Coumarin 6H
(Synonyms: 香豆素 6H；香豆素6H) 目录号 : GC67744Coumarin 6H 是一种 Coumarin (Coumarin ) 衍生物，是一种激光染料。Coumarin 6H 的荧光发射可以通过氢键相互作用增强。
Cas No.:58336-35-9
Sample solution is provided at 25 µL, 10mM.
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Quality Control & SDS
- View current batch:
- Purity: >98.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet
Coumarin 6H, a Coumarin (Coumarin ) derivative, is a laser dye. The fluorescence emission of Coumarin 6H can be enhanced by hydrogen bonding interactions[1][2].
具有扭曲嵌段和更强供电子久洛利定基团的 Coumarin 6H 在被荧光猝灭基团抑制时表现出更高的量子产率、更好的生物相容性以及更少的背景荧光。因此，探针 LH-1 中的 Coumarin 6H 可能有助于降低背景荧光。在探针LH-1中，Coumarin 6H 荧光团的荧光不仅可以被 N-N 基团的旋转猝灭，还可以通过供体激发光诱导电子转移 (d-PET) 机制被 2,4-二硝基苯基团猝灭[2]。
[1]. P M French, et al. Passively mode-locked cw Coumarin 6 ring dye laser. Opt Lett. 1989 Feb 15;14(4):217-8.
[2]. Zhixiang Han, et al. A novel fluorescent probe with extremely low background fluorescence for sensing hypochlorite in zebrafish. Anal Biochem. 2020 Aug 1;602:113795.
| Cas No. | 58336-35-9 | SDF | Download SDF |
| 别名 | 香豆素 6H；香豆素6H | ||
| 分子式 | C15H15NO2 | 分子量 | 241.29 |
| 溶解度 | DMSO : 12.5 mg/mL (51.80 mM; ultrasonic and warming and heat to 60°C) | 储存条件 | Store at -20°C |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 4.1444 mL | 20.722 mL | 41.4439 mL |
| 5 mM | 0.8289 mL | 4.1444 mL | 8.2888 mL |
| 10 mM | 0.4144 mL | 2.0722 mL | 4.1444 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Coumarin 6H-fused fluorescent probe for highly sensitive detection of coralyne using oligonucleotide-modified silver nanoparticles
Anal Bioanal Chem 2022 Oct;414(24):7299-7313.PMID:35976422DOI:10.1007/s00216-022-04282-2.
In this study, a novel, rapid, and sensitive fluorescence sensing platform was developed for the detection of coralyne (COR) by the conjugation of Coumarin 6H (C6H) fluorescent dye with oligonucleotide-modified silver nanoparticles [(dT)32-AgNPs]. In the presence of COR, a remarkable and rapid decrease in the fluorescence signal of the probe with a quenching efficiency of around 62% was observed. The quenching response of the system towards COR was possibly due to the displacement of thymidine-rich deoxyoligonucleotides by COR on the surface of AgNPs. The complementary experiments with an adenine-rich single strand as well as with two different secondary structures (i.e., duplex and triplex) revealed a favorable sequence specificity of the sensing platform. The influence of key parameters including the incubation time and temperature was evaluated and optimized to achieve the highest performance. The linear range of 10-183 nM with a correlation coefficient of R = 0.9982 and a limit of detection of 5.24 nM were obtained under the optimized conditions. The selectivity of the proposed probe towards COR was revealed by the evaluation of its response to other small molecules that have molecular structures similar to COR. Finally, the successful applicability of the system was shown with the obtained average recoveries in the range of 87.28-104.52% in human urine samples.