CD20/MS4A1 Protein, Mouse (P.pastoris, His)
目录号 : GC27081CD20/MS4A1 蛋白调节细胞钙流入，这对于 B 淋巴细胞的发育、分化和激活至关重要。它激活钙池操纵的钙通道，通过 B 细胞受体/BCR 激活允许钙流入。
Sample solution is provided at 25 µL, 10mM.
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CD20/MS4A1 蛋白调节细胞钙流入，这对于 B 淋巴细胞的发育、分化和激活至关重要。它激活钙池操纵的钙通道，通过 B 细胞受体/BCR 激活允许钙流入。CD20/MS4A1 蛋白形成同源四聚体，并与细胞表面 IgM 链（BCR 的抗原结合部分）相互作用。CD20/MS4A1 蛋白, Mouse (P.pastoris, His) 是重组的 CD20/MS4A1 蛋白，由 P. pastoris 表达，带有 N-6*His 标签。
CD20/MS4A1 蛋白是一种 B 淋巴细胞特异性膜蛋白，在调节细胞钙内流方面发挥着至关重要的作用，这对于 B 淋巴细胞的发育、分化和激活是必需的。它作为钙库操纵 (SOC) 通道的一个组成部分发挥作用，在 B 细胞受体/BCR 激活后促进钙流入。CD20/MS4A1 蛋白形成同源四聚体，并与细胞表面 IgM 的重链和轻链相互作用，IgM 是 BCR 的抗原结合成分。
| Purity | Greater than 90% as determined by reducing SDS-PAGE. | Source | P. pastoris |
| Phycical Appearance | Lyophilized powder | Shipping Condition | |
| Synonyms | CD20 蛋白/MS4A1 蛋白; Ms4a1; Cd20; Ly-44; Ms4a2; B-lymphocyte antigen CD20; B-cell differentiation antigen Ly-44; Lymphocyte antigen 44; CD antigen | ||
| Amino Acid Sequence | VIMSSLSLFAAISGIILSIMDILNMTLSHFLKMRRLELIQTSKPYVDIYDCEPSNSSEKNSPSTQYCNSIQSVFLGILSAMLISAFFQKLVTAGIVENEWKRMCTRSKSNVVLLSAGEKNEQTIKMKEEIIELSGVSSQPKNEEEIEIIPVQEEEEEEAEINFPAPPQEQESLPVENEIAP | ||
| Apparent Molecular Weight | Approximately 33 kDa.The reducing (R) protein migrat es as 33 kDa in SDS-PAGE may be due to glycosylation. | ||
| Stability | Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage. | ||
| Biological Activity | Measured by its binding ability in a functional ELISA. Immobilized Mouse CD20, at 2 μg/mL (100 μL/well) can bind Anti-CD20 antibody. The ED50 for this effect is 1.066 μg/mL. | ||
| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. | ||
| Formulation |
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4. Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled c |
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CD20/MS4A1 protein regulates cellular calcium influx, essential for B-lymphocyte development, differentiation, and activation. It activates store-operated calcium channels, allowing calcium influx through B-cell receptor/BCR activation. CD20/MS4A1 protein forms homotetramers and interacts with cell surface IgM chains, the antigen-binding parts of the BCR. CD20/MS4A1 Protein, Mouse (P.pastoris, His) is the recombinant mouse-derived CD20/MS4A1 protein, expressed by P. pastoris , with N-6*His labeled tag.
Measured by its binding ability in a functional ELISA. Immobilized Mouse CD20, at 2 μg/mL (100 μL/well) can bind Anti-CD20 antibody. The ED50 for this effect is 1.066 μg/mL.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.