DDX5 Protein, Human (His, Myc)
目录号 : GC27030DDX5 蛋白, Human (His, Myc) 是重组的 DDX5 蛋白，由 E. coli 表达，带有 N-10*His, C-Myc 标签。
Sample solution is provided at 25 µL, 10mM.
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DDX5 蛋白, Human (His, Myc) 是重组的 DDX5 蛋白，由 E. coli 表达，带有 N-10*His, C-Myc 标签。
DDX5 是一种参与 pre-mRNA 剪接替代调控的 RNA 解旋酶，其活性依赖于 RBM4 并促进 tau 外显子 10 的包含。它能结合 tau pre-mRNA 外显子 10 下游的茎环结构区域，且单链 RNA 可显著刺激其 ATP 水解速率。该蛋白还参与转录调控（不依赖解旋酶活性），作为雄激素受体 AR（非 ESR1）的共激活因子，并与 DDX17 和 SRA1 RNA 协同激活 MYOD1 转录活性以促进骨骼肌分化。同时，它是 p53/TP53 的共激活因子，参与 DNA 损伤响应和凋亡，也是 RUNX2 的共激活因子以调控成骨细胞分化。此外，DDX5 能通过与 HDAC1 等组蛋白去乙酰化酶结合实现启动子特异性转录抑制，并作为大型 PER 复合物组分通过抑制 PER1 和 NR1D1 等节律基因的 3' 转录终止来调控昼夜节律。
| Purity | Greater than 95% as determined by reducing SDS-PAGE. | Source | E. coli |
| Phycical Appearance | Lyophilized powder | Shipping Condition | |
| Synonyms | DDX5 蛋白; ATP dependent RNA helicase DDX5; DDX 5; Ddx5 | ||
| Amino Acid Sequence | MSGYSSDRDRGRDRGFGAPRFGGSRAGPLSGKKFGNPGEKLVKKKWNLDELPKFEKNFYQEHPDLARRTAQEVETYRRSKEITVRGHNCPKPVLNFYEANFPANVMDVIARQNFTEPTAIQAQGWPVALSGLDMVGVAQTGSGKTLSYLLPAIVHINHQPFLERGDGPICLVLAPTRELAQQVQQVAAEYCRACRLKSTCIYGGAPKGPQIRDLERGVEICIATPGRLIDFLECGKTNLRRTTYLVLDEADRMLDMGFEPQIRKIVDQIRPDRQTLMWSATWPKEVRQLAEDFLKDYIHINIGALELSANHNILQIVDVCHDVEKDEKLIRLMEEIMSEKENKTIVFVETKRRCDELTRKMRRDGWPAMGIHGDKSQQERDWVLNEFKHGKAPILIATDVASRGLDVEDVKFVINYDYPNSSEDYIHRIGRTARSTKTGTAYTFFTPNNIKQVSDLISVLREANQAINPKLLQLVEDRGSGRSRGRGGMKDDRRDRYSAGKRGGFNTFRDRENYDRGYSSLLKRDFGAKTQNGVYSAANYTNGSFGSNFVSAGIQTSFRTGNPTGTYQNGYDSTQQYGSNVPNMHNGMNQQAYAYPATAAAPMIGYPMPTGYSQ | ||
| Apparent Molecular Weight | Approximately 74.1-75 kDa. | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Human DDX5 at 2.5 μg/mL (100 μL/well) on the plate can bind Anti-DDX5 Antibody. The ED50 for this effect is 36.69 ng/mL. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation | Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, pH 7.4, 8% trehalose. | ||
DDX5 Protein, Human (His, Myc) is the recombinant human-derived DDX5, expressed by E. coli , with N-10*His, C-Myc labeled tag. DDX5 is an RNA helicase involved in the alternative regulation of pre-mRNA splicing, where its helicase activity promotes tau exon 10 inclusion in an RBM4-dependent manner. It binds to the stem-loop region downstream of exon 10 in tau pre-mRNA, with ATP hydrolysis rates highly stimulated by single-stranded RNA. Beyond splicing, DDX5 participates in transcription regulation (independent of helicase activity), acting as a coactivator for androgen receptor AR (but not ESR1) and synergizing with DDX17 and SRA1 RNA to activate MYOD1 transcriptional activity during skeletal muscle differentiation. It also serves as a coactivator for p53/TP53 in DNA damage response and apoptosis, and for RUNX2 in osteoblast differentiation. Additionally, DDX5 exhibits promoter-specific transcriptional repression likely through HDAC1 interaction, and as part of a PER complex, inhibits 3' transcriptional termination of circadian genes like PER1 and NR1D1 to modulate circadian rhythms.
Measured by its binding ability in a functional ELISA. Immobilized Human DDX5 at 2.5 μg/mL (100 μL/well) on the plate can bind Anti-DDX5 Antibody. The ED50 for this effect is 36.69 ng/mL.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.