Cas9 Protein, Streptococcus pyogenes M1 (sf9, NLS, His)
目录号 : GC27016Cas9 蛋白是免疫系统的一部分，可防御遗传因素。它处理 RNA 并帮助切割 DNA 靶标。蛋白质和向导 RNA 是其功能所必需的。
Sample solution is provided at 25 µL, 10mM.
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Cas9 蛋白是免疫系统的一部分，可防御遗传因素。它处理 RNA 并帮助切割 DNA 靶标。蛋白质和向导 RNA 是其功能所必需的。该蛋白质可识别特定序列以区分自身与异物，并提供针对匹配遗传元件的免疫力。Cas9 蛋白, Streptococcus pyogenes M1 (sf9, NLS, His) 是重组的 Cas9 蛋白，由 Sf9 insect cells 表达，带有 C-His 标签。
Cas9 是一种革命性的分子工具，它构成了一种适应性免疫系统，可以防御病毒、转座元件和接合质粒等移动遗传元件。该过程涉及 crRNA 的转录和加工，其中，对于 II 型基因编辑系统，前 crRNA 的正确加工需要反式编码的小 RNA (tracrRNA)、内源性核糖核酸酶 3 (rnc) 和关键的 Cas9蛋白质。tracrRNA 引导核糖核酸酶 3 处理 pre-crRNA，Cas9 稳定 pre-crRNA:tracrRNA 相互作用。在后续步骤中，Cas9/crRNA/tracrRNA 复合物充当核酸内切酶，用互补的间隔序列切割靶 DNA。如果没有两个特定引导 RNA (gRNA) 的指导，Cas9 就会失活，这强调了这两种 gRNA 对于 DNA 结合和核酸酶活性的必要性。这个复杂的系统通过一种称为基因编辑干扰的机制赋予对入侵遗传元件的免疫力。
| Purity | Greater than 90% as determined by reducing SDS-PAGE. | Source | Sf9 insect cells |
| Phycical Appearance | Solution. | Shipping Condition | Shipping with dry ice. |
| Synonyms | Cas9; endonuclease Cas9/Csn1; SpyCas9 | ||
| Amino Acid Sequence | MPAAKRVKLDMDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKNLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESFLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKFRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQIGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLRKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGNSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYFTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDSVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERLKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRHKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYLYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPSEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHVAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLNAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTEITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGITIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNELALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLDATLIHQSITGLYETRIDLSQLGGDPAAKRVKLDPAAKRVKLD | ||
| Apparent Molecular Weight | Approximately 163 kDa | ||
| Stability | Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles. | ||
| Biological Activity |
1. 1 μg = 5 U ,1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃. 2. Cas9 Protein achieves ≥ 90% substrate cleavage measured by an in vitro DNA cleavage assay. |
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| Endotoxin Level | <1 EU/μg, determined by LAL method. | ||
| Reconstitution | N/A. | ||
| Formulation | Supplied as a 0.22 μm filtered solution of 20 mM Tris, 300 mM NaCl, 20-50 % glycerol, pH 8.0, 0.5 mM PMSF, 0.1 mM EDTA, 0.5 mM TCEP. | ||
The Cas9 protein is part of an immune system that defends against genetic elements.It processes RNA and aids in cleaving DNA targets.Protein and guide RNAs are necessary for its function.The protein recognizes specific sequences to distinguish self from nonself and provides immunity against matching genetic elements. Cas9 Protein, Streptococcus pyogenes M1 (sf9, NLS, His) is the recombinant Cas9 protein, expressed by Sf9 insect cells , with C-His labeled tag.
1. 1 μg = 5 U ,1 U is defined as cutting 1 ug 1 KB dsDNA in 30 min at 37℃.
2. Cas9 Protein achieves ≥ 90% substrate cleavage measured by an in vitro DNA cleavage assay.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.