Coenzyme A
(Synonyms: 辅酶 A) 目录号 : GC43293
Coenzyme A是一种活细胞中不可或缺的辅因子，由泛酸（维生素B5）、三磷酸腺苷（ATP）和半胱氨酸合成。
Cas No.:85-61-0
Sample solution is provided at 25 µL, 10mM.
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Coenzyme A is an indispensable cofactor in living cells, synthesized from pantothenic acid (vitamin B5), adenosine triphosphate (ATP), and cysteine [1]. As an acyl carrier and carbonyl activating group, Coenzyme A plays a crucial role in the oxidation of pyruvate in the citric acid cycle and the metabolism of carboxylic acids (including short-chain and long-chain fatty acids) [2]. Abnormalities in the biosynthesis and homeostasis of Coenzyme A and its derivatives are associated with various human diseases, including cancer, diabetes, and neurodegeneration [3].
In vitro, supplementing Coenzyme A (25μM; 30 days) in the culture medium can significantly restore the function and mitochondrial respiratory activity of neuronal cells from patients with Pantothenate kinase-associated neurodegenerative disease (PKAN), while inhibiting neuronal death and ROS formation [4]. Coenzyme A (1mM; 30min) can completely inhibit the activity of peroxiredoxin 5 (Prdx5), and this inhibitory state can be relieved by dithiothreitol (DTT) reduction [5].
References:
[1] Michal G, Bergmeyer H U. Coenzyme A[M]//Methods of enzymatic analysis. Academic Press, 1974: 1967-1987.
[2] Leonardi R, Zhang YM, Rock CO, Jackowski S. Coenzyme A: back in action. Prog Lipid Res. 2005;44(2-3):125-153.
[3] Tsuchiya Y, Peak-Chew SY, Newell C, et al. Protein CoAlation: a redox-regulated protein modification by coenzyme A in mammalian cells. Biochem J. 2017;474(14):2489-2508. Published 2017 Jul 11.
[4] Orellana DI, Santambrogio P, Rubio A, et al. Coenzyme A corrects pathological defects in human neurons of PANK2-associated neurodegeneration. EMBO Mol Med. 2016;8(10):1197-1211.
[5] Baković J, Yu B Y K, Silva D, et al. A key metabolic integrator, coenzyme A, modulates the activity of peroxiredoxin 5 via covalent modification[J]. Molecular and cellular biochemistry, 2019, 461(1): 91-102.
Coenzyme A是一种活细胞中不可或缺的辅因子，由泛酸（维生素B5）、三磷酸腺苷（ATP）和半胱氨酸合成 [1]。Coenzyme A作为酰基载体和羰基活化基团，在柠檬酸循环中丙酮酸的氧化以及羧酸（包括短链和长链脂肪酸）的代谢过程中起重要作用 [2]。Coenzyme A及其衍生物的生物合成和稳态异常与多种人类疾病有关，包括癌症、糖尿病和神经退行性变 [3]。
在体外，在培养基中补充Coenzyme A（25μM; 30 days）能够显著恢复来自Pantothenate激酶相关神经退行性疾病（PKAN）患者的神经元细胞的功能和线粒体呼吸活性，同时抑制神经元死亡和ROS形成 [4]。Coenzyme A（1mM; 30min）能够完全抑制过氧化物酶5（Prdx5）的活性，这种抑制状态可通过二硫苏糖醇（DTT）还原来解除 [5]。
| Cell experiment [1]: | |
Cell lines | Human neuronal precursors cells and neurons (NPCs) |
Preparation Method | NPCs from Pantothenate kinase‐associated neurodegeneration (PKAN) patients were seeded on Matrigel‐coated wells and differentiated in medium containing Neurobasal, BDNF (10ng/ml), NT‐3 (10ng/ml), B27, P/S, and doxycycline (2μg/ml). A final concentration of 25μM Coenzyme A was added to treat the cells. Coenzyme A was resuspended in a Neurobasal medium and stored at −20°C. Fresh medium containing Coenzyme A (25μM) was added every 2 days to the cells undergoing treatment. Measure the mitochondrial membrane potential, ROS, the morphology of dendritic branches, and determine the activity of aconitase in neurons. |
Reaction Conditions | 25μM; 30 days |
Applications | Supplementation of Coenzyme A in the culture medium can significantly restore the function of neurons related to PKAN disease and the mitochondrial respiratory activity, preventing neuronal death and ROS formation. |
References: | |
| Cas No. | 85-61-0 | SDF | |
| 别名 | 辅酶 A | ||
| Canonical SMILES | O[C@H]1[C@H](N2C=NC3=C2N=CN=C3N)O[C@H](COP(OP(OCC(C)(C)[C@@H](O)C(NCCC(NCCS)=O)=O)(O)=O)(O)=O)[C@H]1OP(O)(O)=O | ||
| 分子式 | C21H36N7O16P3S | 分子量 | 767.5 |
| 溶解度 | PBS (pH 7.2): 10 mg/ml | 储存条件 | Store at -20°C, protect from light, filled inert atmosphere |
| General tips | 请根据产品在不同溶剂中的溶解度选择合适的溶剂配制储备液；一旦配成溶液，请分装保存，避免反复冻融造成的产品失效。 储备液的保存方式和期限：-80°C 储存时，请在 6 个月内使用，-20°C 储存时，请在 1 个月内使用。 为了提高溶解度，请将管子加热至37℃，然后在超声波浴中震荡一段时间。 |
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| Shipping Condition | 评估样品解决方案：配备蓝冰进行发货。所有其他可用尺寸：配备RT，或根据请求配备蓝冰。 | ||
| 制备储备液 | |||
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1 mg | 5 mg | 10 mg |
| 1 mM | 1.3029 mL | 6.5147 mL | 13.0293 mL |
| 5 mM | 260.6 μL | 1.3029 mL | 2.6059 mL |
| 10 mM | 130.3 μL | 651.5 μL | 1.3029 mL |
| 第一步：请输入基本实验信息（考虑到实验过程中的损耗，建议多配一只动物的药量） | ||||||||||
| 给药剂量 | mg/kg |  |
动物平均体重 | g | |
每只动物给药体积 | ul | |
动物数量 | 只 |
| 第二步：请输入动物体内配方组成（配方适用于不溶于水的药物；不同批次药物配方比例不同，请联系GLPBIO为您提供正确的澄清溶液配方） | ||||||||||
| % DMSO % % Tween 80 % saline | ||||||||||
| 计算重置 | ||||||||||
计算结果:
工作液浓度： mg/ml；
DMSO母液配制方法： mg 药物溶于 μL DMSO溶液（母液浓度 mg/mL，
体内配方配制方法：取 μL DMSO母液，加入 μL PEG300，混匀澄清后加入μL Tween 80，混匀澄清后加入 μL saline，混匀澄清。
1. 首先保证母液是澄清的；
2.
一定要按照顺序依次将溶剂加入，进行下一步操作之前必须保证上一步操作得到的是澄清的溶液，可采用涡旋、超声或水浴加热等物理方法助溶。
3. 以上所有助溶剂都可在 GlpBio 网站选购。
Quality Control & SDS
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- Purity: >85.00%
- COA (Certificate Of Analysis)
- SDS (Safety Data Sheet)
- Datasheet