Collagenase I
(Synonyms: 胶原蛋白酶,Collagenase; collagen hydrolase) 目录号 : GC19589胶原酶I型（Collagenase I），≥125 CDU/mg 固体（CDU =胶原蛋白消化单位）
Sample solution is provided at 25 µL, 10mM.
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Source: Bacillus histolyticus
Activity definition: One FALGPA hydrolysis unit hydrolyzes 1.0 μmole of furylacryloyl-Leu-Gly-Pro-Ala per min at pH 7.5 at 25 °C in the presence of calcium ions. One neutral protease unit hydrolyzes casein to produce color equivalent to 1.0 μmole tyrosine per 5 hr at pH 7.5 at 37℃. One clostripain unit hydrolyzes 1.0 μmole of BAEE per min at pH 7.6 at 25℃ in the presence of DTT
Optimal pH value: 7～8
Uses: It has the unique ability to digest natural collagen and denatured collagen. Detergents, hexachlorocyclohexane and heavy metal ions can reduce enzyme activity. It acts exclusively on procollagen, breaking it and then being hydrolyzed by other proteases, without hydrolyzing fibrin and globulin
| Cas No. | 9001-12-1 | SDF | |
| 别名 | 胶原蛋白酶,Collagenase; collagen hydrolase | ||
| 分子式 | C12H18ClNO3 | 分子量 | 259.73 |
| 溶解度 | >5mg/mL in water | 储存条件 | Store at -20°C |
| General tips | For obtaining a higher solubility , please warm the tube at 37 ℃ and shake it in the ultrasonic bath for a while. | ||
| Shipping Condition | Evaluation sample solution : ship with blue ice All other available size: ship with RT , or blue ice upon request |
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| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 3.8502 mL | 19.2508 mL | 38.5015 mL |
| 5 mM | 0.77 mL | 3.8502 mL | 7.7003 mL |
| 10 mM | 0.385 mL | 1.9251 mL | 3.8502 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。