CleanCap Reagent GG (3’ OMe)
目录号 : GN20153
CleanCap Reagent GG (3’ OMe) is designed for the cotranscriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1
.
Sample solution is provided at 25 µL, 10mM.
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CleanCap Reagent GG (3’ OMe) is designed for the cotranscriptional capping of mRNA to produce an mRNA with naturally occurring Cap 1. Cap 1 mRNAs have superior in vivo activity compared to Cap 0 mRNA produced by legacy capping methods such as mCap or anti-reverse cap analog (ARCA).
CleanCap GG (3’ OMe) includes the 5’ N7-Methyl-3’-O-Methylguanosine commonly found in mRNA capped using ARCA, whereas CleanCap GG has a naturally occurring 5’ N7-Methylguanosine structure.
CleanCap can be used in conjunction with GlpBio’s catalog of modified NTPs.
Using the recommended conditions, transcription with CleanCap GG (3’OMe) results in 70-90% capped material, generating a Cap 1 structure and gives crude yields of ~1.5 mg per mL of transcription. Final capping is dependent upon the DNA template and final mRNA sequence. Secondary structure due to RNA length and base composition can affect final capping efficiency.
| purity | >95.00% | Extinction Coefficient | 31,700 Lmol-1cm-1 at 255 nm |
| 分子式 | C33H45N15O25P4 (free acid) | 分子量 | 1175.7 g/mole (free acid) |
| Salt Form | Na+ | Concentration | 50 mM |
| Buffer | H2O | 储存条件 | -20°C or below |
| Synonyms | Backbone | 5'-5'-Triphosphate | |
| Base Analog | Guanosine | Sugar Type | RNA |
| Nucleotide Category | Cap analogs | ||
| Applications | CRISPR, In vitro Transcription, Recombinases, TALENS, Transposases, Vaccine Development | ||