4 (Kds = 23.2 and 12.9 nM, respectively).5 It induces lysis and leakage from liposomes composed of dipalmitoyl phosphatidylcholine and dioleyl phosphatidylserine (DOPS).6 In vivo, HNP-1 (5 μg) increases interferon α (IFN-α) production by human plasmacytoid dendritic cells (pDCs) injected into nude mice.7
1.Lehrer, R.I.Primate defensinsNat. Rev. Microbiol.2(9)727-738(2004) 2.Lichtenstein, A., Ganz, T., Selsted, M.E., et al.In vitro tumor cell cytolysis mediated by peptide defensins of human and rabbit granulocytesBlood68(6)1407-1410(1986) 3.Merriman, J.A., Nemeth, K.A., and Schlievert, P.M.Novel antimicrobial peptides that inhibit gram positive bacterial exotoxin synthesisPLoS One9(4)(2014) 4.Wei, G., Pazgier, M., De Leeuw, E., et al.Trp-26 imparts functional versatility to human α-defensin HNP1J. Biol. Chem.285(21)16275-16285(2010) 5.Wang, W., Owen, S.M., Rudolph, D.L., et al.Activity of ɑ- and θ-defensins against primary isolates of HIV-1J. Immunol.173(1)515-520(2004) 6.Fujii, G., Selsted, M.E., and Eisenberg, D.Defensins promote fusion and lysis of negatively charged membranesProtein Sci.2(8)1301-1312(1993) 7.Wang, F., Qiao, L., Lv, X., et al.Alarmin human α defensin HNP1 activates plasmacytoid dendritic cells by triggering NF-κB and IRF1 signaling pathwaysCytokine8353-60(2016)
Cas No. | 99287-08-8 | SDF | Download SDF |
分子式 | C150H228N44O38S6 | 分子量 | 3442.03 |
溶解度 | 储存条件 | Store at -20°C | |
General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液;一旦配成溶液,请分装保存,避免反复冻融造成的产品失效。 储备液的保存方式和期限:-80°C 储存时,请在 6 个月内使用,-20°C 储存时,请在 1 个月内使用。 为了提高溶解度,请将管子加热至37℃,然后在超声波浴中震荡一段时间。 |
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Shipping Condition | 评估样品解决方案:配备蓝冰进行发货。所有其他可用尺寸:配备RT,或根据请求配备蓝冰。 |
制备储备液 | |||
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1 mg | 5 mg | 10 mg |
1 mM | 0.2905 mL | 1.4526 mL | 2.9053 mL |
5 mM | 0.0581 mL | 0.2905 mL | 0.5811 mL |
10 mM | 0.0291 mL | 0.1453 mL | 0.2905 mL |
第一步:请输入基本实验信息(考虑到实验过程中的损耗,建议多配一只动物的药量) | ||||||||||
给药剂量 | mg/kg | 动物平均体重 | g | 每只动物给药体积 | ul | 动物数量 | 只 | |||
第二步:请输入动物体内配方组成(配方适用于不溶于水的药物;不同批次药物配方比例不同,请联系GLPBIO为您提供正确的澄清溶液配方) | ||||||||||
% DMSO % % Tween 80 % saline | ||||||||||
计算重置 |
计算结果:
工作液浓度: mg/ml;
DMSO母液配制方法: mg 药物溶于 μL DMSO溶液(母液浓度 mg/mL,
体内配方配制方法:取 μL DMSO母液,加入 μL PEG300,混匀澄清后加入μL Tween 80,混匀澄清后加入 μL saline,混匀澄清。
1. 首先保证母液是澄清的;
2.
一定要按照顺序依次将溶剂加入,进行下一步操作之前必须保证上一步操作得到的是澄清的溶液,可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
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