DDX58 Protein, Human (His)
目录号 : GC27234DDX58 蛋白是一种先天免疫受体，可检测细胞质病毒核酸并启动信号级联以产生 I 型干扰素。它与病毒 RNA 形成核糖核蛋白复合物并促进同源寡聚化。DDX58 蛋白, Human (His) 是重组的 DDX58 蛋白，由 E. coli 表达，带有 N-6*His 标签。
Sample solution is provided at 25 µL, 10mM.
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| Purity | Greater than 90% as determined by reducing SDS-PAGE. | Source | E. coli |
| Phycical Appearance | Lyophilized powder | Shipping Condition | Room temperature in continental US; may vary elsewhere. |
| Synonyms | Antiviral innate immune response receptor RIG-I; DEAD box protein 58; RLR-1; RIG-I | ||
| Amino Acid Sequence | MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKL | ||
| Apparent Molecular Weight | Approximately 60 kDa | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.22 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4. Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips. | ||
DDX58 蛋白作为先天免疫受体，对于检测细胞质病毒核酸至关重要，启动下游信号级联，导致 I 型干扰素和促炎细胞因子的产生。它与病毒 RNA 形成核糖核蛋白复合物，促进同源寡聚化成丝状。这种寡聚化促进了 RNF135（一种 E3 泛素蛋白连接酶）的募集，从而放大了 RIG-I 介导的抗病毒信号传导。激活后，DDX58 与 MAVS/IPS1 结合，激活 TBK1 和 IKBKE 激酶，导致 IRF3 和 IRF7 磷酸化。这种激活促进抗病毒免疫基因的转录，包括干扰素 IFN-α 和 IFN-β。DDX58 可识别各种病毒 RNA 配体，例如 5'-三磷酸化 ssRNA 和 dsRNA，其中 5'-三磷酸部分和平端碱基配对对其活性至关重要。该蛋白可检测多种 RNA 病毒，并在针对含 dsDNA 病毒（例如 Epstein-Barr 病毒）的抗病毒信号传导中发挥作用。此外，它还参与粒细胞产生、分化、细菌吞噬作用和细胞迁移的调节。
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.