ddATP trisodium
(Synonyms: 2',3'-Dideoxyadenosine 5'-triphosphate trisodium) 目录号 : GC68952ddATP trisodium 是一种用于分子生物学和生物化学的核苷酸类似物，主要用于 DNA 测序和聚合酶链反应（PCR）
Cas No.:72029-21-1
Sample solution is provided at 25 µL, 10mM.
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ddATP trisodium is a nucleotide analog used in molecular biology and biochemistry, primarily for DNA sequencing and polymerase chain reactions (PCR)[1]. ddATP trisodium is a modified nucleotide analog in which the 3'-hydroxyl group of the 2'-deoxyribose sugar is replaced by a hydrogen atom[2]. This modification prevents the formation of phosphodiester bonds during DNA synthesis, thereby effectively terminating the elongation of the DNA chain[3]. Some studies also suggest that ddATP trisodium can inhibit viral replication[4].
In vitro, treating oocytes with 100μM ddATP trisodium for 5 hours and inducing nuclear DNA double-strand breaks with 0.5μM bleomycin for 1 hour, ddATP trisodium can effectively inhibit mitochondrial DNA (mtDNA) replication in oocytes and reduce γH2AX foci in oocytes[5]. Additionally, studies have found that ddATP trisodium (at concentrations of 1, 2, 4, and 8μmol/mL) can synergistically inhibit the growth of Corynebacterium pseudotuberculosis with penicillin and decrease the minimum inhibitory concentration (MIC) of penicillin against Corynebacterium pseudotuberculosis[6].
References:
[1] Green MR, Sambrook J. Labeling the 3' Termini of Oligonucleotides Using Terminal Deoxynucleotidyl Transferase. Cold Spring Harb Protoc. 2021 Aug 2;2021(8).
[2] Perry CM, Noble S. Didanosine: an updated review of its use in HIV infection. Drugs. 1999 Dec;58(6):1099-135.
[3] Yousaf SI, Carroll AR, Clarke BE. A new and improved method for 3'-end labelling DNA using [alpha-32P]ddATP. Gene. 1984 Mar;27(3):309-13.
[4] Cronn RC, Remington KM, Preston BD, et al. Inhibition of reverse transcriptase from feline immunodeficiency virus by analogs of 2'-deoxyadenosine-5'-triphosphate. Biochem Pharmacol. 1992 Oct 6;44(7):1375-81.
[5] Ma JY, Feng X, Xie FY, et al. Double-strand breaks induce short-scale DNA replication and damage amplification in the fully grown mouse oocytes. Genetics. 2021 Jun 24;218(2):iyab054.
[6] Bernardino PN, de Paula CL, Pereira AFM, et al. Potential in vitro action of an adenosine analog and synergism with penicillin against Corynebacterium pseudotuberculosis. Braz J Microbiol. 2023 Mar;54(1):559-563.
ddATP trisodium 是一种用于分子生物学和生物化学的核苷酸类似物，主要用于 DNA 测序和聚合酶链反应（PCR）[1]。ddATP trisodium 是一种经过修饰的核苷酸类似物，其 2'-脱氧核糖的 3'-羟基被氢原子取代[2]。这种修饰使得 DNA 合成过程中无法形成磷酸二酯键，从而有效终止 DNA 链的延伸[3]。也有研究认为ddATP trisodium可抑制病毒的复制[4]。
在体外，用100μM ddATP trisodium处理卵母细胞5小时，用0.5μM博来霉素诱导核DNA双链断裂1小时，ddATP trisodium可有效抑制卵母细胞的线粒体DNA(mtDNA)复制，并且ddATP trisodium降低卵母细胞的γH2AX病灶[5]。也有研究发现ddATP trisodium（1、2、4、8μmol/mL）可协同青霉素抑制假结核棒状杆菌的生长能力，并降低假结核棒状杆菌对青霉素的最小抑菌浓度（MIC）[6]。
| Cell experiment [1]: | |
Cell lines | MII oocytes |
Preparation Method | Treating oocytes with 100μM ddATP trisodium for 5 hours and inducing nuclear DNA double-strand breaks with 0.5μM bleomycin for 1 hour. |
Reaction Conditions | 100μM; 5h. |
Applications | ddATP trisodium can effectively inhibit mitochondrial DNA (mtDNA) replication in oocytes and reduce γH2AX foci in oocytes. |
References: | |
| Cas No. | 72029-21-1 | SDF | Download SDF |
| 别名 | 2',3'-Dideoxyadenosine 5'-triphosphate trisodium | ||
| 分子式 | C10H13N5Na3O11P3 | 分子量 | 541.13 |
| 溶解度 | H2O : 125 mg/mL (231.00 mM; Need ultrasonic) | 储存条件 | Solution, -20°C, 2 years |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.848 mL | 9.2399 mL | 18.4798 mL |
| 5 mM | 0.3696 mL | 1.848 mL | 3.696 mL |
| 10 mM | 0.1848 mL | 0.924 mL | 1.848 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >99.00%
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