D-Allose
(Synonyms: D-阿洛糖) 目录号 : GC62921
D-Allose是一种超低热量的醛己糖，在冷冻过程中对细胞存活具有冷冻保护功能。
Cas No.:2595-97-3
Sample solution is provided at 25 µL, 10mM.
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D-Allose is an ultra-low-calorie aldohexose that exhibits cryoprotective function on cell survival during freezing [1]. D-Allose protects mouse hippocampal HT-22 cells from oxygen-glucose deprivation and reperfusion (OGD/R) -induced injury by inhibiting the Gal-3/TLR4 signalling pathway [2]. D-Allose has been widely used to inhibit the proliferation of a variety of cancer cells and prevent cancer cells from taking up glucose[3].
In vitro, D-Allose treatment for 48 hours significantly inhibited the viability of MIA PaCa-2 cells with an IC50 value of 53.25mM[4]. Treatment of cells with 40mM D-Allose for 48 hours inhibited the growth of PC-3 cells, induced apoptosis, and caused cell cycle arrest[5]. Treatment of RT112 cells with 50mM D-Allose for 24h inhibited cell viability, induced intracellular reactive oxygen species (ROS) production, and stimulated TXNIP expression[6].
In vivo, D-Allose treatment via intraperitoneal injection at a dose of 100mg/kg/day for 28 days significantly reduced tumor volume in a subcutaneous U87MG cell xenograft mouse model [7]. Intravenous administration of 0.4mg/g D-Allose 1 hour before ischemia significantly inhibited apoptosis, reduced blood brain barrier (BBB) permeability, and decreased inflammatory cytokine levels in the mice model of middle cerebral artery occlusion and reperfusion (MCAO/Rep) [8].
References:
[1] Chen Z, Chen J, Zhang W, et al. Recent research on the physiological functions, applications, and biotechnological production of D-allose[J]. Applied microbiology and biotechnology, 2018, 102(10): 4269-4278.
[2] Huang Y, Thonusin C, Tokuda M, et al. The beneficial effects of D-allose and D-allulose on the brain under ischemic stroke and obese-insulin resistant conditions: evidence from in vitro to clinical studies[J]. Metabolic Brain Disease, 2025, 40(4): 1-15.
[3] Noguchi C, Kamitori K, Hossain A, et al. D-allose inhibits cancer cell growth by reducing GLUT1 expression[J]. The Tohoku journal of experimental medicine, 2016, 238(2): 131-141.
[4] Malm S W, Hanke N T, Gill A, et al. The anti-tumor efficacy of 2-deoxyglucose and D-allose are enhanced with p38 inhibition in pancreatic and ovarian cell lines[J]. Journal of Experimental & Clinical Cancer Research, 2015, 34(1): 31.
[5] Naha N, Lee H Y, Jo M J, et al. Rare sugar D-allose induces programmed cell death in hormone refractory prostate cancer cells[J]. Apoptosis, 2008, 13(9): 1121-1134.
[6] Tohi Y, Taoka R, Zhang X, et al. Antitumor effects of orally administered rare sugar D-allose in bladder cancer[J]. International Journal of Molecular Sciences, 2022, 23(12): 6771.
[7] Suzuki K, Ogawa D, Kanda T, et al. Antiproliferative effects of D-allose associated with reduced cell division frequency in glioblastoma[J]. Scientific Reports, 2023, 13(1): 19515.
[8] Huang T, Gao D, Hei Y, et al. D-allose protects the blood brain barrier through PPARγ-mediated anti-inflammatory pathway in the mice model of ischemia reperfusion injury[J]. Brain research, 2016, 1642: 478-486.
D-Allose是一种超低热量的醛己糖，在冷冻过程中对细胞存活具有冷冻保护功能[1]。D-Allose通过抑制Gal-3/TLR4信号通路，保护小鼠海马HT-22细胞免受氧糖剥夺/再灌注（OGD/R）诱导的损伤[2]。D-Allose已被广泛用于抑制多种癌细胞的增殖并阻止癌细胞摄取葡萄糖[3]。
在体外，D-Allose处理48小时显著抑制了MIA PaCa-2细胞的活力，IC50值为53.25 mM[4]。使用40 mM D-阿洛糖处理细胞48小时，抑制了PC-3细胞的生长，诱导了细胞凋亡，并引起细胞周期阻滞[5]。使用50mM的D-Allose处理RT112细胞24小时，抑制了细胞活力，诱导了细胞内活性氧（ROS）的产生，并刺激了TXNIP的表达[6]。
在体内，每日腹腔注射100mg/kg剂量的D-Allose，持续28天，显著降低了皮下U87MG细胞异种移植小鼠模型中的肿瘤体积[7]。在缺血前1小时静脉注射0.4mg/g剂量的D-Allose，显著抑制了小鼠大脑中动脉闭塞/再灌注（MCAO/Rep）模型中的细胞凋亡，降低了血脑屏障（BBB）通透性，并减少了炎症细胞因子水平[8]。
| Cell experiment [1]: | |
Cell lines | MIA PaCa-2 cells |
Preparation Method | MIA PaCa-2 cells were cultured in Dulbecco's Modified Eagle's medium (DMEM) supplemented with 10% fetal bovine serum and 4.5g/l glucose. Cells were seeded in 96-well plates at a density of 3000 cells/well, with the lateral wells left empty for water addition. Cells were treated with different concentrations of D-Allose (0.01, 0.1, 1,10, and 100mM) for 48 hours, and cell proliferation was analyzed. |
Reaction Conditions | 0.01, 0.1, 1,10, and 100mM; 48h |
Applications | D-Allose treatment decreased the cell viability of MIA PaCa-2 cells in a dose-dependent manner. |
| Animal experiment [2]: | |
Animal models | Male BALB/c (nu/nu) nude mice |
Preparation Method | Male BALB/c (nu/nu) nude mice, 5 weeks old and weighing 20g, were housed in a specific pathogen clearance grade (SPF) animal house at 24±2°C temperature and 55±5% humidity and maintained on a 12h light / 12h dark circadian rhythm. Mice received a standard diet and water AD libitum. U87MG cells (5×106 cells/100µl) were injected subcutaneously into the right abdomen of mice. When the tumor volume reached about 150mm3, the mice were randomly divided into two groups: the experimental group received D-Allose (100mg/kg; i.p.) once a day for 30 consecutive days; mice in the control group were intraperitoneally injected with normal saline. Tumor volume and body weight of mice were analyzed. |
Dosage form | 100mg/kg/day for 30 days; i.p. |
Applications | D-Allose treatment suppressed tumor growth in the U87MG cell xenograft mouse models without affecting body weight. |
References: | |
| Cas No. | 2595-97-3 | SDF | |
| 别名 | D-阿洛糖 | ||
| 分子式 | C6H12O6 | 分子量 | 180.16 |
| 溶解度 | DMSO : 100 mg/mL (555.06 mM; Need ultrasonic); H2O : 100 mg/mL (555.06 mM; Need ultrasonic) | 储存条件 | Store at 2-8°C,protect from light |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 5.5506 mL | 27.7531 mL | 55.5062 mL |
| 5 mM | 1.1101 mL | 5.5506 mL | 11.1012 mL |
| 10 mM | 555.1 μL | 2.7753 mL | 5.5506 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
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- Purity: >97.00%
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